A5102923616- Enzyme Production and Characterization
- Peptidase Inhibition and Analysis
- Enzyme Catalysis and Immobilization
- Histone Deacetylase Inhibitors Research
- Biofuel production and bioconversion
- Microbial Metabolites in Food Biotechnology
- Particle Accelerators and Free-Electron Lasers
- Protein Hydrolysis and Bioactive Peptides
- Geochemistry and Elemental Analysis
- PARP inhibition in cancer therapy
- Protein Degradation and Inhibitors
- Radioactive contamination and transfer
- Carbohydrate Chemistry and Synthesis
- Phytase and its Applications
- Glycosylation and Glycoproteins Research
- Protein purification and stability
- Studies on Chitinases and Chitosanases
- Cancer, Hypoxia, and Metabolism
- Radioactivity and Radon Measurements
- Laser Design and Applications
- Geology and Paleoclimatology Research
- Particle accelerators and beam dynamics
- Gyrotron and Vacuum Electronics Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Enzyme Structure and Function
Karlsruhe Institute of Technology
2021
CrystalGenomics (South Korea)
2012-2020
Korea Food Research Institute
2014-2016
Korea Atomic Energy Research Institute
2005-2010
Seoul National University
1998-2008
Incheon National University
2007
Korea Basic Science Institute
2007
Max Planck Society
2002
National Oceanography Centre
1998
Histone deacetylase inhibitors (HDACis) are well-known epigenetic regulators with therapeutic potential in various diseases. Recent studies have shown that HDACis involved immune-mediated anti-cancer effects and may modulate the activity of immunotherapy agents. CG-745, a histone inhibitor, has pancreatic cancer, colorectal non-small cell lung cancer. However, exact role CG-745 within immune system is largely unknown. In this study, we induces microenvironment changes promoting effect...
Abstract Pancreatic cancer is predominantly lethal, and primarily treated using gemcitabine, with increasing resistance. Therefore, novel agents that increase tumor sensitivity to gemcitabine are needed. Histone deacetylase (HDAC) inhibitors emerging therapeutic agents, since HDAC plays an important role in initiation progression. We evaluated the antitumor effect of a inhibitor, CG200745, combined gemcitabine/erlotinib on pancreatic cells gemcitabine-resistant cells. Three cancer-cell lines...
A gene encoding a maltogenic amylase of Bacillus stearothermophilus ET1 was cloned and expressed in Escherichia coli . DNA sequence analysis indicated that the could encode 69 627‐Da protein containing 590 amino acids. The predicted acid enzyme shared 47−70 % identity with sequences from licheniformis , neopullulanase B. cyclodextrin hydrolase (CDase) I‐5 an alkalophilic strain. In addition to starch, pullulan cyclodextrin, hydrolyze isopanose, but not panose, glucose maltose. Maltogenic...
ABSTRACT The thermostability of maltogenic amylase from Thermus sp. strain IM6501 (ThMA) was improved greatly by random mutagenesis using DNA shuffling. Four rounds shuffling and subsequent recombination the mutations produced highly thermostable mutant enzyme ThMA-DM, which had a total seven individual mutations. amino acid substitutions in ThMA-DM were identified as R26Q, S169N, I333V, M375T, A398V, Q411L, P453L. optimal reaction temperature recombinant 75°C, 15°C higher than that...
Tubulointerstitial fibrosis is a common feature of kidney disease. Histone deacetylase (HDAC) inhibitors have been reported to attenuate renal progression. Here, we investigated the effect CG200745, novel HDAC inhibitor, on development in mouse model unilateral ureteral obstruction (UUO). To examine effects CG200745 UUO, C57BL/6 J male mice were divided into three groups: control, and (30 mg/kg/day)-treated UUO groups. CG 200745 was administered through drinking water for 1 week. Human...
Gastric cancer is the fifth most common and it still needs to identify novel actionable targets bring clinical benefits. Here we explored efficacy of nesuparib, a dual inhibitor for poly-ADP-ribosyl transferase 1/2 (PARP 1/2) tankyrase (TNKS in vitro vivo models using BRCA wild type gastric cell lines. In addition PARP inhibition, nesuparib selectively inhibits TNKS 1 2 enzymatic activities; member family, poly-ribosylates target proteins that are involved Wnt/β-catenin Hippo signaling...
TPS4227 Background: Pancreatic ductal adenocarcinoma (PDAC) is a leading cause of cancer deaths, with most cases diagnosed at advanced stages. Current maintenance therapy the PARP inhibitor, olaparib, benefits only patients germline BRCA1/2 mutations (Approximately 5-7 % PDAC cases). However, homologous recombination deficiency (HRD)-related occur in ~15% PDACs, potentially expanding utility inhibitors. Nesuparib, next-generation also targets tankyrase, disrupting WNT and Hippo signaling...
A treX in the trehalose biosynthesis gene cluster of Sulfolobus solfataricus ATCC 35092 has been reported to produce TreX, which hydrolyzes α-1,6-branch portion amylopectin and glycogen. TreX exhibited 4-α-d-glucan transferase activity, catalyzing transfer α-1,4-glucan oligosaccharides from one molecule another case linear maltooligosaccharides (G3–G7), it produced cyclic glucans amylose like 4-α-glucanotransferase. These results suggest that is a novel isoamylase possessing properties
A bread-baking process was developed using a potential novel enzyme, cyclodextrin glucanotransferase[3−18] (CGTase[3−18]), that had previously been engineered to have enhanced hydrolyzing activity with little (CD) formation toward starch. CGTase[3−18] primarily manipulated be displayed on the cell surface of Saccharomyces cerevisiae. S. cerevisiae carrying pδCGT integrated into chromosome exhibited starch-hydrolyzing at same optimal pH and temperature as free enzyme. Volumes bread loaves...
Idiopathic pulmonary fibrosis (IPF) is a fatal lung disease with poor prognosis and progression to related genetic factors as well environmental factors. In fact, it was discovered that in South Korea many people who used humidifier disinfectants containing polyhexamethylene guanidine (PHMG), died of fibrosis. Currently two anti-fibrotic drugs, pirfenidone nintedanib, have been approved by the FDA, but unfortunately, do not cure disease. Since histone deacetylase (HDAC) activity associated...
SRC-family kinases (SFKs) have been implicated in Alzheimer's disease (AD), but their mode of action was scarcely understood. Here, we show that LYN plays an essential role amyloid β (Aβ)-triggered neurotoxicity and tau hyperphosphorylation by phosphorylating Fcγ receptor IIb2 (FcγRIIb2). We found enzyme activity increased the brain AD patients promoted neuronal cells exposed to Aβ 1–42 (Aβ1–42). Knockdown expression inhibited Aβ1–42-induced cell death. Of note, interacted with FcγRIIb2 upon...
Histone deacetylases have been a target of therapy for organ fibrosis. Here, we report the protective effect CG200745 (CG), novel histone deacetylase inhibitor, on tubulointerstitial fibrosis in Col4a3−/− mice, murine model Alport syndrome. Morphological analyses revealed CG treatment markedly alleviated kidney mice at age 7 weeks. prevented activation transforming growth factor β (TGFβ) and its downstream SMAD signaling mice. As critical upstream regulators TGFβ signaling, immunoblotting...