A5033420882- Antibiotic Resistance in Bacteria
- Liver physiology and pathology
- Plant Molecular Biology Research
- Immunotherapy and Immune Responses
- Plant Reproductive Biology
- Cancer, Hypoxia, and Metabolism
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Bacterial Genetics and Biotechnology
- Drug Transport and Resistance Mechanisms
- Light effects on plants
- Cancer Mechanisms and Therapy
- Cancer Diagnosis and Treatment
- T-cell and B-cell Immunology
- Plant tissue culture and regeneration
- Amino Acid Enzymes and Metabolism
- Gastric Cancer Management and Outcomes
- Glycosylation and Glycoproteins Research
- Metastasis and carcinoma case studies
- Postharvest Quality and Shelf Life Management
- Escherichia coli research studies
- Cancer Research and Treatments
- Analytical Chemistry and Chromatography
- Bacterial biofilms and quorum sensing
- ATP Synthase and ATPases Research
Osaka University
2016-2025
Osaka Research Institute of Industrial Science and Technology
2015-2025
Itoham Foods (Japan)
2021-2025
Osaka University of Pharmaceutical Sciences
2010-2023
Kyushu University
2023
University of Teacher Education Fukuoka
2003-2017
Takeda (Japan)
2013-2017
Hokkaido University
2017
National Hospital Organization Kochi National Hospital
2016
Shizuoka Cancer Center
2010-2015
Gut microbiota has been reported to be closely related host energy metabolism and immunity, thus influence the development progression of various human diseases. To date, gut microbial metabolites such as short-chain fatty acids, defensins, cathelicidins, lactoferrin in feces have investigated biomarkers associated with disease conditions. In this review, we introduce intestinal fecal pH, which is relatively easy rapid measure compared composition its metabolites. particular, review presents...
Human melanoma cells can process the MAGE-1 gene product and present processed nonapeptide EADPTGHSY on their major histocompatibility complex class I molecules, HLA-A1, as a determinant for cytolytic T lymphocytes (CTLs). Considering that autologous antigen presenting (APCs) pulsed with synthetic might, therefore, be immunogenic, patients whose tumor express who are HLA-A1+ were immunized vaccine made of cultured APCs nonapeptide. Analyses nature in vivo host immune response to revealed...
AcrB is the major multidrug exporter in Escherichia coli. Although several substrate-entrances have been identified, specificity of these various transport paths remains unclear. Here we present evidence for a substrate channel (channel 3) from central cavity trimer, which connected directly to deep pocket without first passing switch-loop and proximal . Planar aromatic cations, such as ethidium, prefer 3 channels 1 2. The efflux through increases by targeted mutations not competition with...
<i>Objective:</i> Fascin, an actin-bundling protein that is found in membrane ruffles, microspikes, and stress fibers, induces protrusions increases cell motility various transformed cells. The expression of fascin epithelial neoplasms has been described only recently, its role gastric cancer still unknown. <i>Methods:</i> Paraffin sections carcinoma from 214 patients were immunohistochemically investigated using monoclonal antifascin antibody. Staining more than 5%...
Sex determination in cucumber (Cucumis sativus L.) plants is genetically controlled by the F and M loci. These loci interact to produce three different sexual phenotypes: gynoecious (M-F-), monoecious (M-ff), andromonoecious (mmff). Gynoecious more ethylene than do plants. We found that levels of production accumulation CS-ACS2 mRNA did not differ from those were lower measured Ethylene inhibited stamen development cucumbers but ones. Furthermore, caused substantial increases CS-ETR2,...
Despite being fundamentally important and having direct therapeutic implications, the functional genomics of clinical isolates multidrug-resistant (MDR) pathogens is often impeded by lack genome-editing tools. Here, we report establishment a highly efficient, in situ technique applicable environmental prototypic MDR pathogen P. aeruginosa harnessing endogenous type I-F CRISPR-Cas systems. Using this approach, generate various reverse mutations an epidemic genotype, PA154197, identify...
