Surgical drains are catheters placed near the surgical wound to remove blood, pus, or other fluid and prevent it from accumulating in body. These considered major determinant for development of biofilm forming bacteria. Biofilm is an assemblage microbial cells that irreversibly associated with a surface usually enclosed matrix polysaccharide material. The aim this study investigate identify formation bacteria antibiotic resistance profile isolated drainage tube after operative. One hundred...

Urinary tract infections have become a public health concern in recent years due to antibiotic resistance. The purpose of this study is assess the antibacterial activity green synthesize gold nanoparticles on MDR bacteria. One hundred urine samples were collected from patients with urinary infections, identification and susceptibility tests bacterial isolates achieved by using Vitek 2 compact system. AuNPs was synthesized extract pomegranate peel, then detected visually changing color,...

Leuconostoc mesenteriodes is an important probiotic bacteria with impact characteristics and benefits for human health. Last year, were used as alternative to traditional antibiotics in treat of different diseases especially the broad distribution MDR bacteria. L. isolate was purchased from (Al-Amin Center Research Biotechnology\ Najaf, Iraq) re-diagnosis using MRS media anaerobic condition then diagnosis by vitek 2 system. The antibacterial activity assessed agar well diffusion method...

Abstract This study aimed to compare the effect of zein edible film containing silver nanoparticles produced by green tea leaf extract (Z‐gAgNPs) with synthetic (Z‐AgNPs) on shelf life turkey breast during refrigerated storage. The were analyzed using dynamic light scattering (DLS), UV–Vis spectroscopy, X‐ray diffraction (XRD), scanning electron microscope (SEM), and atomic force (AFM). According obtained results, fabricated (gAgNPs) showed higher polydispersity index (PDI), stability,...

This study aimed to evaluate new Staphylococcus aureus β-lactamase inhibitors from medicinal plants Rosmarinus officinalis, Ocimum basilicum, Eucalyptus globulus, and Thymus vulgaris by molecular docking analysis. The three-dimensional (3D) structures of phytochemical compounds were obtained the PubChem database. 3D structure was procured Protein Data Bank. In silico absorption, distribution, metabolism, excretion, toxicity (ADMET) analysis performed using SwissADME online server drug...

Silver nanoparticles have been considered as powerful antimicrobial agents recently, especially with the increasing incidence of diseases associated biofilm and multi-drug resistant pathogens.The aim this study was to synthesize silver by biological chemical methods combination imipenem eradicate biofilm-forming bacteria at phenotypic genotypic levels.The biosynthesis done using Enterobacter cloacae (cell-free suspension) while chemosynthesis conducted sodium borohydride.Biological were...

Date palm silver nanoparticles are a green synthesis method used as antibacterial agents. Today, there is considerable interest in it because safe, nontoxic, low costly and ecofriendly. Biofilm bacteria existing marketed local milk at highly risk on population health may be life-threatening most biofilm-forming multidrug resistance. The goal of current study to eradicate by alternative treatment nanoparticles. biofilm formation bacterial isolates was detected Congo red method. were prepared...

This study aimed to evaluate the association of intimin (eae) and pagC genes with biofilm formation multidrug resistance (MDR) phenotype in Escherichia coli Salmonella enterica collected from calves diarrhea.Fecal samples (n: 150) were diarrhea. Of 150 fecal samples, 122 (81.3%) culture positive 115/122 (94.2%) Gram-negative bacteria. Among them, E. (n = 64/115, 55.6%) was most common isolate followed by S. 41/115, 35.6%). Also, 10 (8.6%) isolates other Enterobacteriaceae bacteria including...

Serratia fonticola and Pantoea sp. are gram negative bacteria belonging Enterobactericeae, which were considered opportunistic pathogens resulted in a great number of cases nosocomial infections with serious problems multi-drug resistance, leading to increasing morbidity mortality rate.Recently, they recorded as biofilm producers.There only few studies about the capability these forming biofilm.So our aim was evaluate occurrence sp.biofilm former phenotypically genetically determination...

In Nigeria, several investigations have been done about the prevalence of AmpC enzyme in clinical isolates Gram-negative bacteria; however, little information is available on occurrence rate this important abattoir specimens that play a major role environmental pollution Nigeria. This study aimed to evaluate presence FOX AmpC-producing Pseudomonas aeruginosa from samples by both phenotypic method and polymerase chain reaction (PCR). study, 360 were analyzed for isolation P. strains....

Abstract Background Today, despite the existence of various chemical and physical treatments for wound healing, use traditional medicine including herbal is still widely used in most developed developing countries. Objectives To investigate antimicrobial wound‐healing activities alcoholic extract Boswellia carterii (BC) plant. Methods The BC was prepared using alcohol 70%. groups compounds were determined Fourier transform infrared spectroscopy (FTIR) high‐performance liquid chromatography...

