A5058074531- Fungal and yeast genetics research
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Microbial Natural Products and Biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Fungal Biology and Applications
- Mycotoxins in Agriculture and Food
- Bacterial Genetics and Biotechnology
- Enzyme-mediated dye degradation
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Microbial Metabolism and Applications
- Plant Disease Resistance and Genetics
- Viral Infectious Diseases and Gene Expression in Insects
- Endoplasmic Reticulum Stress and Disease
- Bacteriophages and microbial interactions
- Photosynthetic Processes and Mechanisms
- Glycosylation and Glycoproteins Research
- Protist diversity and phylogeny
- Monoclonal and Polyclonal Antibodies Research
- Microbial Community Ecology and Physiology
- CRISPR and Genetic Engineering
- Antifungal resistance and susceptibility
- Transgenic Plants and Applications
Leiden University
2014-2024
Biotechnology Institute
2015
Innsbruck Medical University
2014
Cancer Genomics Centre
2010-2012
Food & Nutrition
1997-2008
Pension Fund for Care and Well-Being
2005
Food Research Institute
1998-2003
DDL Diagnostic Laboratory
1985-1994
University of Birmingham
1991
Netherlands Organisation for Applied Scientific Research
1989
The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for production of enzymes and organic acids, particularly citric acid. We sequenced 33.9-megabase genome A. CBS 513.88, ancestor currently used enzyme strains. A high level synteny was observed with other aspergilli sequenced. Strong function predictions were made 6,506 14,165 open reading frames identified. detailed description components protein secretion pathway striking differences in hydrolytic...
The fungus Fusarium oxysporum f. sp. radicis-lycopersici is the causal agent of tomato foot and root rot disease. green fluorescent protein (GFP) was used to mark this in order visualize analyze colonization infection processes vivo. Transformation F very efficient gfp expression stable for at least nine subcultures. Microscopic analysis transformants revealed homogeneity signal, which clearly visible hyphae as well chlamydospores conidia. To our knowledge, first report shown. transformation...
ABSTRACT Filamentous fungi are the cause of serious human and plant diseases but also exploited in biotechnology as production platforms. Comparative genomics has documented their genetic diversity, functional systems biology approaches under way to understand functions interaction fungal genes proteins. In these approaches, gene usually inferred from deletion or overexpression mutants. However, studies at extreme points give only limited information. Moreover, many use metabolism-dependent...
Abstract Background Filamentous fungi such as Aspergillus niger have a high capacity secretory system and are therefore widely exploited for the industrial production of native heterologous proteins. However, in most cases yields non-fungal proteins significantly lower than those obtained fungal One well-studied bottleneck appears to be result mis-folding ER during early stages secretion, with related stress responses host, including unfolded protein response (UPR). This study aims at...
Abstract Bacterial-fungal interactions influence microbial community performance of most ecosystems and elicit specific behaviours, including stimulating specialised metabolite production. Here, we use a co-culture experimental evolution approach to investigate bacterial adaptation the presence fungus, using simple model bacterial-fungal encompassing bacterium Bacillus subtilis fungus Aspergillus niger . We find in one evolving population that B. was selected for enhanced production...
ABSTRACT Nematophagous fungi are soil-living that used as biological control agents of plant and animal parasitic nematodes. Their potential could be improved by genetic engineering, but the lack information about molecular background infection has precluded this development. In paper we report a subtilisin-like extracellular serine protease designated PII is an important pathogenicity factor in common nematode-trapping fungus Arthrobotrys oligospora . The transcript was not detected during...
Pycnoporus cinnabarinus laccase lac1 gene was overexpressed in Aspergillus niger , a well‐known fungal host producing large amount of homologous or heterologous enzymes for industrial applications. The corresponding cDNA placed under the control glyceraldehyde‐3‐phosphate dehydrogenase promoter as strong and constitutive promoter. signal peptide glucoamylase preprosequence A. used to target secretion. Both peptides directed secretion into culture medium an active protein, but allowed 80‐fold...