Drug efflux pumps transport antimicrobial agents out of bacteria, thereby reducing the intracellular concentration, which is associated with intrinsic and acquired bacterial resistance to these antimicrobials. As genome analysis has advanced, many drug pump genes have been detected in genomes species. In addition resistance, are involved various essential physiological functions, such as adaptation hostile environments, toxin metabolite efflux, biofilm formation quorum sensing. Gram-negative...
It has been reported that ethylene production by cucumber plants is strongly related to the sex expression of their flowers. also shown both CS-ACS2 gene and evolution are much greater in gynoecious than monoecious ones. To investigate action mechanism induction femaleness flowers, we isolated three ethylene-receptor-related genes, CS-ETR1, CS-ETR2 CS-ERS, from (Cucumis sativus L.) plants. Of these CS-ERS mRNA accumulated more substantially shoot apices those one. Their patterns correlated...
Ethylene plays a key role in sex determination of cucumber flowers. Gynoecious shoots produce more ethylene than monoecious shoots. Because cucumbers both male and female flower buds the shoot apex because relative proportions flowers vary due to growing conditions, question arises as whether regulation biosynthesis each bud determines flower. Therefore, expression 1-aminocyclopropane-1-carboxylic acid synthase gene, CS-ACS2, was examined at different stages development. The results revealed...
Sharp-headed and globular-headed trichomes are found on the surface of cucumber (Cucumis sativus L.) cotyledons. Most sharp-headed consist three cells. Toluidine blue O stains but not trichomes. The effect continuous ultraviolet-B (UV-B; 290-320 nm) irradiation cotyledons was examined with respect to two trichome types. Continuous UV-B induced cell division at or under basal part trichomes, resulting in an increase number layers from six. In parallel, area stained by toluidine expanded...
ABSTRACT NlpE, an outer membrane lipoprotein, functions during envelope stress responses in Gram-negative bacteria. In this study, we report that overproduction of NlpE increases multidrug and copper resistance through activation the genes encoding AcrD MdtABC efflux pumps Escherichia coli .
ABSTRACT The AcrAB-TolC system in Escherichia coli is an intrinsic RND-type multidrug efflux transporter that functions as a tripartite complex of the inner membrane AcrB, outer channel TolC, and adaptor protein AcrA. Although crystal structures each component this have been elucidated, structure whole has not solved. available shown AcrB TolC function trimers, but number AcrA molecules now under debate. Disulfide chemical cross-linking experiments indicated stoichiometry AcrB-AcrA-TolC...
Abstract 1,3 : 2,4-Di-O-benzylidene-d-sorbitol (d-DBS) can cause gelation of organic solvents a wide range polarity into gel state. The aggregated structures d-DBS/organic solvent systems were studied, mainly by using IR, UV, and circular dichroism (CD) spectroscopy. In the case racemate Dl-DBS, no state was formed. IR spectra d-DBS Dl-DBS in solid state, intensities νOH νCO absorption found to be larger than those Dl-DBS. It is assumed that hydrogen bonding formed between an acetal oxygen...
Most trichomes on the surface of cucumber (Cucumis sativus L.) cotyledons consist three cells. We previously showed that continuous UV-B (290-320 nm) irradiation induces rapid cellular expansion and accumulation polyphenolic compounds, possibly stress lignin, in epidermal cells around these trichomes.(1)) To examine mechanism UV-B-induced to determine which step is stimulated by lignin synthesis pathway, we investigated relative DNA contents cells, including trichomes, enzyme activity gene...
Several putative and proven drug efflux pumps are present in Escherichia coli. Because many such have overlapping substrate spectra, it is intriguing that bacteria, with their economically organized genomes, harbour large sets of multidrug genes. To understand how bacteria utilize these multiple pumps, important to elucidate the process pump expression regulation. The aim this study was determine a regulator organism. We screened genomic library E. coli for genes decreased susceptibility...