A5004460248- Microbial Inactivation Methods
- Transgenic Plants and Applications
- Virus-based gene therapy research
- Cellular transport and secretion
- RNA Interference and Gene Delivery
- Toxin Mechanisms and Immunotoxins
- Immunotherapy and Immune Responses
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- Advanced biosensing and bioanalysis techniques
- Anesthesia and Sedative Agents
- Lipid Membrane Structure and Behavior
- Antioxidant Activity and Oxidative Stress
- Health Systems, Economic Evaluations, Quality of Life
- Anesthesia and Neurotoxicity Research
- Microfluidic and Bio-sensing Technologies
- Calcium signaling and nucleotide metabolism
- Bacillus and Francisella bacterial research
- Retinoids in leukemia and cellular processes
- Lysosomal Storage Disorders Research
- Phytochemicals and Antioxidant Activities
- Consumer Attitudes and Food Labeling
- Cardiac pacing and defibrillation studies
- BRCA gene mutations in cancer
- Effects and risks of endocrine disrupting chemicals
University of Oslo
1996-2017
Inovio Pharmaceuticals (United States)
2006-2012
University Hospital Bonn
2009
Maulana Azad Medical College
2009
Norwegian Cancer Society
2000-2002
Cancer Registry of Norway
2000
In this study we take advantage of recently developed methods using J774 macrophages to prepare enriched fractions early endosomes, late dense lysosomes, as well phagosomes different ages enclosing 1-μm latex beads investigate the steady state distribution and trafficking lysosomal enzyme activity between these organelles. At cells appear possess four cellular structures, in addition phagolysosomes, where acid hydrolases were concentrated. The first site hydrolase concentration was which...
We are evaluating the use of electroporation (EP) to deliver a novel DNA vaccine, p.DOM-PSMA(27). This vaccine encodes domain (DOM) fragment C tetanus toxin induce CD4(+) T cell help, fused tumor-derived epitope from prostate-specific membrane antigen (PSMA) for in HLA-A2(+) patients with recurrent prostate cancer. report on safety and tolerability antibody response DOM as first indication effect EP patients. In this open label phase I/II, two-arm, dose escalation trial was delivered either...
ABSTRACT Although endosomal proteolysis has been reported (e.g. for peptide hormones and lysosomal enzymes), lysosomes are believed to be the main site of degradation in endocytic pathway. We have studied separate roles prelysosomal organelles ovalbumin J774 cells. The was labelled with 125I-tyramine cellobiose (125I-TC-ova). products formed from this probe trapped at formation. To efficiently we allowed cells endocytose a pulse colloidal gold particles complexed ovalbumin. By combining...
Abstract The mechanisms by which in vivo electroporation (EP) improves the potency of i.m. DNA vaccination were characterized using hepatitis C virus nonstructural (NS) 3/4A gene. Following a standard injection with or without EP, plasmid levels peaked immediately at site and decreased 4 logs first week. In EP did not promote persistence and, depending on dose, was cleared almost after 60 days. imaging immunohistochemistry revealed that protein expression restricted to despite detection...
We report on the immunogenicity and clinical effects in a phase I/II dose escalation trial of DNA fusion vaccine patients with prostate cancer. The encodes domain (DOM) from fragment C tetanus toxin linked to an HLA-A2-binding epitope prostate-specific membrane antigen (PSMA), PSMA(27-35). evaluated effect intramuscular vaccination without or electroporation (EP) potency. Thirty-two HLA-A2(+) were vaccinated monitored for immune responses follow-up period 72 weeks. At week 24, cross-over...
We describe novel biochemical and electron microscopy assays to investigate <i>in vitro</i> fusion of latex bead phagosomes with three different endocytic organelle fractions from J774 macrophages. After formation, early fuse avidly late endosomes for a longer period time lysosomes, but they subsequently become fusion-incompetent. The early, not late, all could be significantly stimulated by Rab5. In contrast other cell types investigated, this Rab is uniquely enriched on both in Moreover,...
