A5004424802- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Tryptophan and brain disorders
- Immune Response and Inflammation
- Plant and fungal interactions
- Stress Responses and Cortisol
- Diamond and Carbon-based Materials Research
- Neuroscience and Neuropharmacology Research
- Pharmacological Receptor Mechanisms and Effects
- Cancer, Stress, Anesthesia, and Immune Response
- Attachment and Relationship Dynamics
- RNA Interference and Gene Delivery
- Toxin Mechanisms and Immunotoxins
- Gut microbiota and health
- Carbon Nanotubes in Composites
- Neuropeptides and Animal Physiology
- Ion-surface interactions and analysis
- Diabetes and associated disorders
- Polyamine Metabolism and Applications
- Reproductive System and Pregnancy
- Evolutionary Psychology and Human Behavior
- Optimism, Hope, and Well-being
- Amino Acid Enzymes and Metabolism
Charles University
1994-2024
Apigenex (Czechia)
2021
National Institute of Mental Health
2021
Czech Technical University in Prague
2014-2016
Czech Academy of Sciences, Institute of Microbiology
2002-2016
Czech Academy of Sciences
1997-2014
MRC Laboratory of Molecular Biology
2014
Institute of Microbiology
2008
University Hospital Brno
2002
Masaryk University
2002
Abstract High biocompatibility, variable size ranging from ≈5 nm, stable luminescence its color centers, and simple carbon chemistry for biomolecule grafting make nanodiamond (ND) particles an attractive alternative to molecular dyes drug‐delivery. A novel method is presented that can be used remote monitoring of chemical processes in biological environments based on changes photoluminescent (PL) nitrogen‐vacancy (NV) centers ND. The NV driven chemically by alternating the surface potential...
Background: Patient safety culture is critical for ensuring quality nursing care, yet its role not fully understood in various health care settings. Purpose: To explore the association between patient and nurses’ perceptions of provided hospitals Czech Republic. Methods: A correlation study was conducted with 535 nurses from 6 using questionnaires Hospital Survey on Safety Culture 2.0 Scale Perception Nursing Activities that Contribute to Care Quality. Results: Significant associations were...
Intestinal microbiota are considered to play an important role both in colorectal tumor development and the modulation of mucosal immunity. Studies on animals reared germ-free (GF, without intestinal microbiota) versus conventional (CV, with regular colonization bowel) conditions can aid clarifying influence bacteria carcinogenesis anticancer immune response. The capability environment modulate immunity not only at but also systemic level is still open question. In our study we found that,...
Glyco-coat changes on cancer cells due to aberrant glycosylation are potential targets for immune recognition through lectin-like receptors cells. These include natural killer (NK), CD8+ and CD4+ lymphocytes, all reported have, together with cytokines, important functions in antitumor immunity. The aim of this study was evaluate a possible role synthetic monodisperse multivalent neo-glycoconjugates, namely glycodendrimers, as new approach anticancer modulation carbohydrate-mediated...
Nanodiamonds have been gaining popularity in a variety of applications. These studies compare the cellular response to fluorescent, detonation, functionalized and drug-loaded nanodiamonds.
Efficient delivery of stabilized nucleic acids (NAs) into cells and release the NA payload are crucial points in transfection process. Here we report on fabrication a nanoscopic cellular carrier that is additionally combined with label-free intracellular sensor device, based biocompatible fluorescent nanodiamond particles. The sensing function engineered nanodiamonds by using nitrogen-vacancy color centers, providing stable non-blinking luminescence. device used for monitoring cells....
Abstract Natural killer receptor protein 1 (NKR-P1, a family of proteins), which is dimeric transmembrane predominantly on rat and murine natural cells, contains an extracellular motif related to calcium-dependent animal lectins. The domain architecture this the finding that its cross-linking with antibody results in activation cells make it promising candidate for function. We have expressed full-length NKR-P1 COS prepared soluble fragments by controlled proteolysis or expression truncated...
In this paper, new steps toward a better understanding and utilization of high-pressure high-temperature nanodiamonds (NDs) containing nitrogen-vacancy (NV) centers have been taken. NV–-related long-term luminescence oxygenated particles increased in comparison to plasma hydrogenated NDs' NV– luminescence. The optically detected electron spin resonance process can be also significantly affected by ND termination. For H-terminated the NV0 conversion energy is lower than excitation energy, so...
Glyco-coat changes on cancer cells due to aberrant glycosylation are potential targets for immune recognition through lectin-like receptors cells. These include natural killer (NK), CD8+ and CD4+ lymphocytes, all reported have, together with cytokines, important functions in antitumor immunity. The aim of this study was evaluate a possible role synthetic monodisperse multivalent neo-glycoconjugates, namely glycodendrimers, as new approach anticancer modulation carbohydrate-mediated...
Glycosphingolipid-enriched microdomains (GEM) are membrane entities that concentrate glycosylphosphatiolylinositol(GPI)-anchored, acylated and proteins important for immune receptor signaling. Using rat leukemic cell line RNK-16 we have initiated proteomic studies of in natural killer (NK) cells. Isolated plasma membranes were treated with Brij 58, or Nonidet-P40, sodium carbonate. Extracts separated by sucrose density gradient centrifugation into very light membrane, medium heavy fractions,...
Our study was aimed to characterize the phenotype and functional endpoints of local microwave hyperthermia (LHT, 42 °C) on tumor infiltrating spleen leukocytes. The effectiveness LHT applied into B16F10 melanoma-bearing C57/BL6 mice compared with anesthetized non-treated animals. Subpopulations leukocytes were analyzed using flow cytometry, cytotoxic activity splenocytes against syngeneic melanoma NK-sensitive YAC-1 cell lines evaluated in 51Cr-release assay. Similarly, vitro modification...
Rheumatoid arthritis is an autoimmunity leading to considerable impairment of quality life. N-acetyl glucosamine (GlcNAc) has been described previously as a potent modulator experimental in animal models and used for osteoarthritis treatment humans, praised its lack adverse effects. In this study we present comprehensive immunological analysis multivalent GlcNAc-terminated glycoconjugate (GC) application the collagen-induced (CIA) clinical outcome. We immunohistochemistry FACS describe...
Aberrant glycosylation, which impairs recognition capability of NK cells or modifies pattern target cells, is associated with cancer. Synthetic glycoconjugates (GCs), modulate cell increase the sensitivity tumor to therapy boost anti-cancer immune response. In current study, we employed N-acetyl-D-glucosamine-calix[4]arene (GN4C) as a modulator glycosylation represented by NK-92 line and fresh human cells. For first time, have demonstrated that calix[4]arene-based GC down-regulated...
Natural killer (NK) and T cells express a superfamily of proteins with structural features C-type lectins. Recombinantly prepared soluble form rat NKR-P1 (CD161) recognized carbohydrate GalNac GlcNac moieties. Ganglioside GM2 heparin related-IS oligosaccharides representing the high affinity ligands for this receptor, increased sensitivity targets killing by effectors isolated from blood spleen in vitro. Based on these results, we investigated vivo possible therapeutic effect IS carried...