A5083292750- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Retinal Development and Disorders
- stochastic dynamics and bifurcation
- Receptor Mechanisms and Signaling
- Axon Guidance and Neuronal Signaling
- Mitochondrial Function and Pathology
- Cell Image Analysis Techniques
- Genetics and Neurodevelopmental Disorders
- Electrochemical Analysis and Applications
- Neuroendocrine regulation and behavior
- Advanced Memory and Neural Computing
- Marine animal studies overview
- Photoreceptor and optogenetics research
- Animal Vocal Communication and Behavior
- Congenital heart defects research
- Autism Spectrum Disorder Research
- Hearing, Cochlea, Tinnitus, Genetics
Erasmus MC
2016-2024
Institut de la Vision
2020-2021
Inserm
2020-2021
Sorbonne Université
2020-2021
Centre National de la Recherche Scientifique
2020-2021
Erasmus University Rotterdam
2020
Amsterdam Neuroscience
2013
Brain function and behavior undergo significant plasticity refinement, particularly during specific critical sensitive periods. In autistic intellectual disability (ID) neurodevelopmental disorders (NDDs) their corresponding genetic mouse models, impairments in many neuronal behavioral phenotypes are temporally regulated some cases, transient. However, the links between neurobiological mechanisms governing typically normal brain development (referred to also as "neurotypical" development)...
Significance During high-frequency firing, the shape of a presynaptic action potential (AP) can alter, thereby changing neurotransmitter release. In this paper, we describe how giant terminal in brainstem newborn rats called calyx Held fire vivo at high frequencies without substantial AP depression. The underlying mechanism was found to be presence sodium channels that recover rapidly from depression combination with close match between is attained following an maximizes stability....
Axonal arbors in many neuronal networks are exuberant early during development and become refined by activity-dependent competitive mechanisms. Theoretical work proposed non-competitive interactions between co-active axons to co-stabilize their connections, but the demonstration of such is lacking. Here, we provide experimental evidence that reducing cyclic AMP (cAMP) signaling a subset retinal ganglion cells favors elimination thalamic projections from neighboring neurons, pointing...
Neurons in the medial nucleus of trapezoid body anaesthetized rats postnatal day (P)2-6 showed burst firing with a preferred interval about 100 ms, which was stable, and second 5-30 shortened during development. In 3 out 132 cases, evidence for presence two large inputs found. vivo whole-cell recordings revealed that excitability principal neuron size its largest synaptic were developmentally matched. At P2-4, action potentials triggered by barrages small events summated to plateau...
During development the giant, auditory calyx of Held forms a one-to-one connection with principal neuron medial nucleus trapezoid body. While anatomical studies described that most target cells are temporarily contacted by multiple calyces, multi-calyceal innervation was only sporadically observed in vivo recordings, suggesting structure-function discrepancy. We correlated synaptic strength inputs, identified post hoc labelling recorded and terminals containing vesicular glutamate...
The action potential of most vertebrate neurons initiates in the axon initial segment (AIS) and is then transmitted to soma where it regenerated by somatodendritic sodium channels. For successful transmission, AIS must produce a strong axial current, so as depolarize threshold for somatic regeneration. Theoretically, this current depends on geometry Na+ conductance density. We measured mouse retinal ganglion cells using whole cell recordings with post hoc labeling. found that large, implying...
Having two ears enables us to localize sound sources by exploiting interaural time differences (ITDs) in arrival. Principal neurons of the medial superior olive (MSO) are sensitive ITD, and each MSO neuron responds optimally a best ITD (bITD). In many cells, especially those tuned low frequencies, these bITDs correspond ITDs for which contralateral ear leads, often larger than ecologically relevant range, defined ratio distance speed sound. Using vivo recordings gerbils, we found that...
At synapses, the pre- and postsynaptic cells get so close that currents entering cleft do not flow exclusively along its conductance, gcl. A prominent example is found in calyx of Held synapse medial nucleus trapezoid body (MNTB), where presynaptic action potential can be recorded cell form a prespike. Here, we developed theoretical framework for ephaptic coupling via synaptic cleft, tested predictions using MNTB prespike voltage-clamp. The shape predicted to resemble either first or second...
Axonal arbors in many neuronal networks are exuberant early during development and become refined by activity-dependent competitive mechanisms. Theoretical work proposed non-competitive interactions between co-active axons to co-stabilize their connections, but the demonstration of such is lacking. Here we provide experimental evidence that reducing cAMP signaling a subset retinal ganglion cells favors elimination thalamic projections from neighboring neurons, pointing cAMP-dependent...
Abstract The action potential of most vertebrate neurons initiates in the axon initial segment (AIS), and is then transmitted to soma where it regenerated by somatodendritic sodium channels. For successful transmission, AIS must produce a strong axial current, so as depolarize threshold for somatic regeneration. Theoretically, this current depends on geometry Na + conductance density. We measured mouse RGCs using whole-cell recordings with post-hoc labeling. found that large, implying high...
The calyx of Held synapse is a giant axosomatic in the brainstem that functions as fast and reliable auditory relay. Because its large size, presynaptic action potential can be picked up recording postsynaptic cell. This so-called prespike caused by ephaptic coupling membrane currents via synaptic cleft. We constructed an electric model estimated values for leak conductance cleft, capacitance release face calcium current density based on simultaneous pre- voltage clamp recordings. These...
Abstract In adult rodents, a principal neuron in the medial nucleus of trapezoid (MNTB) is generally contacted by single, giant axosomatic terminal called calyx Held. How this one-on-one relation established still unknown, but anatomical evidence suggests that during development neurons are innervated multiple calyces, which may indicate calyceal competition. However, vivo electrophysiological recordings from indicated only single strong synaptic connection forms per cell. To test whether...
Abstract At synapses, the pre- and postsynaptic cell get so close that currents entering cleft do not flow exclusively along its conductance, g cl . A prominent example is found in calyx of Held synapse medial nucleus trapezoid body (MNTB), where presynaptic action potential can be recorded form a prespike. Here, we developed theoretical framework for ephaptic coupling via synaptic cleft, tested predictions using MNTB prespike voltage-clamp. The shape predicted to resemble either first or...