A5100411275- interferon and immune responses
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Cytomegalovirus and herpesvirus research
- Advanced biosensing and bioanalysis techniques
- Immune Response and Inflammation
- DNA and Nucleic Acid Chemistry
- Gene expression and cancer classification
- Biochemical and Molecular Research
- Antimicrobial Peptides and Activities
- Immunotherapy and Immune Responses
- RNA Research and Splicing
- Cancer Mechanisms and Therapy
- Entomopathogenic Microorganisms in Pest Control
- Traditional and Medicinal Uses of Annonaceae
- Adenosine and Purinergic Signaling
- Catalytic Alkyne Reactions
- Bacterial biofilms and quorum sensing
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Studies on Chitinases and Chitosanases
- Virus-based gene therapy research
- Invertebrate Immune Response Mechanisms
- Autophagy in Disease and Therapy
- Ginseng Biological Effects and Applications
- Herpesvirus Infections and Treatments
China Medical University
2024
First Hospital of China Medical University
2024
Harvard University
2023
Dana-Farber Cancer Institute
2023
Parker Institute for Cancer Immunotherapy
2023
Ningbo University
2023
University of California, San Diego
2016-2022
Fudan University
2022
Huashan Hospital
2022
University of California System
2021
Cyclic GMP-AMP synthase (cGAS) is an enzyme in human cells that controls immune response to cytosolic DNA. Upon binding DNA, cGAS synthesizes a nucleotide signal 2′3′-cGAMP activates STING-dependent downstream immunity. Here, we discover cGAS-like receptors (cGLRs) constitute major family of pattern recognition innate Building on recent analysis Drosophila, identify >3,000 cGLRs present nearly all metazoan phyla. A forward biochemical screening 150 animal reveals conserved mechanism...
Lactobacillus strains fermentation of broccoli as a good source bioactive peptides has not been fully elucidated. In this work, the peptide composition fermented by L. plantarum A3 and rhamnosus ATCC7469 was analyzed peptidomics to study protein digestion patterns after different strains. Results showed that water-soluble proteins such rubisco were abundant sources peptides, which triggered sustained release main target hydrolysis. addition, 17 novel anti-inflammatory identified virtual...
Abstract An enzyme‐mediated approach for the assembly of singly modified RNA constructs in which specific G residues are replaced with th G, an emissive isomorphic surrogate, is reported. Transcription presence and native nucleoside triphosphates enforces initiation unnatural analogue, yielding 5′‐end transcripts that can be mono‐phosphorylated ligated to provide longer site‐specifically constructs. The scope this unprecedented enzymatic non‐canonical purine‐containing RNAs explored via...
Drosophila melanogaster is widely used as a model system to study innate immunity and signaling pathways related immunity, including the Toll pathway. Although this pathway well studied, precise mechanisms of posttranscriptional regulation key components by microRNAs (miRNAs) remain obscure. In study, we an in silico strategy combination with Gal80ts-Gal4 driver identify microRNA-958 (miR-958) candidate regulating miRNA We report that overexpression miR-958 significantly reduces expression...
We evaluated the effect of Tween 80 permeabilization on ginsenoside secretion in Panax ginseng hairy roots. (1.2%, w/v) had no significant root vitality. After a 25-day treatment with 80, approximately 76% total ginsenosides was released into surrounding medium. In case control, secreted medium were negligible. Furthermore, when compared level enhanced by threefold under treatment. Additionally, typical monomers including Rb1 , Rg1 and Re analyzed, indicating that most them Moreover, it...
Ribosome-inactivating proteins, a family of highly cytotoxic interfere with protein synthesis by depurinating specific adenosine residue within the conserved α-sarcin/ricin loop eukaryotic ribosomal RNA. Besides being biological warfare agents, certain RIPs have been promoted as potential therapeutic tools. Monitoring their deglycosylation activity and inhibition in real time remained, however, elusive. Herein, we describe enzymatic preparation utility consensus RIP hairpin substrates which...
Guanine deaminase (GDA) deaminates guanine to xanthine. Despite its significance, the study of human GDA remains limited compared other metabolic deaminases. As a result, substrate and inhibitor repertoire are limited, effective real-time activity, inhibitory, discovery assays missing. Herein, we explore two emissive heterocyclic cores, based on thieno[3,4-d]pyrimidine (thN) isothiazole[4,3-d]pyrimidine (tzN), as surrogate substrates. We demonstrate that, unlike thieno analog, thGN,...
cGAS (cyclic GMP-AMP synthase) is an enzyme in human cells that controls immune response to cytosolic DNA. Upon binding DNA, synthesizes a nucleotide signal 2'3'-cGAMP activates the protein STING and downstream immunity. Here we discover cGAS-like receptors (cGLRs) constitute major family of pattern recognition animal innate Building on recent analysis
Mitochondrial dysfunction is a pivotal event contributing to the development of ageing-related kidney disorders. Lon protease 1 (LONP1) has been reported be responsible for renal fibrosis; however, underlying mechanism(s) LONP1-driven ageing with respect mitochondrial disturbances remains further explored. The level LONP1 was tested in kidneys aged humans and mice. Renal fibrosis quality control were confirmed Effects silencing or overexpression on In addition, N6-methyladenosine (m6A)...
