A5072080063- Spectroscopy and Quantum Chemical Studies
- Molecular spectroscopy and chirality
- Advanced Chemical Physics Studies
- Lipid Membrane Structure and Behavior
- Photosynthetic Processes and Mechanisms
- Immune Cell Function and Interaction
- Spectroscopy and Laser Applications
- Systemic Lupus Erythematosus Research
- Plant-Microbe Interactions and Immunity
- Mass Spectrometry Techniques and Applications
- Advanced Glycation End Products research
- Atmospheric chemistry and aerosols
- Reproductive System and Pregnancy
- Occupational Health and Performance
- Polysaccharides and Plant Cell Walls
- T-cell and B-cell Immunology
- Photoreceptor and optogenetics research
- Monoclonal and Polyclonal Antibodies Research
- Petroleum Processing and Analysis
- Molecular Spectroscopy and Structure
- Hydrocarbon exploration and reservoir analysis
- Enhanced Oil Recovery Techniques
- Atmospheric Ozone and Climate
- Chemical Thermodynamics and Molecular Structure
- Cytomegalovirus and herpesvirus research
Yokohama City University
1995-2025
Yokohama City University Hospital
2025
Sanofi (United States)
2019-2022
Pacific Northwest National Laboratory
2014-2020
Environmental Molecular Sciences Laboratory
2014-2020
John Wiley & Sons (United States)
2014-2020
Yale University
2009-2020
Okayama University
2017-2020
Battelle
2015-2019
University of Alberta
2011-2019
In situ and real-time characterization of protein secondary structures at interfaces is important in biological bioengineering sciences, yet remains technically challenging. this study, we used chiral sum frequency generation (SFG) spectroscopy to establish a set vibrational optical markers for characterizing interfaces. We discovered that the N-H stretches along peptide backbones α-helices can be detected SFG spectra. further observed signatures stretch together with amide I are unique...
Sum-frequency generation vibrational spectroscopy (SFG-VS) can provide detailed information and understanding of the molecular composition, interactions, orientational conformational structure surfaces interfaces through quantitative measurement analysis. In this review, we present current status discuss important recent developments in intrinsic SFG spectral lineshapes formulations for polarization measurements analysis SFG-VS spectra. The focus review is to a coherent description main...
Kinetic analysis of conformational changes proteins at interfaces is crucial for understanding many biological processes membrane surfaces. In this study, we demonstrate that surface-selective sum frequency generation (SFG) spectroscopy can be used to investigate kinetics interfaces. We focus on an intrinsically disordered protein, human islet amyloid polypeptide (hIAPP) known misfold into the β-sheet structure upon interaction with membranes. Using ssp polarization setting (s-polarized SFG,...
Colloidal interactions in dilute solution singularly enable selection of well-behaved antibodies during drug discovery.
Inflammatory processes in brain tissue have been described human epilepsy of various etiologies and experimental models seizures. High mobility group box-1 (HMGB1) is now recognized as representative damage-associated molecular patterns (DAMPs). In the present study, we focused on whether anti-HMGB1 antibody treatment could relieve status epilepticus- triggered BBB breakdown inflammation response addition to seizure behavior itself. Pilocarpine methyl-scopolamine were used establish acute...
This work presents molecular-level investigations of how well-characterized silica-supported phospholipid bilayers formed from either pure DOPC or a 9:1 mixture DOPC:DOTAP interact with positively and negatively charged 4 nm gold metal nanoparticles at pH 7.4 NaCl concentrations ranging 0.001 to 0.1 M. Second harmonic generation (SHG) charge screening measurements indicate the supported carry negative interfacial potential. Resonantly enhanced SHG probing electronic transitions within core...
Studying hydrogen/deuterium (H/D) exchange in proteins can provide valuable insight on protein structure and dynamics. Several techniques are available for probing H/D the bulk solution, including NMR, mass spectroscopy, Fourier transform infrared spectroscopy. However, at interfaces is challenging because it requires surface-selective methods. Here, we introduce combination of situ chiral sum frequency generation (cSFG) spectroscopy ab initio simulations cSFG spectra as a powerful...
Characterizations of protein structures at interfaces are important in solving an array fundamental and engineering problems, including understanding transmembrane signal transduction molecular transport processes development biomaterials to meet the needs biomedical energy research. However, situ real-time characterization secondary is challenging because it requires physical methods that selective both interface structures. Here, we summarize recent experimental developments our laboratory...
