A5088277782- Cellular Mechanics and Interactions
- Cell Image Analysis Techniques
- Cancer, Stress, Anesthesia, and Immune Response
- IL-33, ST2, and ILC Pathways
- Advanced Fluorescence Microscopy Techniques
- Cell Adhesion Molecules Research
- Inflammasome and immune disorders
- Helicobacter pylori-related gastroenterology studies
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Anesthesia and Neurotoxicity Research
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Macrophage Migration Inhibitory Factor
- Muscle Physiology and Disorders
- Vasculitis and related conditions
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Morphological variations and asymmetry
- Cardiomyopathy and Myosin Studies
- RNA regulation and disease
- Vagus Nerve Stimulation Research
- Electrospun Nanofibers in Biomedical Applications
- Neonatal and fetal brain pathology
- Fetal and Pediatric Neurological Disorders
- Eosinophilic Esophagitis
Monash University
2015-2023
Good Shepherd Rehabilitation
2023
Community Care
2023
Hudson Institute of Medical Research
2018-2022
Monash Health
2022
Australian Regenerative Medicine Institute
2020
University of North Carolina at Chapel Hill
2011-2014
Howard Hughes Medical Institute
2011
Children's Hospital at Westmead
2008-2010
The University of Sydney
2008-2010
Abstract Macrophage migration inhibitory factor (MIF) exerts multiple effects on immune cells, as well having functions outside the system. MIF can promote inflammation through induction of other cytokines, including TNF, IL-6, and IL-1 family cytokines. Here, we show that inhibition regulates release IL-1α, IL-1β, IL-18, not by affecting transcription or translation these but via activation NLRP3 inflammasome. is required for interaction between intermediate filament protein vimentin, which...
Chronic stress accelerates metastasis - the main cause of death in cancer patients through activation β-adrenoceptors (βARs). We have previously shown that β2AR signaling MDA-MB-231(HM) breast cells, facilitates invadopodia formation and invasion vitro. However, tumor microenvironment where many stromal cells also express βAR, role is unclear. Therefore, to investigate contribution vivo, we used RNA interference generate are deficient β2AR. knockdown reduced proportion with a...
For efficient metastatic dissemination, tumor cells form invadopodia to degrade and move through three-dimensional extracellular matrix. However, little is known about the conditions that favor formation. Here, we investigated effect of β-adrenoceptor signaling - which allows respond stress neurotransmitters on formation examined cell invasion. To characterize molecular cellular mechanisms β-adrenergic invasive properties breast cancer cells, used functional assays quantify evaluate invasion...
The balance of transition between distinct adhesion types contributes to the regulation mesenchymal cell migration, and characteristic association adhesions with actin filaments led us question role filament-associating proteins in adhesive states. Tropomyosin isoform imparts filament structures, we have thus investigated for tropomyosins determining formation structures. Using combinations overexpression, knockdown, knockout approaches, establish that Tm5NM1 preferentially stabilizes focal...
Abstract dsRNA is a common by-product of viral replication and acts as potent trigger antiviral immunity. SIDT1 SIDT2 are closely related members the SID-1 transmembrane family. functions transporter required to traffic internalized from endocytic compartments into cytosol for innate immune activation, but role in transport response infection unclear. In this study, we show that Sidt1 expression upregulated type I IFN exposure interacts with SIDT2. Moreover, similar SIDT2, localizes...
Abstract The interleukin-1 family members, IL-1β and IL-18, are processed into their biologically active forms by multi-protein complexes, known as inflammasomes. Although the inflammasome pathways that mediate processing in myeloid cells have been defined, those involved IL-18 processing, particularly non-myeloid cells, still not well understood. Here we report host defence molecule NOD1 regulates mouse epithelial response to mucosal pathogen, Helicobacter pylori . Specifically, mediates...
Helicobacter pylori (H. pylori) causes chronic inflammation which is a key precursor to gastric carcinogenesis. It has been suggested that H. may limit this immunopathology by inducing the production of interleukin 33 (IL-33) in epithelial cells, thus promoting T helper 2 immune responses. The molecular mechanism underlying IL-33 response infection, however, remains unknown. In study, we demonstrate activates signalling via pathogen recognition molecule Nucleotide-Binding Oligomerisation...
Abstract Mitochondria and mitochondrial dynamics play vital roles in health disease. With the intricate nanometer-scale structure rapid of mitochondria, super-resolution microscopy techniques possess great un-tapped potential to significantly contribute understanding biology kinetics. Here we present a novel probe (MitoRed AIE) suitable for live imaging single particle tracking (SPT), together with multi-dimensional data analysis approach assess local (membrane) fluidity. The MitoRed AIE...
Antenatal brain development during the final trimester of human pregnancy is a time when mature neurons become increasingly complex in morphology, through axonal and dendritic outgrowth, branching, synaptogenesis, together with myelin production. Characterizing neuronal morphological over interest to developmental neuroscience provides framework measure gray matter pathology compromise. Neuronal microstructure can be assessed Golgi staining, which selectively stains small percentage (1-3%)...
Multiple cell types form specialized protein complexes that are used by the to actively degrade surrounding extracellular matrix. These structures called podosomes or invadopodia and collectively referred as invadosomes. Due their potential importance in both healthy physiology well pathological conditions such cancer, characterization of these has been increasing interest. Following early descriptions invadopodia, assays were developed which labelled matrix underneath metastatic cancer...
Glomerular cell death is a pathological feature of myeloperoxidase anti neutrophil cytoplasmic antibody associated vasculitis (MPO-AAV). Extracellular deoxyribonucleic acid (ecDNA) released during different forms including apoptosis, necrosis, necroptosis, extracellular traps (NETs) and pyroptosis. Measurement this time consuming with several biomarkers required to identify the biochemical death. ecDNA generally conducted in serum urine as surrogate for renal damage, not actual target organ...
Glomerular cell death is a pathological feature of myeloperoxidase anti neutrophil cytoplasmic antibody associated vasculitis (MPO-AAV). Extracellular deoxyribonucleic acid (ecDNA) released during different forms including apoptosis, necrosis, necroptosis, extracellular traps (NETs) and pyroptosis. Measurement this time consuming with several biomarkers required to identify the biochemical death. ecDNA generally conducted in serum urine as surrogate for renal damage, not actual target organ...
Multiple cell types form specialized protein complexes, podosomes or invadopodia and collectively referred to as invadosomes, which are used by the actively degrade surrounding extracellular matrix. Due their potential importance in both healthy physiology well pathological conditions such cancer, characterization of these structures has been increasing interest. Following early descriptions invadopodia, assays were developed labelled matrix underneath metastatic cancer cells allowing for...
Multiple cell types form specialized protein complexes, podosomes or invadopodia and collectively referred to as invadosomes, which are used by the actively degrade surrounding extracellular matrix. Due their potential importance in both healthy physiology well pathological conditions such cancer, characterization of these structures has been increasing interest. Following early descriptions invadopodia, assays were developed labelled matrix underneath metastatic cancer cells allowing for...
Abstract Chronic stress accelerates breast cancer metastasis through beta-adrenergic signaling and recent clinical studies found that beta-blockade reduced in women with cancer. However, the specific receptor subtype(s) which stress-responsive neurotransmitters mediates effects on remain unknown. To identify transmits to primary mammary tumors, we used bioluminescence imaging longitudinally quantify effect of beta-blockers selectively inhibit beta1 vs. beta 2 adrenergic receptors an...