A5034502901- Thyroid Disorders and Treatments
- Malaria Research and Control
- Neonatal Health and Biochemistry
- Invertebrate Immune Response Mechanisms
- Mosquito-borne diseases and control
- Insect Resistance and Genetics
- HIV/AIDS drug development and treatment
- Growth Hormone and Insulin-like Growth Factors
- Epigenetics and DNA Methylation
- Neuroscience of respiration and sleep
- Cancer-related gene regulation
- HIV Research and Treatment
- Pregnancy and preeclampsia studies
- RNA modifications and cancer
- Histone Deacetylase Inhibitors Research
- Synthesis and Catalytic Reactions
- vaccines and immunoinformatics approaches
- Natural product bioactivities and synthesis
- Melanoma and MAPK Pathways
- Mitochondrial Function and Pathology
- Ion Transport and Channel Regulation
- Wnt/β-catenin signaling in development and cancer
- Research on Leishmaniasis Studies
- Essential Oils and Antimicrobial Activity
- Phytochemicals and Medicinal Plants
China Medical University
2013-2024
University of South Florida
2019-2024
Institute of Oceanology
2024
Laoshan Laboratory
2024
The Fourth People's Hospital
2019
Wuhan No. 7 Hospital
2016
Rutgers, The State University of New Jersey
1994
Artemisinin resistance has emerged in Southeast Asia, endangering the substantial progress malaria elimination worldwide. It is associated with mutations PfK13 protein, but how mediates artemisinin not completely understood. Here we used a new antibody against to show that protein expressed all stages of asexual intraerythrocytic cycle as well gametocytes and partially localized endoplasmic reticulum. By introducing four into 3D7 strain reverting these field parasite isolates, determined...
Abstract Although artemisinin combination therapies have succeeded in reducing the global burden of malaria, multidrug resistance deadliest malaria parasite, Plasmodium falciparum , is emerging worldwide. Innovative antimalarial drugs that kill all life-cycle stages parasites are urgently needed. Here, we report discovery compound JX21108 with broad antiplasmodial activity against multiple parasites. was developed from chemical optimization quisinostat, a histone deacetylase inhibitor. We...
The histone acetyltransferase GCN5-associated SAGA complex is evolutionarily conserved from yeast to human and functions as a general transcription co-activator in global gene regulation. In this study, we identified divergent GCN5 Plasmodium falciparum , which contains two plant homeodomain (PHD) proteins (PfPHD1 PfPHD2) apetela2 (AP2)-domain factor (PfAP2-LT). To dissect the of PfGCN5 complex, generated parasite lines with either bromodomain or PHD domain PfPHD1 deleted. deletion mutants...
Our previous work found that the clinical histone deacetylase (HDAC) inhibitor quisinostat exhibited a significant antimalarial effect but with severe toxicity. In this work, 35 novel derivatives were designed and synthesized based on as lead compound, their in vitro activities cytotoxicities systematically evaluated. Among them, JX35 showed potent inhibition against both wild-type multidrug-resistant parasite strains displayed vivo killing all life cycles of parasites, including blood...
Abstract Among the GTPase family members, guanylate‐binding protein‐1 (GBP‐1) is most thoroughly studied member in a plethora of human cancers. GBP‐2, on other hand, remains limitedly studied. We wonder how GBP‐2 participates colorectal carcinoma (CRC) as well paclitaxel (PTX)‐resistance CRC. In this study, authors are determined to dig into role that plays sensitivity CRC PTX, therefore, possibly indicating promising gene therapy target for Forced expression was done by plasmid...
Abstract In malaria parasites, the regulation of mRNA translation, storage and degradation during development life-stage transitions remains largely unknown. Here, we functionally characterized DEAD-box RNA helicase PfDOZI in P. falciparum . Disruption pfdozi enhanced asexual proliferation but reduced sexual commitment impaired gametocyte development. By quantitative transcriptomics, show that is involved invasion-related genes stage-specific different developmental stages. predominantly...
Aging is a significant risk factor for the increased incidence of acute kidney injury and chronic disease, posing challenges to global public health. The role N6-methyladenosine (m6A) in development disease has been reported, but regulatory mechanism m6A aging remains unclear. In this study, we identified long non-coding RNA (lncRNA), called TUG1, which exhibited significantly decreased level modification human aged through m6A-lncRNA epitranscriptome microarray. Bioinformatics analysis...
