A5050398954- Metabolomics and Mass Spectrometry Studies
- Liver Disease Diagnosis and Treatment
- Phytochemicals and Antioxidant Activities
- Phytoestrogen effects and research
- Mycotoxins in Agriculture and Food
- Cardiovascular Disease and Adiposity
- Vitamin C and Antioxidants Research
- Mercury impact and mitigation studies
- Endometriosis Research and Treatment
- Adipokines, Inflammation, and Metabolic Diseases
- Selenium in Biological Systems
- Marine Sponges and Natural Products
- Nuts composition and effects
- Chemotherapy-induced cardiotoxicity and mitigation
- Metabolism, Diabetes, and Cancer
- Analytical chemistry methods development
- Radiomics and Machine Learning in Medical Imaging
- Free Radicals and Antioxidants
- Adipose Tissue and Metabolism
- Immune cells in cancer
- Autophagy in Disease and Therapy
- Bioactive Compounds and Antitumor Agents
- Vitamin K Research Studies
- Blood Coagulation and Thrombosis Mechanisms
- Paraoxonase enzyme and polymorphisms
Istituto Nazionale Tumori IRCCS "Fondazione G. Pascale"
2014-2019
Istituto di Farmacologia Traslazionale
2017
Istituti di Ricovero e Cura a Carattere Scientifico
2017
Naples Anesthesia & Physician Associates
2017
Hepatocellular carcinoma (HCC) is the most common type of liver cancer and still one fatal cancers. Hence, it needs to identify always new putative markers improve its diagnosis prognosis. The selenium an essential trace mineral implicated as a key factor in early stage exerts biological function through selenoproteins. In last years our group has been studying involvement some selenoproteins HCC. However, no many data are reported literature about correlation between HCC glutathione...
In this study, a hydroalcoholic chestnut shell extract was characterized and tested on six different human cell lines. Gallic, ellagic, syringic acids were the most abundant non-condensed compounds in extract, as determined by high performance liquid chromatography (HPLC). Tannins mainly represented condensed monomeric units of epigallocatechin catechin/epicatechin. After 48 h treatment, only hepatoblastoma HepG2 cells reached an inhibition corresponding to IC50 with increase apoptosis...
Diseases are complex systems that can be studied through the integration of data derived from different disciplines to obtain a global and reliable picture biological phenomenon under investigation. Based on recent observations metabolomics profiling follicular fluids reflects ovarian microenvironment women endometriosis represents an example diseases, clearly diagnosed by laparoscopy, we thought patients represent study model evaluate possibility integrating obtained approaches. Hence, aim...
In recent years the use of natural dietary antioxidants to minimize cytotoxicity and damage induced in normal tissues by antitumor agents is gaining consideration. literature, it reported that vitamin C exhibits some degree antineoplastic activity whereas Mitoxantrone (MTZ) a synthetic anti-cancer drug with significant clinical effectiveness treatment human malignancies but severe side effects. Therefore, we have investigated effect alone or combined MTZ on MDA-MB231 MCF7 breast cancer cell...
Hepatocellular carcinoma (HCC) is among the most aggressive and fatal cancers. Its treatment with conventional chemotherapeutic agents inefficient, due to several side effects linked impaired organ function typical of liver diseases. Consequently, there exists a decisive requirement explore possible alternative chemopreventive therapeutic strategies. The use dietary antioxidants micronutrients has been proposed for HCC successful management. aim this work was test in vitro lipoic acid,...
Both type 2 diabetes (T2D) and chronic hepatitis C (CHC) infection are associated with increased risk of developing hepatocellular carcinoma (HCC). Cytokines known to play an important role not only in the mechanisms insulin resistance glucose disposal defects but also pathological processes occurring liver during viral infection. We evaluated serum levels many cytokines, chemokines, adipokines growth factors patients diabetes, CHC, CHC-related cirrhosis, CHC cirrhosis by BioPlex assay. The...
Intramuscular triglycerides (IMTGs) represent an important energy supply and a dynamic fat-storage depot that can expand during periods of elevated lipid availability fatty acid source. Ultrasonography (US) human skeletal muscles is practical reproducible method to assess both IMTG presence entity. Although crosstalk between cytokines in muscle adipose tissue has been suggested obesity, condition leading hepatic steatosis (HS) or better defined as nonalcoholic liver disease cancer, there are...
Marine sponges are an excellent source of bioactive secondary metabolites for pharmacological applications. In the present study, we evaluated chemistry, cytotoxicity and metabolomics organic extract from Mediterranean marine sponge Geodia cydonium, collected in coastal waters Gulf Naples. We identified active fraction able to block proliferation breast cancer cell lines MCF-7, MDA-MB231, MDA-MB468 induce cellular apoptosis, whereas it was inactive on normal cells (MCF-10A). Metabolomic...
Many studies have evidenced that the phenolic components from flaxseed (FS) oil potential health benefits. The effect of extract FS has been evaluated on two human breast cancer cell lines, MCF7 and MDA-MB231, non-cancerous line, MCF10A, by SRB assay, cellular death, cycle, signaling, lipid peroxidation expression some key genes. We shows anti-proliferative activity cells inducing apoptosis, increase percentage in G0/G1 phase peroxidation, activation H2AX signaling pathway, upregulation a...
Hepatocellular carcinoma is the fifth most common cancer worldwide and shows a complex clinical course, poor response to pharmacological treatment, severe prognosis. Thus, aim of this study was investigate whether tacrolimus (FK506) has synergistic antitumor effects with doxorubicin on two human hepatocellular cell lines, Huh7 HepG2. Cell viability analyzed by Sulforhodamine B assay synergic effect evaluated software CalcuSyn. apoptosis using Annexin V Dead assay. Apoptosis-related protein...
Breast cancer is one of the most frequently diagnosed forms and different treatments are used to block its progression. However, it still represents a very common cause death in women. Doxorubicin (Dox) reported as an effective agent breast treatment nonetheless induces many side‑effects. For this reason, laboratories engaged understanding how possible decrease drug concentration, considering that solutions use synergy, combining with natural substances. Recently we showed phenolic extract...
Hepatoblastoma incidence has been associated with different environmental factors even if no data are reported about a correlation between aflatoxin exposure and hepatoblastoma initiation. Considering that develops in infants children M1 (AFM1), the B1 (AFB1) hydroxylated metabolite, can be present mothers' milk marketed products, this study we decided to test effects of AFM1 on cell line (HepG2). Firstly, evaluated viability, apoptosis, cycle, metabolomic cytokinomic profile HepG2 cells...
SUMMARY Background Intramuscular triglycerides (IMTGs) represent an important energy supply and a dynamic fat-storage depot that can expand during periods of elevated lipid availability fatty ac-id source. Ultrasonography (US) human skeletal muscles is practical reproducible method to assess both IMTG presence entity. Although crosstalk between cytokines in muscle adipose tissue has been suggested obesity, condition leading hepatic steatosis (HS) or better defined as nonalcoholic liver...