Colon cancer is one of the most common malignancies causing majority cancer-related deaths worldwide. The tripartite motif family protein 72 (TRIM72), also known as mitsugumin 53, acts an E3 ubiquitin ligase. TRIM72 involved in insulin resistance and metabolic syndrome, which are risk factors colon cancer. However, correlation between remains unknown. In present study, we explored expression profile tissues diagnostic value serum receiver operating characteristic (ROC) curves were applied...
<h3>Abstract</h3> Neuropsychological studies indicate that healthy aging is associated with a decline of inhibitory control attentional and behavioral systems. A widely accepted measure the antisaccade task requires both inhibition reflexive saccadic response toward visual target initiation voluntary eye movement in opposite direction. To better understand nature age-related differences control, we evaluated performance 78 younger (20–35 years) older (60–80 participants. In order to provide...
Apart from its vital function as a redox cofactor, nicotinamide adenine dinucleotide (NAD+ ) has emerged crucial substrate for NAD+ -consuming enzymes, including poly(ADP-ribosyl)transferase 1 (PARP1) and CD38/CD157. Their association with severe diseases, such cancer, Alzheimer's disease, depressions, necessitates the development of new analytical tools based on traceable surrogates. Here, synthesis, photophysics biochemical utilization an emissive, thieno[3,4-d]pyrimidine-based surrogate,...
Abstract Bacterial cyclic dinucleotides (CDNs) play important roles in regulating biofilm formation, motility and virulence. In eukaryotic cells, theses bacterial CDNs are recognized as pathogen‐associated molecular patterns (PAMPs) trigger an innate immune response. We report the photophysical analyses of a novel group enzymatically synthesized emissive CDN analogues comprised two families isomorphic ribonucleotides. The highly favorable features analogues, when compared to their...
Abstract An enzyme‐mediated approach for the assembly of singly modified RNA constructs in which specific G residues are replaced with th G, an emissive isomorphic surrogate, is reported. Transcription presence and native nucleoside triphosphates enforces initiation unnatural analogue, yielding 5′‐end transcripts that can be mono‐phosphorylated ligated to provide longer site‐specifically constructs. The scope this unprecedented enzymatic non‐canonical purine‐containing RNAs explored via...
A theoretical dynamic kinetic model was derived and a series of experiments were carried out using low-density microarrays in various concentrations spotting probe ([P]) labeling target ([T]). It has been shown that determined the signal intensity together. At certain range DNA concentration, proportion to [P]. higher concentrations, there decrease hybridization intensity, especially cDNA microarrays. Since microarray constructed on solid surface, steric hindrance, which is induced by...
Abstract Cyclic dinucleotides (CDNs) trigger the innate immune response in eukaryotic cells through stimulator of interferon genes (STING) signaling pathway. To decipher this complex cellular process, a better correlation between structure and downstream function is required. Herein, we report design immunostimulatory effect novel group c‐di‐GMP analogues. By employing an “atomic mutagenesis” strategy, changing one atom at time, class gradually modified CDNs was prepared. These analogues...
Myocardial hypertrophy is a pathological thickening of the myocardium, leading to various ailments, such as myocardial infarction and heart failure. RBM38 critical in modulating mRNA translation for multiple protective activities p53 tumor repressor p21 kinase cell cycle inhibitors. Liver X receptors (LXR-α) agonists reduce cellular initiated by hypertrophic stimuli lipopolysaccharides Ang II. This research investigates possible cooperation between LXR-α mechanisms hypertrophy. H9C2 cells...
Cytidine deaminase effectively deaminates three emissive fused cytidine analogues and the reactions can be monitored in real time by fluorescence.
The core function in human resource management is compensation management, and it also the focus problem which brings dispute conflict between employers employees. In this passage we will discuss some important factors of pay effectiveness.
<title>Abstract</title> <bold>Purpose: </bold>Hypoxia-inducible factor proline hydroxylase inhibitors (HIF-PHIs) may affect the STING-TBK1-IRF3 pathway by targeting polarization of macrophages to prevent progression Renal interstitial fibrosis (RIF). However, mechanism action remains unclear. <bold>Methods:</bold> A total 60 patients were enrolled in clinical study with 30 each for control and RIF group. Kidney tissue blood samples collected expression M1-type macrophage markers (MHC-II,...
Abstract Mathematical models of viral dynamics in vivo provide incredible insights into the mechanisms for nonlinear interaction between virus and host cell populations, drug resistance, way to eliminate infection from individual patients by treatment. The integration these mathematical with high-throughput genetic genomic data within a statistical framework will raise hope effective treatment infections HIV through developing potent antiviral drugs based on patients’ makeup. In this opinion...