A5053623432- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Microbial Community Ecology and Physiology
- Microbial Metabolic Engineering and Bioproduction
- PARP inhibition in cancer therapy
- Ion channel regulation and function
- Enzyme Catalysis and Immobilization
- Plant-Microbe Interactions and Immunity
- Algal biology and biofuel production
- Aquaculture Nutrition and Growth
- Microbial Fuel Cells and Bioremediation
- Protein Hydrolysis and Bioactive Peptides
- biodegradable polymer synthesis and properties
- DNA Repair Mechanisms
- Mitochondrial Function and Pathology
- Microplastics and Plastic Pollution
- Essential Oils and Antimicrobial Activity
- Metabolism and Genetic Disorders
- Microbial Metabolism and Applications
- Phytochemicals and Antioxidant Activities
- Electrochemical sensors and biosensors
- Protein Kinase Regulation and GTPase Signaling
- Tryptophan and brain disorders
- Genomics and Phylogenetic Studies
- Plant tissue culture and regeneration
Manouba University
2018-2025
Institut Supérieur de Biotechnologie de Sfax
2017-2024
Inserm
1988-1990
National Institutes of Health
1989-1990
Centre National pour la Recherche Scientifique et Technique (CNRST)
1989-1990
Centre National de la Recherche Scientifique
1985-1990
Eunice Kennedy Shriver National Institute of Child Health and Human Development
1989
In the rat liver homogenate, maximal protein kinase C activity was found at two calcium concentrations (1.75 and 3.5 mM). Subcellular fractionation of homogenate revealed that requiring 1.75 mM present only in cytosolic particulate subcellular fractions. The concentration mainly located nuclei preparation. About 19% nuclei. Goat anti-rat brain antibodies a single immunoreactive band 80-82 kDa nuclear, particulate, or Based on ratio plasma membrane marker enzyme determined nuclear...
Rat liver nuclei protein kinase C is identified as type II isozyme employing monospecific antibodies obtained against each three types of rat brain isozymes. (Yoshida, Y., Huang, F. L., Nakabayashi, H., and K-P. (1988) J. Biol. Chem. 263, 9868-9873). A major immunoreactive band at 80 kDa was revealed by step purification, nuclear extract included. The has been purified to apparent homogeneity silver nitrate staining on sodium dodecyl sulfate-polyacrylamide gel electrophoresis showing a...
Allometric growth and ontogeny were studied in thick-lipped grey mullet Chelon labrosus reared mesocosms from 1 to 71 day post hatching (dph). Multivariate allometric analysis of morphometric distinguished three distinct developmental stanzas separated by two metamorphosis lengths (Lm1 = 4.46 ± 0.06 mm; Lm2 28.56 1.04 mm). Body mass also showed episodes inflections, correlated with morpho-functional changes. First episode concerned pre-flexion larvae ended around 4.5 mm-LT (14-dph),...
The present investigation reports the isolation, molecular identification and structure elucidation of antibacterial produced by two thermophilic spore-forming bacteria from hot spring (98 °C) Guelma (Algeria). Morphological, biochemical physiological characteristics were carried out. 16S rRNA 16-23S ITS-PCR sequencing identified strains as Bacillus licheniformis with 99% similarity GenBank accession numbers KX100031 KX100032. Phenotypic characterization has mentioned several differences...
Milk and dairy products harbor a wide variety of bacterial species that compete for both limited resources space. Under these competitive conditions, bacteria develop specialized mechanisms to protect themselves during niche colonization nutrient acquisition processes. The antagonism include the production antimicrobial agents or molecules facilitate competitor dispersal. In present work, strain designated RC6 was isolated from Ricotta identified as Bacillus cereus. It generates peptide...
