A5000122541- Nanoparticle-Based Drug Delivery
- Diamond and Carbon-based Materials Research
- Immune Cell Function and Interaction
- Graphene and Nanomaterials Applications
- RNA Interference and Gene Delivery
- Nanoparticles: synthesis and applications
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Medicinal Plants and Neuroprotection
- Wound Healing and Treatments
- Advanced biosensing and bioanalysis techniques
- Electrospun Nanofibers in Biomedical Applications
- Extracellular vesicles in disease
- Glycosylation and Glycoproteins Research
- Neuroscience and Neural Engineering
- Immune Response and Inflammation
- Pancreatic and Hepatic Oncology Research
- Biocrusts and Microbial Ecology
- Genomic variations and chromosomal abnormalities
- Cancer Research and Treatments
- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Cancer Treatment and Pharmacology
- Dyeing and Modifying Textile Fibers
- Immune cells in cancer
Czech Academy of Sciences
2009-2024
Technical University of Liberec
2024
Czech Academy of Sciences, Institute of Microbiology
2013-2024
Czech Technical University in Prague
2016-2020
Australian University, Kuwait
2020
National Cancer Institute
2008-2009
Center for Cancer Research
2009
Efficient delivery of stabilized nucleic acids (NAs) into cells and release the NA payload are crucial points in transfection process. Here we report on fabrication a nanoscopic cellular carrier that is additionally combined with label-free intracellular sensor device, based biocompatible fluorescent nanodiamond particles. The sensing function engineered nanodiamonds by using nitrogen-vacancy color centers, providing stable non-blinking luminescence. device used for monitoring cells....
In this study, gold nanoparticles produced by eukaryotic cell waste (AuNP), were analyzed as a transfection tool. AuNP Fusarium oxysporum and spectrophotometry, transmission electron microscopy (TEM), scanning (SEM), energy dispersive X-ray spectroscopy (EDS). Fourier transform infrared (FTIR) dynamic light scattering (DLS) used before after conjugation with different nucleic acid (NA) types. Graphite furnace atomic absorption (GF-AAS) was to determine the concentration. Conjugation detected...
Background/Aim: Current research of prostate cancer (PCa) offers a promising way identifying patients with adverse prognosis who do benefit from radical treatment that can affect quality life as resections are associated numerous side-effects. The aim our study was to evaluate the relationship TMPRSS2-ERG fusion gene status, tumor tissue prostate-specific antigen (PSA), 3 (PCA3), miR-23b, miR-26a and miR-221 expression levels in combination preoperative serum PSA level risk PCa recurrence...
The mycelium-free supernatant (MFS) of a five-day-old culture medium Fusarium oxysporum was used to synthesize gold nanoparticles (AuNPs). experimental design the study answer question: can this production process AuNPs be controllable like classical chemical or physical approaches? producing from 1 mM tetrachloroauric (III) acid trihydrate in MFS monitored visually by color change at different pH values and quantified spectroscopically. produced were analyzed transmission electron...
Abstract Enzymotherapy based on DNase I or RNase A has often been suggested as an optional strategy for cancer treatment. The efficacy of such procedures is limited e.g. by a short half-time the enzymes low rate their internalization. use nanoparticles, gold nanoparticles (AuNPs), helps to overcome these limits. Specifically, biologically produced AuNPs represent interesting variant here due naturally occurring capping agents (CA) surface. composition CA depends producing microorganism. CAs...
Abstract The enormous therapeutic potential of selective ribonucleic acid (RNA) interference has recently been manifested by the approval several small interfering RNA (siRNA)‐based drugs. However, efficacy siRNA delivery is still limited, and an extensive search for alternative highly effective approaches ongoing. With this aim, three generations non‐viral vectors based on modified nanodiamonds (NDs) have gradually developed in past decade. They show great promise due to negligible toxicity...
Abstract The biologically produced gold nanoparticles (AuNPs) are novel carriers with promising use in targeted tumor therapy. Still, there no studies regarding the efficacy of nanoparticle internalization by cancer and noncancer cells. In this study, AuNPs were Fusarium oxysporum analyzed spectrophotometry, transmission electron microscopy (TEM), energy dispersive x‐ray spectroscopy (EDS), Zetasizer. Obtained about 15 nm size a zeta potential –35.8 mV. added to cells (4T1), (NIH/3T3),...
MicroRNAs are short molecules of RNA regulating most cellular processes via the mechanism interference. Their dysregulation leads to a disease burden, making them important therapeutic targets. For successful development device, uptake functionalized carrier by live cells and sufficient release effector limiting factors. Here for first time, inhibition oncogenic microRNA-21 in CT-26 colon cancer is achieved, using an advanced nanosystem consisting fluorescent nanodiamond antisense RNA....
