The novel approach for deposition of iron oxide nanoparticles with narrow size distribution supported on different sized graphene was reported. Two samples distributions (0.5 to 7 μm and 1 3 μm) were selectively prepared, the influence flake mitochondrial activity L929 WST1 assay in vitro study also evaluated. Little reduction GO-Fe3O4 broader observed. pristine GO highest concentrations reduced significantly. For narrower distribution, best biocompatibility noticed at concentration 12.5...

In the present study, analysis of epithelial cells derived from various sources was undertaken, beginning mammary gland tissue through primary cultures and their subsequent passages. The objective study comparative stage in which obtained individuals different lactation cycles disparate phases cell culture growth are most suitable for morphological research gene expression activity. bovine passages were identified morphologically using immunocytochemical methods. After positive...

Hexagonal boron nitride is often referred to as white graphene. This a 2D layered material, with structure similar It has gained many applications in cosmetics, dental cements, ceramics etc. also used medicine, drug carrier graphene or oxide. Here we report that this material can be exfoliated two steps: chemical treatment (via modified Hummers method) followed by the sonication treatment. Afterwards, surface of obtained efficiently functionalized gold nanoparticles. The mitochondrial...

Nanoparticles (e.g., graphene oxide, oxide-Fe3O4 nanocomposite or hexagonal boron nitride) loaded with anti-cancer drugs and targeted at cancerous cells allowed researchers to determine the most effective in vitro conditions for anticancer treatment. For this reason, main propose of present study was effect oxide (GO) iron (Fe3O4) nanoparticles (GO-Fe3O4) covalently (c-GO-Fe3O4-HCPT) non-covalently (nc-GO-Fe3O4-HCPT) conjugated hydroxycamptothecin (HCPT) presence a rotating magnetic field...

Abstract To induce the water solubility of hexagonal boron nitride (h-BN), we exfoliated and functionalized bulk h-BN with hydroxyl groups (h-BN-OH-n). Short-term studies showed that h-BN-OH-n induced low cytotoxicity in different models: insect haemocytes ( vivo ), human erythrocytes mouse fibroblasts vitro ). We also demonstrated Alexa Fluor 647-h-BN-OH-n administered topically to insects passed through cuticle barrier was phagocytosed by haemocytes. Nanoflakes did not affect haemocyte...

<ns3:p>The final results of racing pigeons in races are affected by numerous genetic and non-genetic factors. The individual traits determined, inter alia, genes, the result selection to improve speed, spatial orientation, endurance. Within LDHA gene structure STR polymorphism were described, thus aim study was association analysis between (TTTAT)3-5 microsatellite performance using HRM-based genotyping method. within intron 6 resulted identification five genotypes with highest frequencies...

This study presents the procedure of deriving a compartmental model (CM) based on an analysis obtained from computational fluid dynamics (CFD) bioreactor. The CM is composed two parts, structural (that takes into account architecture mathematical model), and parametric part (which contains extrinsic parameters model). branches containing set perfectly mixed continuous stirred-tank reactors (CSTRs) in configuration that matches bioreactor’s flow patterns. Therefore, this work’s main objective...

Polymeric carbon nitride (PCN), which demonstrates unique properties, has been widely explored, mostly in photocatalysis; however, the evaluation of its biocompatibility is still needed. Herein, cytocompatibility PCN with different lateral size distributions (A-PCN 160 nm, B-PCN 20 and C-PCN 10 nm dominating sizes) was investigated. The viability three cell lines (L929, MCF-7, HepG2) determined using counting kit-8 (CCK-8), neutral red uptake (NRU), lactate dehydrogenase (LDH) leakage...

Since the effect of MFs (magnetic fields) on various biological systems has been studied, different results have obtained from an insignificant weak disruption circadian clock system. On other hand, magnetic fields, electromagnetic or electric fields are used in medicine. The presented study was conducted to determine whether a low-frequency RMF (rotating field) with field parameters could evoke cellular response vitro and is possible modulate response. metabolic activity, ROS Ca

The effect of hybrid material—graphene flakes with Fe 3 O 4 nanospheres (GO-Fe ), graphene oxide (GO) and magnetite (Fe ) in rotating magnetic field on mammalian cells metabolism has been studied. Several reports shown that exposure to may have influence cellular membrane permeability. Thus, the aim presented study was determine response L929 fibroblast nanomaterials for 8-h experiment. GO had tendency adsorb proteins, thus cell decreased mechanism enhanced by impact field. highest reduction...

