A5004709668- Pancreatic function and diabetes
- Diabetes and associated disorders
- Diabetes Treatment and Management
- Pancreatitis Pathology and Treatment
- Diet, Metabolism, and Disease
- Metabolism, Diabetes, and Cancer
- Diabetes Management and Research
- Cannabis and Cannabinoid Research
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Signaling Pathways in Disease
- MicroRNA in disease regulation
- Transgenic Plants and Applications
- Viral gastroenteritis research and epidemiology
- Autophagy in Disease and Therapy
- Light effects on plants
- Neuropeptides and Animal Physiology
- Food Safety and Hygiene
- Neurogenetic and Muscular Disorders Research
- Heme Oxygenase-1 and Carbon Monoxide
- Natural Antidiabetic Agents Studies
- Insect symbiosis and bacterial influences
- NF-κB Signaling Pathways
- Salmonella and Campylobacter epidemiology
- Neuroscience and Neural Engineering
Gyeongsang National University
2016-2024
Catholic University of Korea
2008-2023
Chung-Ang University
2003
Glucagon-like peptide-1 (GLP-1) is a gut incretin hormone and new clinically available class of agents for improving insulin resistance in both animals humans with type 2 diabetes. These studies aimed to determine whether long-term treatment long-acting GLP-1 analog, exendin-4, delayed the progression Male db/db mice db/m at 8 wk age were treated exendin-4 wk, whereas control received only vehicle. Urinary albumin excretion was significantly decreased that 1 nmol/kg compared those 0.5 (P <...
Introduction Green tea catechins have diverse pharmacological effects such as anticarcinogenic and antioxidant activities. Aim To study the protective of green (−)-epicatechin (EC) against toxic streptozotocin (STZ), a selective β cell toxin, on pancreatic islets in vivo vitro. Methodology Rats were randomly divided into four groups: control, EC (30 mg/kg)–treated, STZ (60 plus (same doses; EC+STZ)–treated rats. was administered twice day for 6 days, single injection used. In EC+STZ–treated...
Glucagon-like peptide-1 (GLP-1) and its analog exendin-4 (EX) have been considered as a growth factor implicated in pancreatic islet mass increase β-cell proliferation. This study aimed to investigate the effect of EX on cyclin D1 expression, key regulator cell cycle, line INS-1. We demonstrated that significantly increased mRNA subsequently protein levels. Although induced phosphorylation Raf-1 extracellular-signal-regulated kinase (ERK), both PD98059 exogenous ERK1 had no induction by EX....
Glucagon-like peptide-1 (GLP-1) and its potent agonists have been widely studied in pancreatic islet β-cells. However, GLP-1 receptors are present many extrapancreatic tissues including macrophages, thus may diverse actions these cells. Therefore, we examined the mechanism by which exendin-4 (EX-4), a receptor agonist, inhibits lipopolysaccharide (LPS)-induced iNOS expression Raw264.7 macrophage EX-4 significantly inhibited LPS-induced protein nitrite production. Northern blot promoter...
Islet transplantation is a potential strategy to cure type 1 diabetes mellitus. However, substantial part of the islet graft becomes nonfunctional due several factors including hypoxia. precise mechanism cell damage largely unknown in hypoxic exposure pancreatic beta cells. The aim present study was investigate whether acute and severe injury could involve inducible nitric oxide synthase (iNOS)-nitric (NO) signaling cells.The rat line (INS-1) primary islets were incubated an anoxic chamber....
Background Hypoxia can occur in pancreatic islets type 2 diabetes mellitus. Pancreatic stellate cells (PSCs) are activated during hypoxia. Here we aimed to investigate whether PSCs within the islet also hypoxia, causing β-cell injury. Methods Islet and primary were isolated from Sprague Dawley rats, cultured normoxia (21% O2) or hypoxia (1% O2). The expression of α-smooth muscle actin (α-SMA), as measured by immunostaining Western blotting, was used a marker PSC activation. Conditioned media...
Influenza epidemics frequently and unpredictably break out all over the world, seriously affect breeding industry human activity. Inactivated live attenuated viruses have been used as protective vaccines but exhibit high risks for biosafety. Subunit enjoy biosafety specificity a few weak points compared to inactivated virus or vaccines, especially in low immunogenicity. In this study, we developed new subunit vaccine platform potent, adjuvant-free, multivalent vaccination. The ectodomains of...
Cytokines such as interleukin-1β (IL-1β) stimulate inducible nitric oxide synthase (iNOS) expression and overproduction leading to β-cell damage. Meanwhile, glucagon-like peptide-1 (GLP-1) its potent analog exendin-4 (EX-4) were well known for proliferation. However, the protective mechanisms of GLP-1 in β-cells exposed cytokines not fully elucidated. Therefore, effects EX-4 on IL-1β-induced iNOS gene investigated employing RINm5F β-cells. inhibited protein nitrite production. northern blot...
High doses of glucosamine have been known to induce apoptosis pancreatic beta cells. The mechanism for this phenomenon has not clearly elucidated. We aimed explore the potential mechanisms toxicity in rat insulinoma cell line INS-1 and native also investigated whether glucagon-like peptide (GLP)-1 could be protective against glucosamine. Glucosamine exhibited dose-dependent inhibition survival an increase population at sub-G1 phase. was revealed inhibit cellular glucose uptake, resulting...
Background Glucose toxicity that is caused by chronic exposure to a high glucose concentration leads islet dysfunction and induces apoptosis in pancreatic β-cells. Heme oxygenase-1 (HO-1) has been identified as an anti-apoptotic cytoprotective gene. The purpose of this study investigate whether HO-1 up-regulation when using metalloprotophyrin (cobalt protoporphyrin, CoPP) could protect β-cells from glucose-induced apoptosis. Methods Reverse transcription-polymerase chain reaction was...
Abstract Hexokinase1 (HXK1) is an Arabidopsis glucose sensor that has a variety of roles during plant growth and devlopment, including germination, flowering, senescence. HXK1 also acts as positive regulator immune responses. Previous research suggested might influence responses via to glucose. Plant are governed by two main pathways: PAMP-triggered immunity (PTI) effector-triggered (ETI). PTI involves the recognition Pathogen-Associated Molecular Patterns (PAMPs) leads increased callose...
Glucagon-like peptide-1 (GLP-1) induces several immediate early response genes such as c-fos, c-jun, and growth response-1 (Egr-1), which are involved in cell proliferation differentiation. We recently reported that exendin-4 (EX-4), a potent GLP-1 agonist, upregulated Egr-1 expression via phosphorylation of CREB, transcription factor INS-1 beta-cells. This study was designed to investigate the role another factors, serum (SRF) Yin Yang-1 (YY1), EX-4-induced expression. EX-4 significantly...
Various subtypes of enteroendocrine cells (EECs) are present in the gut epithelium. EECs and pancreatic β-cells share similar pathways differentiation during embryonic development after birth. In this study, similarities between were evaluated detail. To obtain specific EECs, cell sorting by flow cytometry was conducted from STC-1 (a heterogenous EEC line), each single cultured passaged. Five established according to hormone expression, measured quantitative RT-PCR immunostaining: L, K, I, G...