Iodine is essential for the biosynthesis of thyroid hormones, including triiodothyronine and thyroxine. Thyroid hormones are important central nervous system development. Mild maternal iodine deficiency ( ID )‐induced hypothyroxinaemia causes neurological deficits mental retardation foetus. However, detailed mechanism underlying these still largely unknown. Given that growth‐associated protein 43 kDa GAP ‐43), s emaphorin 3 A (Sema3 ) glycogen synthase kinase 3β GSK 3β)/collapsin response...
Iodine is essential for the synthesis of thyroid hormone. deficiency (ID)-induced hypothyroxinaemia and hypothyroidism during developmental period contribute to impairments function in brain, such as psychomotor motor alterations. However, mechanisms are still unclear. Therefore, present research study effects caused by mild ID severe or methimazole (MMZ) on dendritic growth filial cerebellar Purkinje cells (PCs) underlying mechanisms.A maternal model was established Wistar rats using a...
Protein arginine methyltransferases (PRMTs) regulate many important cellular processes, such as transcription and RNA processing in model organisms but their functions human malaria parasites are not elucidated. Here, we characterize PfPRMT5 Plasmodium falciparum, which catalyzes symmetric dimethylation of histone H3 at R2 (H3R2me2s) R8, H4 R3 vitro. disruption results asexual stage growth defects primarily due to lower invasion efficiency the merozoites. Transcriptomic analysis reveals...
Background Malaria transmission-blocking vaccines (TBVs) aim to inhibit malaria parasite development in mosquitoes and prevent further transmission the human host. The putative-secreted ookinete protein 25 (PSOP25), highly conserved Plasmodium spp., is a promising TBV target. Here, we investigated PvPSOP25 from P . vivax as candidate using transgenic murine berghei clinical isolates. Methods findings A line expressing ( TrPvPSOP25Pb ) was generated. Full-length expressed yeast Pichia...
There is an urgent need to develop new efficacious antimalarials address the emerging drug-resistant clinical cases. Our previous phenotypic screening identified styrylquinoline UCF501 as a promising antimalarial compound. To optimize UCF501, we herein report detailed structure–activity relationship study of 2-arylvinylquinolines, leading discovery potent, low nanomolar antiplasmodial compounds against Plasmodium falciparum CQ-resistant Dd2 strain, with excellent selectivity profiles...
By fusing catalytically dead Cas9 (dCas9) to active domains of histone deacetylase (Sir2a) or acetyltransferase (GCN5), this CRISPR interference/activation (CRISPRi/a) system allows gene regulation at the transcriptional level without causing permanent changes in parasite genome. However, constitutive expression dCas9 poses a challenge for studying essential genes, which may lead adaptive parasite, masking true phenotypes. Here, we developed leak-free inducible CRISPRi/a by integrating...
Sexual reproduction is an essential process in the Plasmodium life cycle and a vulnerable step for blocking transmission from human host to mosquitoes. In this study, we characterized functions of conserved cell membrane protein P115 rodent malaria parasite berghei ANKA. Pb115 was expressed both asexual stages (schizonts) sexual (gametocytes, gametes ookinetes), localized on plasma ookinetes. P. berghei, genetic deletion (Δpb115) did not affect multiplication, nor it gametocyte development...
We investigated the effects of neferine (Nef) on STI571 sensitivity and possible mechanism in STI571-resistant K562/G01 cells. observed cell proliferation by modified MTT (methyl thiazolyl tetrazolium) assay. determined intracellular concentration cells high-performance liquid chromatography (HPLC), expression P-glycoprotein (P-gp) Western blotting, MDR-1 mRNA reverse transcriptase-polymerase chain reaction (RT-PCR). that drug resistance to for was 43.99-fold higher than K562 also a low Nef...
Gametogenesis, the formation of gametes from gametocytes, an essential step for malaria parasite transmission, is targeted by transmission-blocking drugs and vaccines. We identified a conserved protein (PBANKA_0305900) in Plasmodium berghei, which encodes 22 kDa (thus named Pb22) expressed both asexual stages gametocytes. Its homologues are present all species its closely related, Hepatocystis, but not other apicomplexans. Pb22 was localised cytosols schizonts, as well male female During...
ABSTRACT Hypothyroxinemia induced by maternal mild iodine deficiency causes neurological deficits and impairments of brain function in offspring. is prevalent developing developed countries alike. However, the mechanism underlying these remains less well known. Given that myelin plays an important role learning memory function, we hypothesize hippocampal myelinated growth may be impaired rat offspring exposed to hypothyroxinemia deficiency. To test this hypothesis, female Wistar rats were...