A5054411611- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- Congenital Diaphragmatic Hernia Studies
- Drug Transport and Resistance Mechanisms
- Tracheal and airway disorders
- Pediatric Hepatobiliary Diseases and Treatments
- Hepatitis C virus research
- Identification and Quantification in Food
- Public Administration and Governance
- Neuropeptides and Animal Physiology
- Vascular Anomalies and Treatments
- Acupuncture Treatment Research Studies
- Autoimmune and Inflammatory Disorders
- Plant-based Medicinal Research
- Autoimmune Bullous Skin Diseases
- Liver Disease and Transplantation
- Receptor Mechanisms and Signaling
- HIV/AIDS drug development and treatment
- Public Administration, ICT, and Policy Development
- Ichthyology and Marine Biology
- Infectious Diseases and Tuberculosis
- Signaling Pathways in Disease
- Streptococcal Infections and Treatments
- Phytochemical compounds biological activities
- Cardiac electrophysiology and arrhythmias
ETH Zurich
2022-2024
Beijing Chao-Yang Hospital
2024
Capital Medical University
2009-2024
Fudan University
2024
Zhongshan Hospital
2024
Zhuhai People's Hospital
2024
Jinan University
2024
Institute of Molecular Biology and Biophysics
2024
Beijing Anzhen Hospital
2024
Beijing Children’s Hospital
2020-2023
The crystal structure of the β2-adrenergic receptor (β2AR) bound to G protein adenylyl cyclase stimulatory (Gs) captured complex in a nucleotide-free state (β2AR-Gsempty). Unfortunately, β2AR-Gsempty does not provide clear explanation for coupling specificity. Evidence from several sources suggests existence transient between β2AR and GDP-bound Gs (β2AR-GsGDP) that may represent an intermediate on way formation contribute Here we present with carboxyl terminal 14 amino acids Gαs along...
Receptor-interacting protein (RIP) has been implicated in the induction of death receptor-mediated, nonapoptotic cell death. However, mechanisms remain to be elucidated. Here we show that tumor necrosis factor alpha induced RIP-dependent inhibition adenine nucleotide translocase (ANT)-conducted transport ADP into mitochondria, which resulted reduced ATP and necrotic The ADP/ATP exchange coincided with loss interaction between ANT cyclophilin D inability adopt cytosolic conformational state,...
Abstract Cellular entry of the hepatitis B and D viruses (HBV/HDV) requires binding viral surface polypeptide preS1 to hepatobiliary transporter Na + -taurocholate co-transporting (NTCP). This interaction can be blocked by bulevirtide (BLV, formerly Myrcludex B), a derivative approved drug for treating HDV infection. Here, elucidate basis this inhibitory function, we determined cryo-EM structure BLV-bound human NTCP. BLV forms two domains, plug lodged in bile salt transport tunnel NTCP...
Significance The development of selective antagonists for muscarinic acetylcholine receptors is challenging due to high homology in orthosteric binding sites among subtypes. Starting from a single amino acid difference the pockets M2 receptor (M2R) and M3R, we developed an M3R-selective antagonist using molecular docking structure-based design. resulting M3R showed up 100-fold selectivity over M2R affinity 1,000-fold vivo. docking-predicted geometry was further confirmed by 3.1 Å crystal...
BSEP (ABCB11) is an ATP-binding cassette transporter that expressed in hepatocytes and extrudes bile salts into the canaliculi of liver. dysfunction, caused by mutations or induced drugs, frequently associated with severe cholestatic liver disease. We report cryo-EM structure glibenclamide-bound human nanodiscs, revealing basis small-molecule inhibition. Glibenclamide binds apex a central binding pocket between transmembrane domains, preventing from undergoing conformational changes, thus...
Clostridium perfringens (C. perfringens) is an important foodborne pathogen that can cause diseases such as gas gangrene and necrotizing enteritis in a variety of economic animals, seriously affecting public health the benefits healthy development livestock poultry breeding industry. Perfringolysin O (PFO) virulence factor C. plays critical roles necrotic gangrene, rendering it ideal target for developing new drugs against infections caused by this pathogen. In study, based on biological...
Objective: To investigate the predictive factors for bronchitis obliterans in refractory Mycoplasma pneumoniae pneumonia (RMPP). Methods: A restrospective case summary was conducted 230 patients with RMPP admitted to Department of No.2 Respiratory Medicine Beijing Children's Hospital, Capital Medical University from January 2013 June 2017 were recruited. Clinical data, laboratory results, imaging results and follow-up data collected. Based on bronchoscopy findings 1 year after discharge, all...
Objective:To investigate the audiological characteristics of large vestibular aqueduct syndrome(LVAS) in infants and young children, to provide suggestion for early diagnosis intervention.Method:One hundred twenty-four cases diagnosed as LVAS were enrolled our study. Acoustic immittance, pediatric audiometry and(or) auditory steady state responses brainstem response test tested analyze degree configuration hearing loss, air-bone threshold difference short latency negative response.Result:The...
Objective: To provide theoretical basis for early diagnosis and accurate bronchoscopic classification of tracheobronchial tuberculosis (TBTB) in children through analyzing the clinical characteristics, classifications treatment effect with TBTB. Methods: In this respective study, we collected data patients TBTB who accepted bronchoscopies Interventional Pulmonology Department Beijing Children's Hospital Affiliated to Capital Medical University between January, 2006 December, 2019. The basic...
Cellular entry of the hepatitis B and D viruses (HBV/HDV) require binding viral surface polypeptide preS1 to hepatobiliary transporter NTCP. This interaction can be blocked by bulevirtide (BLV, formerly Myrcludex B), a derivative approved drug for treating HDV infection. To elucidate basis this inhibitory function, we determined cryo-EM structure BLV-bound human BLV forms two domains, plug lodged in bile salt transport tunnel NTCP string that covers receptors extracellular surface. The...
To investigate the clinical characteristics and efficacy of thrombus aspiration in patients with early intrastent thrombosis (EST) following carotid artery stenting (CAS).
To evaluate the clinical outcomes of thoracic endovascular aortic repair (TEVAR) in treatment Stanford type B dissection (TBAD) Marfan syndrome patients who had no history arch replacement.
ABSTRACT BACKGROUND Shenfu injection, as a traditional Chinese medicine, can alleviate reperfusion injury after ST-segment elevation myocardial infarction (STEMI) through multiple pharmacologic effects. This trial aimed to evaluate the effect of injection on in STEMI patients undergoing primary percutaneous coronary intervention (PCI). METHODS was multicenter, randomized, double-blind, parallel-group, placebo-controlled trial. First-time anterior PCI within 12 hours symptom onset due...
Objective: To explore the early clinical clues for diagnosis of chronic granulomatous disease (CGD). Methods: One hundred and thirty-nine children with CGD seen in Beijing Children's Hospital from January 2007 to October 2020 were included this study. The features including age onset, first presentations, reason being hospitalized, etiology, imaging features, all patients evaluated retrospectively. According time diagnosis, divided into two groups, cases diagnosed before 2015 after 2015....
Objective: The aim of this study was to summarize the clinical and imaging characteristics post-primary tuberculosis in children, so as improve early identification diagnosis tuberculosis. Methods: This a retrospective which enrolled children who were admitted Department No.2 Respiratory Medicine, Beijing Children's Hospital Affiliated Capital Medical University between January 2015 December 2020 with Results: A total 30 patients enrolled, including 10 males 20 females. age on admission 13.0...