A5100685129- Pesticide Residue Analysis and Safety
- Insect and Pesticide Research
- Influenza Virus Research Studies
- Pesticide Exposure and Toxicity
- Animal Disease Management and Epidemiology
- Virology and Viral Diseases
- Pesticide and Herbicide Environmental Studies
- Phytoestrogen effects and research
- Advanced biosensing and bioanalysis techniques
- Analytical chemistry methods development
- Respiratory viral infections research
- Phytochemicals and Antioxidant Activities
- Agricultural safety and regulations
- Metabolomics and Mass Spectrometry Studies
- Natural product bioactivities and synthesis
- Animal Virus Infections Studies
- Antibiotics Pharmacokinetics and Efficacy
- Asthma and respiratory diseases
- Phytochemistry and Biological Activities
- SARS-CoV-2 and COVID-19 Research
- Fungal Plant Pathogen Control
- Viral Infections and Vectors
- Precipitation Measurement and Analysis
- Biosensors and Analytical Detection
- Digital Marketing and Social Media
U.S. National Poultry Research Center
2024-2025
Agricultural Research Service
2024-2025
Gyeongsang National University
2023-2025
Konkuk University
2014-2024
Kangwon National University
2013-2024
Seoul National University
2008-2023
Pukyong National University
2023
Konkuk University Medical Center
2014-2022
Animal and Plant Quarantine Agency
2021
Yonsei University
2020
During October 2020-January 2021, we isolated a total of 67 highly pathogenic avian influenza (HPAI) H5N8 viruses from wild birds and outbreaks in poultry South Korea. We sequenced the isolates performed phylogenetic analysis complete genome sequences to determine origin, evolution, spread patterns these viruses. Phylogenetic hemagglutinin (HA) gene showed that all belong H5 clade 2.3.4.4 subgroup B (2.3.4.4b) form two distinct genetic clusters, G1 G2. The cluster was closely related...
In this study, Lacticaseibacillus paracasei LAB47 (LAB47) and Levilactobacillus brevis WCP02 (WCP02) were selected for the fermentation of isoflavone-enriched soybean leaves (IESLs) according to their survival capability under artificial gastric acid, β-glucosidase activity, γ-aminobutyric acid (GABA) production ability, isoflavone contents. The strain ratio duration with IESLs 1:1 (per 2.5%, v/v) a time 72 h. Finally, fermented (FIESLs) compared raw steamed (RIESLs SIESLs, respectively)...
A multiresidue method for the simultaneous and rapid analysis of 360 pesticides in representative agricultural produce (brown rice, orange, spinach, potato) was developed using a modified QuEChERS procedure combined with gas chromatography–tandem mass spectrometry (GC-MS/MS). Selected reaction monitoring transition parameters (e.g., collision energy, precursor product ions) MS/MS were optimized to achieve best selectivity sensitivity wide range GC-amenable pesticides. short (20 m) microbore...
A screening method for simultaneous analysis of 379 pesticides in human serum was developed using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Electrospray ionization with positive/negative switching mode LC–MS/MS adopted, and scheduled multiple reaction monitoring each target compound established. The limit quantitation 10 ng/mL 94.5% the total pesticides, correlation coefficients calibration were ≥0.990 93.9% pesticides. For sample preparation, scaled-down QuEChERS used....
Multiresidual pesticide analysis in hair can provide useful perspectives on the relationship between pesticides and human health. To establish a rapid simultaneous analytical method using LC-MS/MS GC-MS/MS, optimization of pulverization, extraction solvent purification with dispersive SPE was performed for 300 pesticides. Hair pulverization standardized ball mill, at 30 Hz 20 min (10 twice), 3-mm diameter beads. For extraction, 0.1% formic acid acetonitrile selected, PSA d-SPE chosen...
The matrix effect refers to the change in analytical signal caused by which sample is contained, as well impurities that are co-eluted with target analyte. In crop analysis using LC–MS/MS, can affect quantification results. Chinese chives likely exhibit a strong when co-extracted bifenthrin and butachlor due presence of phytochemicals chlorophyll. A novel method was developed reduce effects negligible level chives. established had limit quantitation 0.005 mg/kg correlation coefficients...
