A5026030636- CRISPR and Genetic Engineering
- Genetics, Bioinformatics, and Biomedical Research
- Bacteriophages and microbial interactions
- RNA Interference and Gene Delivery
- Herpesvirus Infections and Treatments
- Biomedical and Engineering Education
- Career Development and Diversity
- Poxvirus research and outbreaks
- Virus-based gene therapy research
- Plant Virus Research Studies
- Animal Disease Management and Epidemiology
- Genomics and Phylogenetic Studies
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Teaching and Learning Programming
- Educational Strategies and Epistemologies
- Force Microscopy Techniques and Applications
- Interdisciplinary Research and Collaboration
- Cellular Mechanics and Interactions
- Grit, Self-Efficacy, and Motivation
- Cellular transport and secretion
- Vector-Borne Animal Diseases
McDaniel College
2007-2022
National Institutes of Health
2001-2007
National Institute of Allergy and Infectious Diseases
2005-2007
Department of Embryology
2001-2002
Carnegie Institution for Science
2000-2002
Carnegie Observatories
2001-2002
Johns Hopkins University
2000-2002
National Human Genome Research Institute
2001
University of Maryland, Baltimore County
1999
Short interfering RNAs (siRNAs) are double-stranded of ≈21–25 nucleotides that have been shown to function as key intermediaries in triggering sequence-specific RNA degradation during posttranscriptional gene silencing plants and interference invertebrates. siRNAs a characteristic structure, with 5′-phosphate/3′-hydroxyl ends 2-base 3′ overhang on each strand the duplex. In this study, we present data synthetic can induce gene-specific inhibition expression Caenorhabditis elegans cell lines...
Genomics is not only essential for students to understand biology but also provides unprecedented opportunities undergraduate research. The goal of the Education Partnership (GEP), a collaboration between growing number colleges and universities around country Department Biology Genome Center Washington University in St. Louis, provide such research opportunities. Using versatile curriculum that has been adapted many different class settings, GEP undergraduates undertake projects bring...
There is widespread agreement that science, technology, engineering, and mathematics programs should provide undergraduates with research experience. Practical issues limited resources, however, make this a challenge. We have developed bioinformatics project provides course-based experience for students at diverse group of schools offers the opportunity to tailor local curriculum institution-specific student needs. assessed both attitude knowledge gains, looking insights into how respond...
Previous studies indicated that the vaccinia virus D10 protein, which is conserved in all sequenced poxviruses, participates rapid turnover of host and viral mRNAs. contains a motif present family Nudix/MutT enzymes, subset has been shown to enhance mRNA eukaryotic cells through cleavage 5' cap (m7GpppNm-). Here, we demonstrate purified recombinant fusion protein possesses an intrinsic activity liberates m7GDP from capped RNA substrates. Furthermore, point mutations abolished decapping...
Vaccinia virus (VACV) encodes enzymes that cap the 5' end of viral mRNAs, which enhances their stability and translation. Nevertheless, recent studies demonstrated VACV D10 protein (VACV-WR_115) decaps mRNA, an enzymatic activity not previously shown to be encoded by a virus. The decapping is dependent on Nudix hydrolase motif also present in D9 (VACV-WR_114), shares 25% sequence identity with D10. Here, we showed purified recombinant fusion mRNA this was abolished point mutations motif....
In their 2012 report, the President's Council of Advisors on Science and Technology advocated “replacing standard science laboratory courses with discovery-based research courses”—a challenging proposition that presents practical pedagogical difficulties. this paper, we describe our collective experiences working Genomics Education Partnership, a nationwide faculty consortium aims to provide undergraduates experience in genomics through scheduled course (a classroom-based undergraduate...
A hallmark of the research experience is encountering difficulty and working through those challenges to achieve success. This ability essential being a successful scientist, but replicating such in teaching setting can be difficult. The Genomics Education Partnership (GEP) consortium faculty who engage their students genomics Course-Based Undergraduate Research Experience (CURE). Students participate genome annotation, generating gene models using multiple lines experimental evidence. Our...
The D9 and D10 proteins of vaccinia virus are 25% identical to each other, contain a mutT motif characteristic nudix hydrolases, conserved in all sequenced poxviruses. Previous studies indicated that overexpression and, lesser extent, decreased the levels capped mRNAs their translation products. Here, we further characterized protein showed only trace amounts associated with purified virions it is expressed exclusively at late times after infection. A viable deletion mutant (vdeltaD10)...
The Genomics Education Partnership (GEP) engages students in a course-based undergraduate research experience (CURE). To better understand the student attributes that support success this CURE, we asked about their attitudes using previously published scales measure epistemic beliefs work and science, interest grit. We found, general, bring with them into classroom contribute to two outcome measures, namely, learning as assessed by pre- postquiz perceived self-reported benefits. While GEP...
The giant Mimivirus is a member of the nucleocytoplasmic large DNA viruses (NCLDV), group diverse that contain double-stranded (dsDNA) genomes replicate primarily in eukaryotic hosts. Two members NCLDV, Vaccinia Virus (VACV) and African Swine Fever (ASFV), both synthesize Nudix enzymes have been shown to decap mRNA, process thought accelerate viral host mRNA turnover promote shutoff protein synthesis. encodes two its genome, denoted as L375 L534. Importantly, exhibits sequence similarity...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTToxicology for Middle School: The Effect of Common Substances on DaphniaRuth N. Russo and Susan Parrish Cite this: J. Chem. Educ. 1995, 72, 1, 49Publication Date (Print):January 1995Publication History Received3 August 2009Published online1 January 1995Published inissue 1 1995https://pubs.acs.org/doi/10.1021/ed072p49https://doi.org/10.1021/ed072p49research-articleACS PublicationsRequest reuse permissionsArticle Views1516Altmetric-Citations3LEARN...
Abstract The giant Mimivirus is a member of the nucleocytoplasmic large DNA viruses (NCLDV), group diverse that contain double-stranded (dsDNA) genomes replicate primarily in eukaryotic hosts. Two members NCLDV, Vaccinia Virus (VACV) and African Swine Fever (ASFV), both synthesize Nudix enzymes have been shown to decap mRNA, process thought accelerate viral host mRNA turnover promote shutoff protein synthesis. encodes two its genome, denoted as L375 L534. Importantly, exhibits sequence...