Nanodiamonds (ND) serve as RNA carriers with potential for in vivo application. ND coatings and their administration strategy significantly change fate, toxicity, effectivity within a multicellular system. Our goal was to develop multiple coating effective delivery vivo. final complex (NDA135b) consisted of ND, polymer, antisense RNA, transferrin. We aimed (i) assess if tumor-specific promotes NDA135b tumor accumulation inhibition oncogenic microRNA-135b (ii) outline off-targets immune cell...
Aberrant glycosylation, which impairs recognition capability of NK cells or modifies pattern target cells, is associated with cancer. Synthetic glycoconjugates (GCs), modulate cell increase the sensitivity tumor to therapy boost anti-cancer immune response. In current study, we employed N-acetyl-D-glucosamine-calix[4]arene (GN4C) as a modulator glycosylation represented by NK-92 line and fresh human cells. For first time, have demonstrated that calix[4]arene-based GC down-regulated...
Although there are several research articles on the detection and characterization of protein corona surface various nanoparticles, no detailed studies formation, detection, biologically produced gold nanoparticles (AuNPs). AuNPs were prepared from Fusarium oxysporum at two different temperatures characterized by spectrophotometry, Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), energy-dispersive X-ray (EDS). The zeta potential was determined using a...
The extracellular vesicles (EVs) serve for cell-to-cell communication and delivery of signaling molecules they represent interesting approach in targeted therapy. This study focuses on an EV-mediated transfer functionalized nanoparticles into recipient tumor cells. We used biologically produced gold (AuNPs) with known physical-chemical properties decorated them inhibitory RNA targeting oncogenic microRNA-135b (AuNPs-antimiR 135 b) transferrin b-Tf). AuNPs were added to the breast cancer 4T1...
This study recognized biologically produced gold nanoparticles (AuNPs) as multiple cargo carriers with a perspective of drug delivery into specialized tumor cells in vivo. Paclitaxel (PTX), transferrin, and antimiR-135b were conjugated AuNPs their uptake by mouse an induced breast cancer model was investigated. Each the above-mentioned molecules to separately well simultaneously, loading efficiency each assessed, performance final product (FP) judged. After induction BALB/c mice, sub-IC
Non-healing wounds are a serious complication in diabetic patients. One of the detrimental factors contributing to limited wound healing is accumulation metalloproteinase-9 (MMP-9) wound. Selective inhibition MMP-9 one established therapeutic targets for healing. Here, functional and biocompatible dressing developed enable controlled release traceable vector loaded with antisense siRNA against The consists degradable polymer nanofibers embedded nanosystem - polymer-coated fluorescent...
This article examines the ability of newly developed nanostructured porous boron‐doped diamond (BDD) to form an interface with neural cells and role molecular functionalization by a polymer on this interface. Due its high stability, biocompatibility, electrical properties, BDD is promising material for construction neuroelectrodes. Nanostructuring increase in surface specific area can further improve sensitivity performance such electrodes. Here, prepared multistep deposition template...
This study recognized biologically produced gold nanoparticles (AuNP) as multiple cargo carriers with a perspective of drugs delivery into specialized tumor cells in vivo. Paclitaxel (PTX), transferrin, and antimiR-135b were conjugated AuNPs their uptake by mouse an induced breast cancer model was investigated. Each the above-mentioned molecules to separately well simultaneously, loading efficiency each assessed, performance final product (FP) assessed. After induction BALB/c mice, sub-IC50...
The determination of patient's resistance to a particular drug contributes more efficient therapeutical approach. aim this study was evaluate if the responsiveness Chronic Myeloid Leukemia (CML) patients Imatinib therapy predictable from WT1 gene expression in peripheral blood leukocytes. To examine we implemented an vitro cultivation primary cells 48 CML with Imatinib. effect characterized not only by but also BCR-ABL, and proliferative factor Ki-67. <br />Our results showed that leukocytes...
Abstract The study aims to compare different approaches and efficacies during the biological production of nanoparticles (NPs). Gold (AuNPs) are produced by Fusarium oxysporum at two temperatures. One flask is incubated 37 °C (“Common”) other directly heated for 5 min 80 (“Heat‐treated”). Obtained AuNPs analyzed compared spectrophotometry, transmission electron microscopy (TEM), scanning (SEM), energy dispersive X‐ray spectroscopy (EDS), dynamic light scattering (DLS), Fourier transform...