The aim of this study was to relate polymorphic variants CYP19 , CYP21 and ER1 genes reproductive traits in 472 Polish Holstein-Friesian cows. High frequencies one the homozygous genotypes were found. / Sna BI AA homozygotes not identified. In first third lactation, an average calving-to-conception interval (CLVC) cows GG genotype significantly shorter ( P ≤ 0.05) than heterozygous Bgl I genotype, CLVC observed compared heterozygotes whereas longer ones. It also found that characterized by...

The relationships between the SNPs of cytochrome P450 gene (CYP19/PvuII), steroid 21-hydroxylase (P450c21) (CYP21/HpaII), 2 polymorphic sites estrogen receptor alpha (ER1/BglI and ER1/SnaBI), milk production traits Black-and-White cattle were analyzed. A total 472 cows included in study genotyped using PCR-RFLP. frequencies alleles for as follows: 0.923 - CYP19A, 0.077 CYP19B, 0.042 ER1/BglIA, 0.958 ER1/BglIG. For ER1/SnaBI, frequency allele was 0.960 that G 0.040. In CYP21, all AA (no...

Abstract The study was aimed at investigating the effect of Fe 3 O 4 hybrid deposited on graphene oxide (GO-Fe ) relative viability and DNA integrity. properties GO-Fe were analyzed using a transmission electron microscopy (TEM), X-ray diffraction technique (XRD) thermal gravimetric method (TGA), while efficiency covalent functionalization with iron nanospheres determined by Fourier transform infrared spectroscopy (FT-IR). L929 MCF-7 cell lines selected to analyze biocompatibility...

The aim of this study was to apply boosted classification trees dystocia detection in dairy cattle and indicate its most important predictors. A total 1742 calving records were used. Proportions correctly detected difficult easy calvings a test set 75.0%, 92.0% 77.3%, for heifers multiparous cows, respectively. key predictors difficulty were: pregnancy length, body condition score index, age, proportion Holstein-Friesian genes (for heifers) as well calving-to-conception intervals, mean daily...

Abstract Szatkowska, I., Dybus, A., Grzesiak, W., Jedrzejczak, M. and Muszyńska, 2009. Association between the growth hormone releasing (GHRH) gene polymorphism milk production traits of dairy cattle. J. Appl. Anim. Res., 36: 119–123. The association GHRH/HaeIII polymorphism, Polish Holstein Jersey cows were analysed. A total 427 animals included in study. PCR-RFLP method was used for genotyping. frequencies genotypes alleles 0.078—AA, 0.339—AB, 0.583—BB 0.248—GHRHA , 0.752—GHRHB. For cows,...

Biotechnological processes involving the presence of microorganisms are realized by using various types stirred tanks or laboratory-scale dual-impeller commercial bioreactor. Hydrodynamics and mass transfer rate crucial parameters describing functionality efficiency bioreactors. Both strictly depend on mixing applied during bioprocesses conducted in Establishing optimum hydrodynamics conditions for process with maximizes yield desired products. Therefore, our main objective was to analyze...

The aim of this study was to compare the expression levels SOX9 (SRY-box 9) gene in testes 18 White improved male goats (Capra hircus) divided into three age groups (one, 10 and 15 months with seven, eight individuals per group, respectively). Abnormalities testis development were observed from group 10-month-olds. Additionally, differences that may affect process maturation testicular spermatogenic activity investigated among individuals. tissues analysed using qPCR. Maximal 10-month-old...

Abstract Multiwalled carbon nanotubes (MWCNTs) have attracted huge attention due to their multifunctionality. Their unique properties allows for covalent and noncovalent modifications. The most simple method functionalization of is decoration with the oxygen containing moieties which can be further simultaneously functionalized design new class carriers targeting imaging. Here, we present methodology chopping nanotubes, characterization MWCNTs, effect size on biocompatibility in culture L929...

Abstract. The relationship between the SNP of cytochrome P450 gene (CYP19-PvuII) and milk production traits Black-and-White Jersey cattle were analysed. A total 437 cows included in study. PCR-RFLP was used to genotype. frequencies genotypes alleles for as follows: 0.8985 – AA, 0.0977 AB, 0.0038 BB, 0.9474 CYP19A, 0.0526 CYP19B. In Jersey, all genotyped CYP19AA (no polymorphism). There weren’t any associations CYP19-PvuII polymorphism investigated cows.