A5038883582- Spine and Intervertebral Disc Pathology
- Scoliosis diagnosis and treatment
- Musculoskeletal pain and rehabilitation
- Plant Toxicity and Pharmacological Properties
- Spaceflight effects on biology
- Spinal Fractures and Fixation Techniques
- Osteoarthritis Treatment and Mechanisms
- Bee Products Chemical Analysis
- Space Exploration and Technology
- Anesthesia and Neurotoxicity Research
- Genetics, Aging, and Longevity in Model Organisms
- Anesthesia and Pain Management
- Anesthesia and Sedative Agents
- Sesquiterpenes and Asteraceae Studies
- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Intensive Care Unit Cognitive Disorders
- Natural product bioactivities and synthesis
- Lipid Membrane Structure and Behavior
- Pregnancy-related medical research
- Orthopaedic implants and arthroplasty
- Biological Activity of Diterpenoids and Biflavonoids
- Synthesis and biological activity
Shriners Hospitals for Children - Canada
2018-2025
McGill University
2017-2025
Concord Hospital
2024
McGill University Health Centre
2017-2020
Orthopaedic Research
2020
Curcumin and o-Vanillin cleared senescent intervertebral disc (IVD) cells reduced the senescence-associated secretory phenotype (SASP) associated with inflammation back pain. Cells from degenerate non-mildly-degenerate human IVD were obtained organ donors patients undergoing surgery for low Gene expression of senescence SASP markers was evaluated by RT-qPCR in isolated cells, protein senescence, proliferation, apoptotic immunocytochemistry (ICC). The levels factors enzyme-linked...
Cellular senescence is a contributor to intervertebral disc (IVD) degeneration and low back pain. Here, we found that RG-7112, potent mouse double-minute two protein inhibitor, selectively kills senescent IVD cells through apoptosis. Gene expression pathway analysis was used compare the functional networks of genes affected by pure synthetic senolytic with o-Vanillin natural anti-inflammatory senolytic. Both gene network related cell death survival. O-Vanillin also cycle progression as well...
Toll-like receptors (TLR) are activated by endogenous alarmins such as fragmented extracellular matrix compounds found in the degenerating disc. TLRs regulate cytokine, neurotrophin, and protease expression human disc cells vitro, thus control key factors degeneration. However, whether TLR activation leads to degenerative changes intact discs is unclear. Nucleus pulposus (NP) isolated from non-degenerating increase IL-1β nerve growth factor gene following treatment with Pam2CSK4 (TLR2/6...
Facet joint osteoarthritis (OA) is prevalent in patients with adolescent idiopathic scoliosis (AIS). The most pronounced OA presents above and below the curve's apex where intervertebral rotation greatest. This indicates that facet implicated potentially contributes to AIS progression. impacts both cartilage bone we have previously demonstrated an association between lower quality more severe joints. study aimed further investigate molecular mechanisms underlying cartilage–bone crosstalk...
Pain in lower back is a common condition reported by astronauts, both during and after space missions. Investigating the alterations spine mechanisms driving these changes essential for deeper understanding of how microgravity impacts human spine. This knowledge could also open pathways therapeutic or preventive interventions. Nevertheless, there limited evidence regarding intervertebral discs (IVDs) due to travel. In this study, 2 astronauts were enrolled Before flight, magnetic resonance...
Autologous NP cell implantation is a potential therapeutic avenue for intervertebral disc (IVD) degeneration.However, monolayer expansion of cells isolated from surgical samples may negatively impact matrix production by way dedifferentiation.Previously, we have used continuous culture system to successfully preserve chondrocyte phenotype.In this work, hypothesised that could also nucleus pulposus (NP) phenotype.We confirmed serial passaging drove dedifferentiation significantly decreasing...
Adolescent idiopathic scoliosis (AIS) is a poorly understood deformity of the thoracolumbar spine which affects intervertebral discs (IVDs) and articular facet joints. The knowledge concerning joints in this context very limited, although joint degeneration known contributor back pain. In study, comprehensive investigation was performed to characterize chondrocytes extracellular matrix within scoliotic spine. Surgically removed tissues were collected from patients undergoing spinal...
Facet joint osteoarthritis is prevalent in young patients with adolescent idiopathic scoliosis (AIS) and might contribute to back pain. Toll-like receptors (TLR) have been linked cartilaginous tissue degeneration but their involvement facet AIS still unknown. We compared baseline gene expression levels of TLRs -1, -2, -4, -6 scoliotic non-scoliotic chondrocytes found higher significantly TLR2 levels. Furthermore, TLR correlated strongly inflammatory catabolic markers not chondrocytes....
Purpose: Pain is an understudied physiological effect of spaceflight. Changes in inflammatory and tissue degradation markers are often associated with painful conditions. Our aim was to evaluate the changes deterioration after a short-term Patients Methods: Plasma levels for systemic inflammation degeneration were assessed two astronauts before within 24 h 17-day Axiom Space AX-1 mission. Results: After spaceflight, C-reactive protein (CRP) reduced both astronauts, while INFγ, GM-CSF, TNFα,...
Abstract Delirium is a complex neurocognitive disorder characterized by an acute disturbance in attention, awareness, and perception. It dangerous syndrome that independently associated with higher rates of morbidity mortality, inpatient complications, predictor long‐term cognitive dysfunction. Although delirium can occur persons all ages, the prevalence among impact on older adults particularly significant. Current gold standard approaches for include treating medical precipitants...