Airborne pathogens causing infectious diseases are often highly transmittable between humans. Therefore, an airborne pathogen-monitoring system capable of on-site detection and identification would aid tremendously in preventing controlling the early stages pathogen spread. Here, we describe integrated sampling/monitoring platform for real-time viruses. We used MS2 bacteriophage avian influenza virus (AIV) H1N1 to evaluate bioaerosol sampling performance platform. Our results show that,...
Cinnamon is a natural spice with wide range of pharmacological functions, including anti-microbial, antioxidant, and anti-tumor activities. The aim this study to investigate the effects cinnamaldehyde-rich cinnamon extract (CRCE) on colorectal cancer cell lines HCT 116 HT-29. gas chromatography mass spectrometry analysis lipophilic revealed dominance trans-cinnamaldehyde. Cells treated CRCE (10-60 µg/mL) showed significantly decreased viability in time- dose-dependent manner. We also...
The outbreak of clade 2.3.4.4b H5 highly pathogenic avian influenza (HPAI) in North America that started 2021 has increased interest applying vaccination as a strategy to help control and prevent the disease poultry. Two commercially available vaccines based on recombinant herpes virus turkeys (rHVT) vector were tested against recent American HPAI isolate: A/turkey/Indiana/22-003707-003/2022 H5N1 specific pathogen free white leghorn (WL) chickens commercial broiler chickens. One rHVT-H5...
Multiresidual pesticide determination in a biological sample is essential for an immediate decision and response related to various intoxications. A rapid simultaneous analytical method 260 pesticides human urine was developed validated using liquid chromatography-tandem mass spectrometry (LC-MS/MS). High speed positive/negative switching electrospray ionization (ESI) mode used, scheduled multiple reaction monitoring (MRM) optimized. Three versions of scaled-down QuEChERS procedures were...
In this study, a signal-amplifiable nanoprobe-based chemiluminescent lateral flow immunoassay (CL-LFA) was developed to detect avian influenza viruses (AIV) and other contagious fatal viral avian-origin diseases worldwide. Signal-amplifiable nanoprobes are capable of size-selective immobilization antibodies (binding receptors) enzymes (signal transducers) on sensitive paper-based sensor platforms. Particle structure designs conjugation pathways conducive for antigen accessibility maximum...
The H5 subtype highly pathogenic avian influenza virus (HPAIV) has been introduced to South Korea every 2 or 3 years via wild migratory waterfowls, causing devastating damages the poultry industry. Although most and economic losses by HPAIV are focused on chicken layers, domestic ducks known play a major role in farm-to-farm transmission. However, vaccine studies have performed with oil-emulsion inactivated vaccines. In this study, we developed live recombinant Newcastle disease...
A Newcastle disease virus (NDV)-vectored vaccine expressing clade 2.3.4.4b H5 Hemagglutinin was developed and assessed for efficacy against H5N1 highly pathogenic avian influenza (HPAI) in specific pathogen-free (SPF) chickens, broilers, domestic ducks. In SPF the live recombinant NDV-vectored vaccine, rK148/22-H5, achieved complete survival HPAI NDV challenges significantly reduced viral shedding. Notably, rK148/22-H5 conferred good clinical protection broilers despite presence of...
The hypothesis of autoimmune involvement in asthma has received much recent interest. Autoantibodies, such as anti-cytokeratin (CK) 18, anti-CK19, and anti- α -enolase antibodies, react with self-antigens are found at high levels the sera patients severe (SA). However, mechanisms underlying autoantibody production SA have not been fully determined. present study was conducted to demonstrate that neutrophil extracellular DNA traps (NETs), cytotoxic molecules released from neutrophils, a key...
Estimating the AAR (Areal Average Rainfall) is an essential process when determining accurate amount of available water resources and building input data which integral to Rainfall-Runoff Analysis. To estimate AAR, using rain gauge networks that are spatially well distributed ideal. In this study, spatial characteristics for five major river basins in South Korea considered influence distribution has on estimation evaluated. For purpose, error calculated two cases. The first case (Analysis...