Staphylococcus lentus (S. lentus) is a coagulase negative gram positive cocci recognized as opportunistic pathogens and rarely forming biofilm; it has many virulence factors, but recently caused nosocomial community infections.Biofilm formation of may be associated with the ability to resist antibiotics which leads increase in mortality rate due difficulty eradicate infections.To evaluate biofilm capacity its susceptibility antibiotics, phenotypic genotypic assays were used.Among 28...

Staphylococcuslentus is a coagulase negative gram positive cocci recognized as opportunistic pathogens and rarely biofilm forming have many virulence factors,but recently causes nosocomial community infections. Biofilm formation S. lentus may be associated with ability to resistant antibiotics lead increase in mortality rate due difficult eradicate infections To evaluate the capacity S.lentus its susceptibility by using phenotypic genotypic assay. Twenty eight bacteria among of them were...

Pseudomonas oryzihabitans is uncommon pathogen, but recently there may be warning of this bacterium to dangerous, highly virulence, and cause increase in morbidity mortality rate, especially if are multi-drug resistance biofilm former.Biofilm forming bacteria display antibiotics, reaching 1000 time higher than planktonic bacteria.The aim the current study was detect as producer determine susceptibility tested antibiotics.Pseudomonas isolated from urine catheterized patients Iraqi hospitals...

Since the potential link between virulence genes and bacterial antibiotic resistance is of great importance in medical aspects, properties such a were thoroughly investigated this work. Sixty isolates collected from patients with urinary tract infection (UTI) who visited lab at College Science/the University Al-Kufa. Preservation sub-culture these performed according to guidelines microorganism's care Research Committee Al-Qasim Green University. Several phenotypic tests involving hemolysis,...

The aim of this study was to investigate the efficacy ithmid kohl/zinc-oxide nanoparticles (ZnONPs), kohl/Aloe vera, and ZnONPs/Aloe vera in treatment bacterial endophthalmitis. endophthalmitis model prepared by contaminating both eyes 24 healthy adult male albino rabbits with a clinical isolate Klebsiella pneumoniae. animals were randomly divided into eight groups (A-H) according treatment. Group A received 1 ml kohl/ZnONPs ointment, group B gel C D, E, F treated kohl solution (0.5 g/ml...

Biofilm bacteria pose a profound threat to human and animal health. This study helps explore the potential anti-gene expression effect of green copper nanoparticles (CuONPs) on biofilm genes in strong forming E. coli The anti- gene synthesis CuONPs different concentrations (MIC Sub MIC) were evaluated against (luxS, mqsR, fliA) with housekeeping GapA by Real- time quantitive polymerase chain reaction (RT- qPCR) after treatment comparative control. results revealed that significant effective...

Pseudomonas aeruginosa (P.aeruginosa) is the most important, resistant and dangerous organism in burn wound infections human.Extracellular virulence factors lead to tissue damage during infection with P. aeruginosa.One important factor including exotoxin A(ETA) encoded by tox A gene.In this study, 45 swabs from burned skin patients admitted Al-Hilla Teaching Hospital/Babylon, 2013 different localities Babylon were collected study frequency of human immune response.Polymerase chain traction...

Silver nanoparticles have been considered a powerful antimicrobial agents recently especially after increasing incidence of diseases associated with biofilm and multi-drug resistant pathogens required to find novel path eradicate that challenge.The present study aims evaluate the antibacterial activity biosynthesized silver (AgNPs) using cell-free extract Enterobacter cloacae chemo synthesis by sodium borohydride (NaBH 4 ) on biofilm-forming Pseudomonas oryzihabitans.Antimicrobial effect in...

Multidrug-resistant bacteria (MDR bacteria) cause a significant rise in mortality, morbidity, and treatment costs. To combat the problem of MDR bacterium emergence, scientists are turning to nanoparticles general green zinc oxide Nano particle (ZnO-NPs) particular. Hence, UV spectroscopy, Fourier-Transform Infrared (FTIR) Spectroscopy, Scanning Electron Microscope (SEM), Energy Dispersive Spectroscopy (EDS) were used evaluate ZnO-NPs synthesized by synthesis technique using pomegranate...

Background: Klebsiella pneumoniae is an opportunistic pathogen usually responsible for healthcare-associated infections.Multilocus sequence typing (MLST) analysis utilized four housekeeping genes among pneumonia isolated from diarrheal humans and poultry to determine the extent of genetic diversity.Material methods: Seventy-five fecal samples both human sources were cultured diagnosed with bacterial isolates by VITEK-2 system as well Antibiotic sensitivity test (AST).Results: susceptibility...

Neisseria gonorrhoeae is a member of the Neisseriaceae family. They are non-spore-forming, gram-negative, encapsulated, non-motile, non-acidic, and bean-shaped bacteria. This study included 40 men that presented chronic recurrent infection with N. virus. The initial this virus was detected using rapid bacterial antigen immunoassay confirmed enzyme-linked immunosorbent assay (ELISA ). mean age group 36.71±12 (mean±) years. Men between 20-49 years were most affected by infection, displayed...