Reliable determination of the spectral features and their phases in sum-frequency generation vibrational spectroscopy (SFG-VS) for surfaces with closely overlapping peaks has been a standing issue. Here we present two approaches toward resolving such The first utilizes high-resolution accurate line shape from recently developed subwavenumber broadband SFG-VS (HR-BB-SFG-VS), which detail parameters, including relative phases, can be determined through reliable fitting. These results are...
Abstract Background Effector proteins function not only as toxins to induce plant cell death, but also enable pathogens suppress or evade defense responses. NLP-like are considered be effector proteins, and they have been isolated from bacteria, fungi, oomycete pathogens. There is increasing evidence that NLPs the ability death ethylene accumulation in plants. Results We evaluated expression patterns of 11 targeted PcNLP genes by qRT-PCR at different time points after infection P. capsici ....
Abstract Here we show that the addition of chemical interactions between soluble monosaccharides and an insoluble lipid surfactant monolayer improves agreement modeled sea spray chemistry with observed marine aerosol chemistry. In particular, alkane:hydroxyl mass ratio in organic matter is reduced from a median 2.73 to range 0.41–0.69, reducing discrepancy previous Fourier transform infrared spectroscopy (FTIR) observations clean (ratio: 0.24–0.38). The overall fraction submicron also...
In the present study, we have investigated growth of a multilayer formed by poly(sodium 4-styrene sulfonate) (PSSS) and poly(diallyldimethylammonium chloride) (PDDA) at different salt concentrations use quartz crystal microbalance with dissipation (QCM-D). The frequency change (Deltaf) demonstrates that exponential mode gradually becomes dominant as NaCl concentration (C(NaCl)) increases. On other hand, (DeltaD) reveals deposition is dominated chain conformation C(NaCl) < 1.0 M, where...
Cell membranes are crucial to many biological processes. Because of their complexity, however, lipid bilayers often used as model systems. Lipid structures influence the physical properties bilayers, but interplay, especially in multiple-component has not been fully explored. Here, we Langmuir-Blodgett method make mono- and 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (DPPG), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (POPG),...
Abstract NK cell functions are tightly regulated by the balance between inhibitory and stimulatory surface receptors. We investigated expression of galectin-9 (Gal-9) its function in cells from HIV-infected individuals on antiretroviral therapy, long-term nonprogressors, progressors compared with healthy controls. also measured TIGIT TIM-3 different subpopulations their functionality to Gal-9+ cells. Our data demonstrated significant upregulation Gal-9 groups versus was associated impaired...
We have built a broad bandwidth vibrational sum frequency generation (VSFG) spectrometer that can provide high-quality spectra over the range of 3800 to 900 cm −1 . The contains commercial Ti:sapphire based 6 W regenerative amplifier as master light source, home-built pulse shaper produce narrow 800 nm beam, optical parametric generate femtosecond infrared (IR) pulse, and detection system with monochromator charge-coupled device (CCD). applied this obtain VSFG lipid monolayer at air–water...
Self-assembly of molecules into chiral macromolecular and supramolecular structures at interfaces is important in various fields, such as biomedicine, polymer sciences, material chemistry. However, probing the kinetics remains challenging because it requires a real-time method that has selectivity to both interface chirality. Here, we introduce an situ approach using C-H stretch vibrational probe detected by sum frequency generation spectroscopy (cSFG). We showed cSFG signals amphiphilic...
Characterization of protein secondary structures at interfaces is still challenging due to the limitations surface-selective optical techniques. Here, we address challenge characterizing parallel β-sheets by combining chiral sum frequency generation (SFG) spectroscopy and computational modeling. We focus on human islet amyloid polypeptide aggregates a de novo designed short lipid/water air/glass interfaces. find that adopt distinct orientations various exhibit characteristic chiroptical...
We report in this work detailed measurements of the chiral and achiral sum-frequency vibrational spectra C-H stretching vibration region (2800-3050 cm(-1)) air/liquid interfaces R-(+)-limonene S-(-)-limonene, using recently developed high-resolution broadband generation spectroscopy (HR-BB-SFG-VS). The SFG R-limonene S-limonene, as well RS racemic mixture (50/50 equal amount mixture), show that corresponding molecular groups R S enantiomers are with same interfacial orientations....
Biomolecular hydration is fundamental to biological functions. Using phase-resolved chiral sum-frequency generation spectroscopy (SFG), we probe molecular architectures and interactions of water molecules around a self-assembling antiparallel β-sheet protein. We find that the phase chiroptical response from O-H stretching vibrational modes flips with absolute chirality (l-) or (d-) β-sheet. Therefore, can conclude organizes solvent into supermolecular structure opposite handedness relative...