A5100770152- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Genetics, Aging, and Longevity in Model Organisms
- Wildlife Ecology and Conservation
- MicroRNA in disease regulation
- Bacterial Genetics and Biotechnology
- Protist diversity and phylogeny
- RNA Research and Splicing
- Animal Ecology and Behavior Studies
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Microbial Natural Products and Biosynthesis
- Marine animal studies overview
- Reproductive Health and Contraception
- Pluripotent Stem Cells Research
- IgG4-Related and Inflammatory Diseases
- Fungal Biology and Applications
- Endometrial and Cervical Cancer Treatments
- Selenium in Biological Systems
- Escherichia coli research studies
- Maternal and Perinatal Health Interventions
- Statistical Methods in Clinical Trials
- Peptidase Inhibition and Analysis
- Synthesis of Organic Compounds
- Biochemical and Structural Characterization
Fujian Medical University
2023-2024
University of Tennessee at Knoxville
2024
Anhui Agricultural University
2023
Agency for Science, Technology and Research
2018-2022
Genome Institute of Singapore
2016-2020
Stanford University
2010-2015
Fanjingshan National Nature Reserve
2006-2012
Ninghai First Hospital Medicare and Health Group
2008
Three strains of Gram-negative, aerobic, neutrophilic and halophilic bacteria were isolated from samples a salt lake on the Qinghai–Tibet Plateau subterranean saline well in Si-Chuan Basin China. These isolates, designated AJ275 T , AJ282 ZG16 investigated using polyphasic approach. Based 16S rRNA gene sequence analysis, isolates could be affiliated to genus Halomonas . Genomic DNA G+C contents 65.9 mol% for 56.7 57.6 The results DNA–DNA hybridizations, fatty acid analysis physiological...
Abstract RNAs are well-suited to act as cellular sensors that detect and respond metabolite changes in the environment, due their ability fold into complex structures. Here, we introduce a genome-wide strategy called PARCEL experimentally identifies RNA aptamers vitro, high-throughput manner. By applying collection of prokaryotic eukaryotic organisms, have revealed 58 new three key metabolites, greatly expanding list natural aptamers. The newly identified exhibit significant sequence...
We have used a combination of three high-throughput RNA capture and sequencing methods to refine augment the transcriptome map well-studied genetic model, Caenorhabditis elegans . The include standard (non-directional) library preparation protocol relying on cDNA priming foldback that has been in several previous studies for characterization this species, two directional protocols, one involving direct single-stranded fragments circular-template PCR (CircLigase). find each RNA-seq approach...
The founding heterochronic microRNAs, lin-4 and let-7 , together with their validated targets well-characterized phenotypes in C. elegans offer an opportunity to test functionality of microRNAs a developmental context. In this study, we defined sequence requirements at the microRNA level for these two evaluating mutant ability support temporal development under conditions where wild-type gene products are absent. For found strong requirement seed sequences, function drastically affected by...
A Gram-negative, aerobic, neutrophilic and extremely halophilic archaeon (strain AJ201(T)), isolated from Ayakekum salt lake on the Qinghai-Tibet Plateau, was investigated by a polyphasic approach. The DNA G+C content of strain AJ201(T) 65.7 mol%. major polar lipid profile phylogenetic analysis based 16S rRNA gene sequences supported allocation to genus Halorubrum. results DNA-DNA hybridizations physiological biochemical tests allowed genotypic phenotypic differentiation closely related...
Females in most populations of chiru or Tibetan antelope Pantholops hodgsonii migrate each year up to 350 km summer calving grounds, and these migrations characterize the Tibet/Qinghai Plateau. We studied migratory population at Ullughusu grounds south-west Arjinshan Nature Reserve Xinjiang, China. The 750–1,000 km2 suitable habitat is 4,500–5,000 m with sparse vegetation. used direct methods (block counts, vehicle walking transects radial point sampling) an indirect method (pellet counts)...
