A5064725145- Advanced Glycation End Products research
- Gear and Bearing Dynamics Analysis
- Mechanical Engineering and Vibrations Research
- Immune Response and Inflammation
- Metallurgy and Material Forming
- Engineering Applied Research
- Monoclonal and Polyclonal Antibodies Research
- Mechanical stress and fatigue analysis
- Immune Cell Function and Interaction
- Advanced Fiber Laser Technologies
- Advanced ceramic materials synthesis
- Mast cells and histamine
- Laser-Matter Interactions and Applications
- S100 Proteins and Annexins
- Nicotinic Acetylcholine Receptors Study
- Inflammasome and immune disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Injection Molding Process and Properties
- Protein Structure and Dynamics
- Cell Adhesion Molecules Research
- Glycosylation and Glycoproteins Research
- Metal Alloys Wear and Properties
- Inflammatory mediators and NSAID effects
- Ion channel regulation and function
- Natural Antidiabetic Agents Studies
Kindai University
2016-2025
Shujitsu University
2009-2023
Okayama University
2004-2023
National Institute of Technology, Kisarazu College
2012-2022
Musashino Red Cross Hospital
2021
Tokyo Medical and Dental University
2008-2021
Yokohama City University
2010-2020
Japanese Red Cross Society, Japan
1989-2020
National Institute of Advanced Industrial Science and Technology
2007-2018
The University of Tokyo
1995-2016
Because cytotoxic T lymphocytes (CTL) may be important for preventing direct cell-to-cell transmission of human immunodeficiency virus (HIV), the agent responsible acquired syndrome, we have begun to investigate epitope specificity and immune response (Ir) gene control anti-HIV CTL responses in experimental animals. Mice were infected with a recombinant vaccinia expressing HIV gp160 envelope gene, primed restimulated vitro transfected histocompatible cell line same gene. Our results show...
The high mobility group box-1 (HMGB1), originally identified as an architectural nuclear protein, exhibits inflammatory cytokine-like activity in the extracellular space. Here we show that treatment with neutralizing anti-HMGB1 monoclonal antibody (mAb; 200 microg, twice) remarkably ameliorated brain infarction induced by 2-h occlusion of middle cerebral artery rats, even when mAb was administered after start reperfusion. Consistent 90% reduction infarct size, accompanying neurological...
Background and Purpose— High mobility group box-1 (HMGB1) exhibits inflammatory cytokine-like activity in the extracellular space. We previously demonstrated that intravenous injection of anti-HMGB1 monoclonal antibody (mAb) remarkably ameliorated brain infarction induced by middle cerebral artery occlusion rats. In present study, we focused on protective effects mAb marked translocation HMGB1 brain, disruption blood–brain barrier (BBB), resultant edema. Methods— Middle rat was used as...
Abstract Objective: High mobility group box‐1 (HMGB1) plays an important role in triggering inflammatory responses many types of diseases. In this study, we examined the involvement HMGB1 traumatic brain injury (TBI) and evaluated ability intravenously administered neutralizing anti‐HMGB1 monoclonal antibody (mAb) to attenuate injury. Methods: Traumatic was induced rats or mice by fluid percussion. Anti‐HMGB1 mAb control after TBI. Results: remarkably inhibited percussion‐induced edema rats,...
We demonstrated single-shot measurements of spectral interference between a white-light continuum generated in hollow-fiber and its second harmonic. The has information on the carrier-envelope phase an input pulse to fiber time delay blue wing continuum. By analyzing observed interference, we estimated shot-by-shot changes phase. This method is useful for determining low-repetition-rate, high-intensity laser.
DNA complementary to the bovine retinal mRNA coding for β‐subunit of transducin has been cloned by screening a cDNA library with oligodeoxyribonucleotide probes. Nucleotide sequence analysis revealed that this polypeptide consists 340 amino acid residues (including initiating methionine). Furthermore, hybridizable probe from derived brain poly(A) + RNA. Comparison cDNAs, in conjunction blot hybridization and S 1 nuclease mapping RNA retina, liver, suggests mRNAs β‐subunits other guanine...
