A5053401047- Poxvirus research and outbreaks
- HIV Research and Treatment
- Protein purification and stability
- Protein Structure and Dynamics
- Spectroscopy and Quantum Chemical Studies
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Blood properties and coagulation
- Bacterial Identification and Susceptibility Testing
- Hepatitis C virus research
- Vector-borne infectious diseases
- interferon and immune responses
- Bone health and treatments
- Antibiotic Resistance in Bacteria
- Advanced Proteomics Techniques and Applications
- Malaria Research and Control
- Parathyroid Disorders and Treatments
- Magnesium in Health and Disease
- Infections and bacterial resistance
- Molecular Biology Techniques and Applications
- Chemical Synthesis and Characterization
- Dermatological and COVID-19 studies
- Bacteriophages and microbial interactions
National Institute of Allergy and Infectious Diseases
2013-2024
National Institutes of Health
2013-2024
National Institute of Mental Health
2019
University of New Orleans
2004-2005
Southern University at New Orleans
2004-2005
Loyola University New Orleans
2003
Magnesium transporter 1 (MAGT1) critically mediates magnesium homeostasis in eukaryotes and is highly-conserved across different evolutionary branches. In humans, loss–of–function mutations the MAGT1 gene cause X-linked deficiency with Epstein-Barr virus (EBV) infection neoplasia (XMEN), a disease that has broad range of clinical immunological consequences. We have previously shown EBV susceptibility XMEN associated defective expression antiviral natural-killer group 2 member D (NKG2D)...
Abstract The alternative sigma factor RpoS plays a central role in the critical host-adaptive response of Lyme disease spirochete, Borrelia burgdorferi . We previously identified bbd18 as negative regulator but could not inactivate wild-type spirochetes. In current study we employed an inducible gene to demonstrate essential nature BBD18 for viability spirochetes vitro and at unique point vivo. Transcriptomic analyses BBD18-depleted cells demonstrated global induction RpoS-dependent genes...
Free energy calculations for the transfer of a water molecule from pure liquid to an interior cavity site in protein are presented. Two different cavities, bovine pancreatic trypsin inhibitor (BPTI) and I76A mutant barnase, represent very environments molecule: one which is polar, forming four water−protein hydrogen bonds, more hydrophobic, only bond. The give free energies with polar BPTI predicted be hydrated. corresponding entropies cavities calculated as well show that significantly...
A functional human IgA response to malaria targets a conserved epitope in the N terminus of Plasmodium falciparum circumsporozoite protein.
Abstract A new method for performing molecular dynamics simulations with fluctuating charge polarizable potentials is introduced. In models, polarizability treated by allowing the partial charges to be variables, values that are coupled on same molecule as well those other molecules. The can efficiently propagated in a simulation using extended Lagrangian dynamics. By making coordinate change from variables set of normal mode coordinates each molecule, constructed which uncouple molecule....
Carbapenem-resistant Klebsiella pneumoniae strains have emerged as a cause of life-threatening infections in susceptible individuals (e.g., transplant recipients and critically ill patients). Strains classified multilocus sequence type (ST) 258 are among the most prominent causes carbapenem-resistant K. worldwide, but basis for success this lineage remains incompletely determined. To gain more comprehensive view molecules potentially involved ST258, we used proteomics approach to identify...
Poxviruses replicate within the cytoplasm and encode proteins for DNA mRNA synthesis. To investigate poxvirus replication transcription from a new perspective, we incorporated 5-ethynyl-2'-deoxyuridine (EdU) into nascent in cells infected with vaccinia virus (VACV). The EdU-labeled was conjugated to fluor- or biotin-azide visualized by confocal, superresolution, transmission electron microscopy. Nuclear labeling decreased dramatically after infection, accompanied intense of cytoplasmic foci....
IFITs are interferon-induced proteins that can bind 5′-triphosphate or ribose-unmethylated capped ends of mRNA to inhibit translation. Although some viruses avoid by synthesizing RNAs with eukaryotic-like caps, no viral were known antagonize IFITs. We show the N- and C-terminal portions C9, a protein required for vaccinia virus resist human type I state, ubiquitin regulatory complexes, respectively. Together, two C9 domains target proteasomal degradation, thereby providing interferon...
Abstract Cellular lysates from PPD+ donors have been reported to transfer tuberculin reactivity naïve recipients, but not diphtheria reactivity, and vice versa. A historically controversial topic, the terms “transfer factor” “DLE” were used characterize reactivity-transferring properties of lysates. Intrigued by these phenomena, we found that cellular extract derived antigen-specific memory CD8+ T cells induces IL-6 antigen-matched APCs. This ultimately elicits IL-17 bystander cells. We...
The A19 protein of vaccinia virus (VACV) is conserved among chordopoxviruses, expressed late in infection, packaged the core, and required for a step morphogenesis. Multiple-sequence alignments homologs indicated conservation series lysines arginines, which could represent nuclear localization or nucleic acid binding motif, pair CXXC motifs that suggested zinc finger redox active sites. importance motif was confirmed by cysteine-to-serine substitutions, rendered altered unable to...
BACKGROUND: Vascular leakage is a deadly complication of severe infections, ranging from bacterial sepsis to malaria. Worldwide, septicemia among the top 10 causes lethality because shock and multiorgan dysfunction that arise host vascular response. In monoclonal gammopathy–associated capillary leak syndrome (MG-CLS), even otherwise mundane infections induce recurrent septic-like episodes profound microvascular hyperpermeability shock. There are no defined genetic risk factors for MG-CLS or...
Expression microdissection (xMD) is a high-throughput, operator-independent technology that enables the procurement of specific cell populations from tissue specimens. In this method, histological sections are first stained for cellular markers via either chemical or immuno-guided methods, placed in close contact with an ethylene vinyl acetate (EVA) film, and exposed to light source. The focal, transient heating cells subcellular structures melts EVA film selectively targets procurement....
Hepatitis C virus (HCV) is a member of the
Abstract Severe COVID-associated lung injury is a major confounding factor of hospitalizations and death with no effective treatments. Here, we describe non-classical fibrin clotting mechanism mediated by SARS-CoV-2 infected primary but not other susceptible epithelial cells. This infection-induced formation observed in all variants infections, requires thrombin independent tissue classical plasma coagulation factors. While prothrombin fibrinogen levels are elevated acute COVID BALF samples,...