In the fight against antimicrobial resistance, bacteriophages are a promising alternative to antibiotics. However, due their narrow spectra, phage therapy requires careful matching between host and bacteriophage be effective. Despite our best efforts, nature remains as only source of novel specificity. Directed evolution can potentially open an avenue for engineering specificity improving qualities phages that not strongly selected in natural environments but important therapeutic...
When designing cluster-randomized trials (CRTs), one important consideration is determining the proper sample sizes across levels and treatment conditions to cost-efficiently achieve adequate statistical power. This usually addressed in an optimal design framework by leveraging cost structures of sampling optimizing ratios hierarchy. Traditionally, optimization done through first-order derivative approach setting derivatives equal zero solve for parameters. However, method incapable properly...
Abstract Females in most populations of chiru or Tibetan antelope Pantholops hodgsonii migrate up to 350 km each year summer calving grounds. These migrations characterize the Tibet–Qinghai Plateau. The Ullughusu grounds adjacent Arjinshan Nature Reserve Xinjiang, China, are used by multiple and this ground recovered from major poaching events 1998 1999, with a population increase 2001 2006. We direct methods (vehicle/walking transects radial point sampling) an indirect method (faecal pellet...
Phomafungin is a recently reported broad spectrum antifungal compound but its biosynthetic pathway unknown. We combed publicly available Phoma genomes failed to find any putative gene cluster that could account for biosynthesis.Therefore, we sequenced the genome of one our strains (F3723) previously identified as having activity in high-throughput screen. found was predicted synthesize cyclic lipodepsipeptide differs amino acid composition compared Phomafungin. Antifungal guided isolation...
Primary endometrial squamous cell carcinoma (PESCC) is a rare malignant tumor. To investigate the clinical and pathological features of PESCC, two cases PESCC in Fujian Maternal Child Health Hospital were retrospectively studied literatures reviewed. Both menopausal women aged 57–62 years, clinically presenting with “vaginal discharge”. Case 1 was non-keratinising high-risk HPV infection. Tumor infiltrated deep myometrium multifocal intravascular thrombus macro metastases to one pelvic lymph...
The discovery of microRNAs (miRNAs) lin-4 and let-7 as temporal regulators in Caenorhabditis elegans led to broader searches for novel miRNAs their biological roles. Unlike protein-coding genes some long noncoding RNAs, canonical metazoan are not known contain introns within genomic precursor sequences. Because the short length complicates a statistically definitive assignment split RNA sequencing data sets, we took an experimental approach toward testing compatibility splicing functional...
Type 1 pili (T1P) are major virulence factors for uropathogenic Escherichia coli (UPEC), which cause both acute and recurrent urinary tract infections. T1P expression therefore is of direct relevance disease. phase variable (both piliated nonpiliated bacteria exist in a clonal population) controlled by an invertible DNA switch (fimS), contains the promoter fim operon encoding T1P. Inversion fimS stochastic but may be biased environmental conditions other signals that ultimately converge at...
Abstract Notonesomycin A is a 32-membered bioactive glycosylated macrolactone known to be produced by Streptomyces aminophilus subsp. notonesogenes 647-AV1 and S. DSM 40186. In high throughput antifungal screening campaign, we identified an alternative notonesomycin producing strain, sp. A793, its biosynthetic gene cluster. From this further characterized new more potent non-sulfated analogue, named B. Through CRISPR–Cas9 engineering of the cluster, were able increase production yield B up...
Abstract Koslov's pika, Ochotona koslowi (Büchner), was surveyed in western China. This endangered species is poorly known. We determined that it a burrowing and lives social family groups at high densities. It primarily occupies high‐elevation cold grassland vegetation. appears to have reproductive rate. More emphasis should be placed on research into this unique
MicroRNAs (miRNAs) are small, noncoding RNAs that play a crucial role in the complex and dynamic network regulates apoptosis of porcine ovarian granulosa cells (POGCs). Resveratrol (RSV) is nonflavonoid polyphenol compound involved follicular development ovulation. In previous study, we established model RSV treatment POGCs, confirming regulatory effect POGCs. To investigate miRNA-level effects on POGCs to reveal differentially expressed miRNAs, control group (n = 3, 0 μM group), low 50 high...