A5063564241- Traumatic Brain Injury and Neurovascular Disturbances
- Intracranial Aneurysms: Treatment and Complications
- Cardiac Arrest and Resuscitation
- Neurosurgical Procedures and Complications
- Cerebrovascular and Carotid Artery Diseases
- Respiratory Support and Mechanisms
- Acute Ischemic Stroke Management
- Vascular Malformations Diagnosis and Treatment
- Neuropeptides and Animal Physiology
- Intracerebral and Subarachnoid Hemorrhage Research
- High Altitude and Hypoxia
- Thermal Regulation in Medicine
- Neurological Disorders and Treatments
- Advanced Glycation End Products research
- Intensive Care Unit Cognitive Disorders
- Non-Invasive Vital Sign Monitoring
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Heme Oxygenase-1 and Carbon Monoxide
- Neurological Disease Mechanisms and Treatments
- Mitochondrial Function and Pathology
- Epilepsy research and treatment
- Central Venous Catheters and Hemodialysis
Northwell Health
2019-2024
Feinstein Institute for Medical Research
2019-2024
Hiroshima City Asa Citizens Hospital
2016-2024
Neurological Surgery
2012-2023
Okayama University
2012-2023
Fukuyama City Hospital
2020-2021
North Shore University Hospital
2020
Chiba Institute of Science
2014
National Center for Global Health and Medicine
2014
Monash Institute of Medical Research
2005-2006
Abstract Objective: High mobility group box‐1 (HMGB1) plays an important role in triggering inflammatory responses many types of diseases. In this study, we examined the involvement HMGB1 traumatic brain injury (TBI) and evaluated ability intravenously administered neutralizing anti‐HMGB1 monoclonal antibody (mAb) to attenuate injury. Methods: Traumatic was induced rats or mice by fluid percussion. Anti‐HMGB1 mAb control after TBI. Results: remarkably inhibited percussion‐induced edema rats,...
In several biological systems, the inhibin β A homodimer activin is stimulated by, and in turn, inhibits action of interleukin (IL)-1 (both IL-1α IL-1β) IL-6. The possibility that a similar regulatory relationship operates within testis was investigated. Sertoli cells from immature (20-day-old) rats were cultured with human or IL-1β, IL-6 and/or ovine FSH dibutyryl cAMP. Activin dimers, B, measured by specific ELISA. Immunoreactive (ir-inhibin) RIA. Activin/inhibin subunit mRNA expression...
Background Cardiac arrest (CA) can induce oxidative stress after resuscitation, which causes cellular and organ damage. We hypothesized that post-resuscitation normoxic therapy would protect organs against improve oxygen metabolism survival. tested the oxygen-sensitive reactive species from mitochondria to determine association with hyperoxia-induced stress. Methods Results Sprague-Dawley rats were subjected 10-minute asphyxia-induced CA a fraction of inspired O2 0.3 or 1.0 (normoxia versus...
We measured endogenous overflow of acetylcholine (ACh) from a vascularly perfused rat stomach in vitro with modified Krebs-Ringer solution containing 100 microM physostigmine. Evoked ACh by vagal stimulation at 2.5 Hz for 2 min was abolished tetrodotoxin (3 x 10(-7) M) or Ca++ removal and reduced hexamethonium (10(-4) M). The evoked inhibited oxotremorine (10(-7) to 10(-5) M), but not attenuated 4-(N-[3-chlorophenyl]carbamoyloxy)-2-butynyltrimethylammonium chloride (10(-6) enhanced atropine...
The nature of the interaction cloned alpha 2a-adrenergic receptors from LLC-PK1-O clone cells with G proteins was investigated using an immunoprecipitation approach. Following solubilization 2a receptors, antiserum 8730, which is directed against C-terminal region Gi alpha, immunoprecipitated receptor-Gi complexes. specific since it could be blocked by peptide to 8730 generated. Antisera 3646 (anti-Gi 1), 1521 2), and 1518 3) solubilized complexes, indicating that all three subtypes couple...