The purpose of this investigation was to examine the effect filler particle size and shape as well content on light transmittance characteristics color experimental composite resins. A mixture 30 mol% Bis-GMA 70 TEGDMA prepared a base monomer which photoinitiator (camphorquinone) co-initiator (N,N-dimethylaminoethyl methacrylate) were added. Four different irregular- spherical-shaped types with an average 1.9-11.1 μm added in three contents 20, 30, 40 vol%. Light including diffusion...
The chemokine stromal cell-derived factor-1 (SDF-1/CXCL12) and its G-protein-coupled receptor (GPCR) CXCR4 play fundamental roles in many physiological processes, is a drug target for various diseases such as cancer metastasis human immunodeficiency virus, type 1, infection. However, almost no structural information about the SDF-1-CXCR4 interaction available, mainly because of difficulties expression, purification, crystallization CXCR4. In this study, an extensive investigation preparation...
High-mobility group box protein 1 (HMGB1) is a DNA-binding and cytokine highly expressed in atherosclerotic lesions, but its pathophysiological role atherosclerosis unknown. We investigated the development of ApoE-/- mice.Apolipoprotein E-deficient (ApoE-/-) mice fed high-fat diet were administered monoclonal anti-HMGB1 neutralizing antibody, effects on lesion size, immune cell accumulation, proinflammatory mediators assessed using Oil Red O, immunohistochemistry, real-time polymerase chain...
Inflammatory processes in brain tissue have been described human epilepsy of various etiologies and experimental models seizures. High mobility group box-1 (HMGB1) is now recognized as representative damage-associated molecular patterns (DAMPs). In the present study, we focused on whether anti-HMGB1 antibody treatment could relieve status epilepticus- triggered BBB breakdown inflammation response addition to seizure behavior itself. Pilocarpine methyl-scopolamine were used establish acute...
The transcription factor NtcA is a global regulator of nitrogen homeostasis in cyanobacteria. It thus positively regulates the expression genes related to assimilation such as glnA (which encodes glutamine synthetase) and ntcA itself response shortage or depletion. binding promoters Synechococcus sp. PCC 7942 vitro now has been shown be enhanced by 2-oxoglutarate. In analysis gene also revealed that interaction with its promoter element was not sufficient for activation transcription,...
The purpose of this study was to investigate effects various visible light photoinitiators on the polymerization efficiency and color light-activated resins. Four photoinitiators, including camphorquinone, phenylpropanedione, monoacrylphosphine oxide (TPO), bisacrylphosphine (Ir819), were used. Each photoinitiator dissolved in a Bis-GMA TEGDMA monomer mixture. Materials polymerized using dental quartz-tungsten halogen lamp (QTH), plasma-ark blue LED light-curing units, custom-made violet...
Gastric inhibitory polypeptide (GIP) is a 42-amino acid hormone that stimulates insulin secretion in the presence of glucose. Complementary DNA clones encoding human GIP were isolated from library prepared with RNA duodenum. The predicted amino sequence indicates derived by proteolytic processing 153-residue precursor, preproGIP. moiety flanked segments 51 and 60 acids at its NH2 COOH termini, respectively. former includes signal peptide about 21 residues an NH2-terminal propeptide 30 acids....
Sepsis is a major cause of death worldwide. We show that plasma protein histidine-rich glycoprotein (HRG) was decreased significantly in septic mice with cecal ligation and puncture (CLP) supplementary treatment exogenous HRG improved survival, strong inhibition tight attachment neutrophils to pulmonary vasculatures, subsequent immunothrombosis, DIC state, lung inflammation, hypercytokinemia, activation vascular endothelial cells (VECs). In contrast, knockdown by siRNA exacerbated lethality....