A5080488007- Plant Pathogens and Resistance
- Potato Plant Research
- Plant Disease Resistance and Genetics
- Plant nutrient uptake and metabolism
- Chromosomal and Genetic Variations
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Genomics and Phylogenetic Studies
- Plant responses to elevated CO2
- Genomics and Chromatin Dynamics
- Plant Genetic and Mutation Studies
- Seed Germination and Physiology
- Plant Molecular Biology Research
- Botanical Research and Chemistry
- Plant Pathogenic Bacteria Studies
- Plant Gene Expression Analysis
- Plant and fungal interactions
- Gene expression and cancer classification
- RNA modifications and cancer
- Edible Oils Quality and Analysis
- Molecular Biology Techniques and Applications
- Lipid metabolism and biosynthesis
- Nitrogen and Sulfur Effects on Brassica
- RNA regulation and disease
- Plant Stress Responses and Tolerance
Agriculture and Agri-Food Canada
2016-2025
Fredericton Research and Development Centre
2012-2024
Natural Resources Canada
2004-2009
Canadian Forest Service
2005-2009
Potato Research Institute
2009
Ottawa Regional Cancer Foundation
2003
University of Ottawa
1994-2003
University of Toronto
1989
Cultivated potato is a clonally propagated autotetraploid species with highly heterogeneous genome. Phased assemblies of six cultivars including two chromosome-scale phased genome revealed extensive allelic diversity, altered coding and transcript sequences, preferential allele expression, structural variation that collectively result in complex transcriptome predicted proteome, which are distributed across the homologous chromosomes. Wild contribute to diversity tetraploid cultivars,...
Potato (Solanum sp., family Solanaceae) is the most important noncereal food crop globally. It has over 100 wild relatives in Solanum section Petota, which features species with both sexual and asexual reproduction varying ploidy levels. A pangenome of Petota composed 296 accessions was constructed including diploids polyploids compared via presence/absence variation (PAV). The core (genes shared by at least 97% accessions) shell genomes (shared 3 to 97%) are enriched basic molecular...
Abstract Nitrogen (N) is the most important nutrient for growth of potato ( Solanum tuberosum L.). Foliar gene expression in plants with and without N supplementation at 180 kg ha −1 was compared mid-season. Genes consistent differences foliar due to over three cultivars two developmental time points were examined. In total, thirty genes found be over-expressed nine under-expressed supplemented N. Functional relationships between found. The main metabolic pathway represented among...
Abstract Key message Twelve potato accessions were selected to represent two principal views on taxonomy. The genomes sequenced and analyzed for structural variation (copy number variation) against three published genomes. common ( Solanum tuberosum L.) is an important staple crop with a highly heterozygous complex tetraploid genome. other taxa of cultivated contain varying ploidy levels (2 X –5 ), variations are in the these species, likely contributing diversification or agronomic traits...
Abstract Genome assembly of polyploid plant genomes is a laborious task as they contain more than two copies the genome, are often highly heterozygous with high level repetitive DNA. Next Generation genome sequencing data representing one Chilean and five Peruvian potato ( Solanum spp .) landrace was used to construct assemblies comprising taxa. Third (Linked Long-read data) improve for genomes. Native landraces valuable genetic resources traits such disease pest resistance, environmental...
The Colorado potato beetle Leptinotarsa decemlineata (Say) (CPB) is a coleopteran herbivore that feeds on the foliage Solanum species, in particular, potato. Six resistant wild species were identified, and two of these had low levels glycoalkaloids. Comparative analysis untargeted metabolite profiles using UPLC-qTOF-MS was done to find metabolites shared between but not with tuberosum (L.) identify resistance-related metabolites. It found only S. produced triose glycoalkaloids solanine...
Steroidal glycoalkaloids (SGAs) are major secondary metabolites constitutively produced in cultivated potato Solanum tuberosum, and α-solanine α-chaconine the most abundant SGAs. SGAs toxic to humans at high levels but their role plant protection against pests pathogens is yet be established. In this study, of were reduced by RNA interference (RNAi)-mediated silencing GLYCOALKALOID METABOLISM 4 (GAME4)-a gene encoding cytochrome P450, involved an oxidation step conversion cholesterol SGA...
Potatoes usually have the tuber bud end dominance in growth during bulking and sprouting, likely using carbohydrates from stem end. We hypothesized that coordination carbohydrate metabolism gene expression is different between sprouting of After comparing growing tubers at harvest a green vine stage sprouts just started to emerge after storage room temperature, we found following: (1) Dry matter content was higher than both stages. (2) The starch granule size larger (3) had for synthesis but...
The chloroplasts are a crucial part of photosynthesizing plant cells and extensively utilized in phylogenetic studies mainly due to their maternal inheritance. Characterization analysis complete plastome sequences is necessary understand diversity evolutionary relationships. Here, panel thirteen plastomes from various potato taxa presented. Though they highly similar with respect gene order content, there also great extent SNPs InDels between them, one the Solanum bukasovii (BUK2) having...
Cells of the nonfusing muscle cell line BC3H1 stop proliferating and express a family muscle-specific proteins when FBS concentration is reduced from 20 to 0.5% (Munson, R., K.L. Caldwell, L. Glaser. 1982. J. Cell Biol. 92:350-356). Several growth factors have been shown block differentiation in this line. To begin investigate potential role G signal transducing pathways these receptors, we examined effects cholera toxin (CT) pertussis (PT) on proliferation cells. PT specifically ADP...
Cultivated potato is a vegetatively propagated crop, and most varieties are autotetraploid with high levels of heterozygosity. Reducing the ploidy breeding at diploid level can increase efficiency for genetic improvement including greater ease introgression wild relatives more efficient use genomics markers in selection. More recently, selfing diploids generation inbred lines F1 hybrid has had lot attention potato. The current study provides resources nine legacy non-inbred adapted clones...
MYB and bHLH are large transcription factor families with largely uncharacterized biological functions. The patterns of expression 42 58 genes were examined in potato clones that demonstrated to have variation anthocyanin intensity common scab resistance assess their possible involvement regulating these traits. control biosynthetic enzymes regulation was also evaluated. dihydroflavonol 4-reductase (DFR) gene, a key enzyme the pathway, had highest correlation gene (quantified as levels...