The regulation of Sertoli cell activin A and inhibin B secretion during inflammation was investigated in vitro. Adult rat cells were incubated with the inflammatory mediators, lipopolysaccharide (LPS), interleukin-1β (IL-1β), IL-6 IL-1 receptor antagonist (IL-1ra) over 48 h culture. Activin A, IL-1α measured culture medium by specific two-site ELISAs. Both IL-1β- LPS-stimulated inhibited secretion. LPS also stimulated production cultures. In contrast to IL-1β, had no effect on although it...
Production and regulation of activin A inhibin B during the cycle seminiferous epithelium were investigated in adult rats. Immunohistochemistry localised β -subunit to Sertoli cell cytoplasm, with much weaker expression spermatocytes spermatids. Both B, measured by ELISA secreted by, tubule fragments over 72 h culture. Activin was a cyclic manner peak secretion from tubules isolated at stage VIII. Tubules collected VI produced least A. Inhibin highest IX-I lowest VII tubules. Addition...
Abstract Cardiac arrest (CA) patients suffer from systemic ischemia–reperfusion (IR) injury leading to multiple organ failure; however, few studies have focused on tissue-specific pathophysiological responses IR-induced oxidative stress. Herein, we investigated biological and physiological parameters of the brain heart, particularly lung dysfunction that has not been well studied date. We aimed understand susceptibility stress tested how oxygen concentrations in post-resuscitation setting...
We developed an experimental in vitro model to detect a very small amount of endogenous noradrenaline (NA) released from the rat gastric sympathetic nerve terminals. The stomach was perfused via celiac artery with modified Krebs-Ringer solution containing 10 mM pargyline and 0.1% bovine serum albumin at constant flow 4 ml/min. right greater splanchnic (SPL) (preganglionic nerve) stimulated electrically square-wave pulses 2 msec duration supramaximal intensity (5 mA) for 1 min. contained...
Abstract Background High-quality cardiopulmonary resuscitation (HQ-CPR) is of paramount importance to improve neurological outcomes cardiac arrest (CA). The purpose this study was evaluate chest compression methods by combining two theories: and thoracic pumps. Methods Male Sprague-Dawley rats were used. Three types studied. 1-side method performed vertically with 2 fingers over the sternum. 2-side horizontally fingers, bilaterally squeezing wall. 3-side combined methods. Rats underwent 10...
Abstract Using a new method for measuring the molecular ratio (R) of inhalation to exhalation, we investigated effect high fraction inspired oxygen (FIO2) on consumption (VO2), carbon dioxide generation (VCO2), and respiratory quotient (RQ) in mechanically ventilated rats. Twelve rats were equally assigned into two groups by anesthetics: intravenous midazolam/fentanyl vs. inhaled isoflurane. R, VO2, VCO2, RQ measured at FIO2 0.3 or 1.0. R error was ± 0.003. 1.0099 0.0023 with isoflurane...
OBJECTIVES: Cardiac arrest and subsequent resuscitation have been shown to deplete plasma phospholipids. This depletion of phospholipids in circulating may contribute organ damage postresuscitation. Our aim was identify the diminishment essential postresuscitation develop a novel therapeutic approach supplementing these depleted that are required prevent dysfunction postcardiac arrest, which lead improved survival. DESIGN: Clinical case control study followed by translational laboratory...
Mechanisms related to the gastric antisecretory action of i.c.v.-administered prostaglandins (PGs) were investigated in urethane-anesthetized rats with fistula. The acid output was enhanced by electrical stimulation left cervical vagus nerve after cutting bilateral nerves. Intracerebroventricular administered PGE2 (0.05-0.5 microgram/animal) dose-dependently inhibited vagally stimulated whereas same doses i.v. without effect. inhibitory effect (0.1 microgram/animal, i.c.v.) more potent than...