A5051426276- Rabies epidemiology and control
- Immune Response and Inflammation
- Viral Infections and Vectors
- Monoclonal and Polyclonal Antibodies Research
- Toxoplasma gondii Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Biotin and Related Studies
- bioluminescence and chemiluminescence research
- Advanced Biosensing Techniques and Applications
- Molecular Communication and Nanonetworks
- Advanced Proteomics Techniques and Applications
- RNA regulation and disease
- interferon and immune responses
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- Protein purification and stability
- Machine Learning in Bioinformatics
- Poxvirus research and outbreaks
- Venomous Animal Envenomation and Studies
Université Paris Cité
2017-2020
Institut Pasteur
2016-2020
Sorbonne Paris Cité
2017-2020
Délégation Paris 7
2017-2020
Abstract The matrix (M) protein of wild isolates rabies virus such as Tha (M-Tha) was previously shown to be able interact with RelAp43, a the NF-κB family, and efficiently suppress NF-κB-dependent reporter gene expression, in contrast vaccine strain SAD. Here, we analyze mechanisms involved RelAp43-M interaction. We demonstrate that central part M-Tha, specific C-terminal region RelAp43 are required for this Four differences corresponding amino acid sequences M-Tha M-SAD crucial interaction...
At the crossroad between NF-κB and MAPK pathways, ternary complex composed of p105, ABIN2 TPL2 is essential for host cell response to pathogens. The matrix protein (M) field isolates rabies virus was previously shown disturb signaling induced by RelAp43, a close RelA/p65. Here, we investigated how M disturbs pathway in RelAp43-dependant manner potential involvement this mechanism. Using tandem affinity purification coupled with mass spectrometry approach, show that RelAp43 interacts...
Article18 September 2020Open Access Transparent process A combination of two human monoclonal antibodies cures symptomatic rabies Guilherme Dias de Melo orcid.org/0000-0003-0747-7760 Lyssavirus Epidemiology and Neuropathology Unit, Institut Pasteur, Paris, France Search for more papers by this author Florian Sonthonnax orcid.org/0000-0003-4777-7274 Sorbonne-Paris Cité, Cellule Université Paris-Diderot, Gabriel Lepousez Perception Memory Grégory Jouvion Experimental INSERM, Pathophysiology...
Phosphoprotein (P) and matrix protein (M) cooperate to undermine the immune response rabies virus (RABV) infections. While P is involved in modulation of Jak-Stat pathway through cytoplasmic retention interferon (IFN)-activated STAT1 (pSTAT1), M interacts with RelAp43-p105-ABIN2-TPL2 complex, efficiently inhibit nuclear factor-κB (NF-κB) pathway. Using transfections, protein-complementation assays, reverse genetics DNA ChIP, we identified a role control signaling pathway, synergy protein. In...
1st purification: a-FLAG (A2220; Sigma-Aldrich) 2nd GFP TRAP_A (ChromoTek)
This protocol aims to assess: - the interaction between two proteins harboring parts of gaussia luciferase (Glu1 and Glu2) in presence a third protein co-expressed rabies virus