The development of methods for specific delivery therapeutic genes into target tissues is an important issue the further progress in vivo gene therapy. In this article we report on a novel technology, named photochemical transfection, to use light direct precise desired location. technology makes photosensitizing compounds that localize mainly membranes endosomes and lysosomes. On illumination these membrane structures will be destroyed, releasing endocytosed DNA cell cytosol. Using green...
Abstract Injection of plasmid DNA encoding antigens into rodents followed by electroporation improved the immune response when compared with injection without (Widera et al. J Immunol 2000;164:4635–40; Zucchelli Virol 2000;74:11598–607; Kadowaki al . Vaccine 2000;18:2779–88). The present study describes extension this technology to farm animals, injecting mycobacterial (MPB70, Ag85B and Hsp65) muscles goats cattle using two different types electrodes, both allowing delivery at site...
PXR activators are used to treat pruritus in chronic inflammatory liver diseases such as primary biliary cirrhosis (PBC). The aims of this study were determine whether could have an additional benefit inhibiting inflammation the liver, and cyclosporin A - which more effectively prevents PBC recurrence transplanted patients than FK506 is a activator. In SJL/J mice (which constitutively high levels hepatic portal tract cell recruitment), feeding activator inhibited inflammation, TNFalpha...
Intake of fruits and berries may lower blood pressure (BP), most probably due to the high content polyphenols. In present study, we tested whether consumption two polyphenol-rich juices could BP. a randomised, double-blinded, placebo-controlled trial 12 weeks, 134 healthy individuals, aged 50-70 years, with normal range BP (130/85-139/89 mmHg, seventy-two subjects) or stage 1-2 hypertension (140/90-179/109 sixty-two subjects), were included. They consumed 500 ml/d one either (1) commercially...
LTX 315 is an oncolytic peptide with potent immunological properties. In the present study, we demonstrate that intratumoral treatment LTX-315 resulted in a complete regression and systemic immune response rat fibrosarcoma model. The was T-cell dependent, also abscopal effect as demonstrated by of distal non-treated lesions. Significant infiltration CD8+ T cells observed both treated lesions, shown immunohistochemical flow cytometric analysis. rapidly killed vitro lytic mode action followed...
Background Most non-viral gene therapy vectors deliver transgenes into cells through the endocytic pathway. Lack of escape from vesicles in many cases constitutes a major barrier for delivery functional gene. We have developed new technology named photochemical internalisation (PCI) to achieve light-inducible cytosolic transgene. The is based on treatment employing photosensitisers localised vesicles. In this work mechanisms involved PCI-mediated transfection (photochemical transfection)...
Antibodies are useful for the treatment of a variety diseases. We here demonstrate that mouse muscle produced monoclonal antibodies (mAb) after single injection immunoglobulin genes as plasmid DNA. In vivo electroporation greatly enhanced antibody production. For chimeric antibodies, levels 50–200 ng mAb/ml serum were obtained but declined 7–14 days due to an immune response against xenogeneic parts antibody. By contrast, fully persisted in at least 7 months. mAb by had correct structure,...
Background Injection of DNA encoding exogenic proteins into muscle tissue combined with electroporation often results in a transient increase the encoded protein concentration and blood. The reduction is normally due to an immune response against but other factors may also be involved. How various parameters affect kinetics syngenic studied relation damage after electroporation-mediated transfer muscle. Methods Electroporation was applied mouse quadriceps rat tibialis anterior muscles...
Preservation of human blood cells for DNA damage analysis with the comet assay conventionally involves isolation mononuclear by centrifugation, suspension in freezing medium and slow to −80 °C—a laborious process. A recent publication (Al‐Salmani et al . Free Rad Biol Med 2011; 51: 719–725) describes a simple method which small volumes whole are frozen −20 or °C; on subsequent thawing, is performed, no indication elevated strand breakage resulting from rapid freezing. However, leucocytes...
Abstract Background Genes encoding non‐self proteins may be injected into skeletal muscles in vivo to obtain induction of cellular and humoral immune responses against the encoded antigens (DNA vaccination). Bone marrow derived professional antigen‐presenting cells (APCs) play a key role immunity by DNA vaccination. In present work we have investigated whether APCs are transfected injection muscle. Methods green fluorescent protein (GFP) was rat mouse limb muscle followed electroporation....