A5090783738- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Plant biochemistry and biosynthesis
Technical University of Denmark
2019-2020
Abstract Fungal polyketides display remarkable structural diversity and bioactivity, therefore the biosynthesis engineering of this large class molecules is therapeutically significant. Here, we successfully recode, construct characterize biosynthetic pathway bikaverin, a tetracyclic polyketide with antibiotic, antifungal anticancer properties, in S. cerevisiae . We use green fluorescent protein (GFP) mapping strategy to identify low expression Bik1 (polyketide synthase) as major bottleneck...
Abstract Fungal polyketides display remarkable structural diversity and bioactivity, therefore the biosynthesis engineering of this large class molecules is therapeutically significant. Here, we successfully recoded, constructed characterized biosynthetic pathway formation bikaverin, a tetracyclic polyketide with antibiotic, antifungal anticancer properties, in S. cerevisiae . We used green fluorescent protein (GFP) tagging strategy to identify low expression Bik1 (polyketide synthase) as...