Mitochondrial dysfunction and oxidative stress are involved in neurodegenerative diseases associated with an enhancement of lipid peroxidation products such as 7β-hydroxycholesterol (7β-OHC). It is, therefore, important to study the ability 7β-OHC trigger mitochondrial defects, stress, metabolic dysfunctions cell death, which hallmarks neurodegeneration, identify cytoprotective molecules. The effects biotin were evaluated on 158N murine oligodendrocytes, myelin synthesizing cells, exposed...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTADP-ribosyl transferase and NAD glycohydrolase activities in rat liver mitochondriaAhmed Masmoudi Paul MandelCite this: Biochemistry 1987, 26, 7, 1965–1969Publication Date (Print):April 1987Publication History Published online1 May 2002Published inissue 7 April 1987https://pubs.acs.org/doi/10.1021/bi00381a027https://doi.org/10.1021/bi00381a027research-articleACS PublicationsRequest reuse permissionsArticle Views90Altmetric-Citations51LEARN ABOUT...
Microbial polyhydroxyalkanoates (PHA) are biodegradable and biocompatible bio-based polyesters, which used in various applications including packaging, medical coating materials. In this study, an extremophilic hydrocarbonoclastic bacterium, previously isolated from saline sediment the Tunisian desert, has been investigated for PHA production. The accumulation of intracellular granules Halomonas desertis G11 was detected by Nile blue A staining colonies. To achieve maximum yield strain G11,...
The polyketide, 6-pentyl-alpha-pyrone (6-PP), has a characteristic coconut aroma and is produced by many Trichoderma species. In this study, we screened formerly isolated strains for 6-PP production grown using solid-state fermentation (SSF). Sugarcane bagasse supplemented with nutrient solution was used as the support material culture. We detected outstandingly strong in case of two experiment. were identified based on internal transcribed spacer (ITS) sequence analysis asperellum (G7 G18)....
Abstract In recent years, the production of biopesticides has gained great attention in scientific word because it is an important alternative to replace much debated chemical pesticides used on field crops. Fungal lytic enzymes, conidia and secondary metabolites like 6 pentyl-alpha-pyrone (6-PP) play a very role biological control pests. On present study, influence application air through solid-state fermentation using three Trichoderma asperellum strains produce conidia, 6-PP essential...
The ability of Plant Growth Promoting Rhizobacteria (PGPR) to produce ammonia (NH3) is one the key mechanisms for growth and development plants. Ammonia spectrophotometric quantification assay using Nessler’s reagent remains laborious seems be not suitable large-scale PGP screening assay. Here, we performed a new accurate, easy, cost-effective micro-plate protocol in bacterial supernatants as an alternative method. In order validate accuracy our innovative microplate assay, 9 strains were...
The aim of the present work was development a new biological method for treatment textile industry effluents, which is cheaper, more profitable, and eco-friendly. This essentially based on synthesis dye-fixing peptides. use peptides synthesized via solid-phase to fix reference dye like “Cibacron blue” (CB) performance analysis binding assays were main objectives this study. For reason, two P1 (NH 2 -C-G-G-W-R-S-Q-N-Q-G-NH ) P2 -C-G-G-R-R-Y-Q-P-D-S-NH with CB by peptide (SPPS) technique....
To evaluate the efficacy of marine macro-algae <em>Chaetomorpha linum</em> as a potential biofuel resource, effects enzymatic treatment conditions on sugar yield were evaluated using three factor level Box-Behnken design. The hydrothermally pretreated <em>C. biomass was treated with <em>Aspergillus niger</em> cellulase at various liquid to solid ratios (50–100 mL/g), enzyme concentrations (10–60 U/g) and incubations times (4–44 h). Data obtained from response surface methodology subjected...
Abstract Highly purified synaptic and nonsynaptic mitochondria were prepared from rat brain, their ADP‐ribosyl transferase NAD glycohydrolase activities investigated. Data show that there is no significant difference in activity between these two types of subcellular preparations. However, appeared to be much higher mitochondria. The specific both enzymes was investigated the presence inhibitor nicotinamide or its analogue 3‐aminobenzamide other adenine nucleotides, such as ATP ADP‐ribose....
Bioremediation offers a viable alternative for the reduction of contaminants from environment, particularly petroleum and its recalcitrant derivatives. In this study, ability strain Pseudomonas BUN14 to degrade crude oil, pristane dioxin compounds, produce biosurfactants, was investigated. is halotolerant isolated polluted sediment recovered refinery harbor on Bizerte coast, north Tunisia capable producing surfactants. The assembled into 22 contigs 4,898,053 bp with mean GC content 62.4%....