A5088595910- Reproductive System and Pregnancy
- Reproductive tract infections research
- Lipid metabolism and biosynthesis
- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- Cellular transport and secretion
- Galectins and Cancer Biology
- Cervical Cancer and HPV Research
- Eicosanoids and Hypertension Pharmacology
- Diet, Metabolism, and Disease
- Cardiovascular Disease and Adiposity
- Diet and metabolism studies
- Genital Health and Disease
- Toxin Mechanisms and Immunotoxins
- Erythrocyte Function and Pathophysiology
- Glycosylation and Glycoproteins Research
- Cancer, Lipids, and Metabolism
- Lipid Membrane Structure and Behavior
- Liver Disease Diagnosis and Treatment
- Metabolism and Genetic Disorders
- Eosinophilic Esophagitis
- Reproductive Physiology in Livestock
- Adolescent Sexual and Reproductive Health
- Pancreatic function and diabetes
Centre for Genomic Regulation
2022-2025
Barcelona Institute for Science and Technology
2022-2025
Instituto de Medicina y Biología Experimental de Cuyo
2012-2023
Consejo Nacional de Investigaciones Científicas y Técnicas
2012-2023
Universidad Nacional de Cuyo
2012-2023
Instituto de Histología y Embriología de Mendoza
2018
Loss of TANGO2 in humans precipitates metabolic crises during periods heightened energy demand, such as fasting, infections, or high fever. has been implicated various functions, including lipid metabolism and heme transport, its cellular localization remains uncertain. In our study, we demonstrate that localizes to the mitochondrial lumen via a structural region containing LIL residues. Mutations these residues cause relocate periphery droplets. We further show purified binds acyl-coenzyme...
We show that TANGO2 in mammalian cells localizes predominantly to mitochondria and partially at sites juxtaposed lipid droplets (LDs) the endoplasmic reticulum. HepG2 fibroblasts of patients lacking exhibit enlarged LDs. Quantitative lipidomics revealed a marked increase lysophosphatidic acid (LPA) concomitant decrease its biosynthetic precursor phosphatidic (PA). These changes were exacerbated nutrient-starved cells. Based on our data, we suggest function is linked acyl-CoA metabolism,...
Significance Chlamydia trachomatis ( Ct ) is the most common bacterium responsible for sexually transmitted infections. It constitutes a major public health burden, with greatest clinical impact occurring in women of reproductive age. A vast proportion infections are underestimated because they asymptomatic nature. This leads to chronic severe consequences, such as ectopic pregnancy, tubal obstruction, infertility, and blindness. an obligate intracellular pathogen that completes its entire...
Abstract Agonist-mediated stimulated pathway of mucin and insulin release are biphasic in which rapid fusion pre-docked granules is followed by slow docking from the reserve pool. Here, based on a cell-culture system, we show that plasma membrane-located tetraspanin-8 sequesters syntaxin-2 to control release. Tetraspanin-8 affects during second phase The tetraspanin-8/syntaxin-2 complex does not contain VAMP-8, functions with mediate granule fusion. We suggest sequestering syntaxin-2,...
High fructose intake induces an insulin resistance state associated with metabolic syndrome (MS). The effect of vascular inflammation in this model is not completely addressed. aim study was to evaluate remodeling, inflammatory and oxidative stress markers, atheroma development high-fructose diet-induced ApoE-deficient mice (ApoE-KO). Mice were fed either a normal chow or 10% w/v (HF) drinking water over period 8 weeks. Thereafter, plasma parameters, lesion size, NAD(P)H oxidase activity the...
Rab GTPases define the identity and destiny of vesicles. Some these small present isoforms that are expressed differentially along developmental stages or in a tissue-specific manner, hence comparative analysis is difficult to achieve. Here, we describe intracellular distribution function lipid transport poorly characterized Rab39 using typical cell biology experimental tools new ones developed our laboratory. We show that, despite their amino acid sequence similarity, Rab39a Rab39b display...
Chlamydia trachomatis is the most common sexually transmitted bacterial pathogen in humans and a frequent cause of asymptomatic, persistent infections leading to serious complications, particularly young women. displays unique obligate intracellular lifestyle involving infectious elementary body replicative reticulate body. In presence stressors such as gamma-interferon (IFNγ) or beta-lactam antibiotics, C. undergoes an interruption its replication cycle enters viable but non-cultivable...
Abstract The control of the worldwide spread sexually transmitted Chlamydia trachomatis ( Ct ) infection urgently demands development a preventive vaccine. In this work, we designed vaccine based on fragment polymorphic protein D (FPmpD) that proved to be immunogenic enough generate robust systemic and mucosal IgG humoral immune response in two strains mice. We used heterologous prime-boost strategy, including simultaneous administration routes. high titers anti-PmpD antibodies elicited by...
The significant impact of Chlamydia trachomatis(Ct) infections worldwide highlights the need to develop a prophylactic vaccine that elicits effective immunity and protects host from immunopathological effects Ct infection. aim this study was evaluate based on fragment Polymorphic membrane protein D (FPmpD) C. trachomatis as an immunogen using heterologous DNA prime-protein boost strategy in female mice Three different formulations were evaluated boost: free recombinant FPmpD (rFPmpD) or...
We show that TANGO2, which lacks a transmembrane domain localizes predominantly to mitochondria and transiently endoplasmic reticulum (ER) lipid droplets (LDs). Evaluation of lipids in HepG2 cells lacking TANGO2 revealed an increase the size reactive oxygen species production. There is also marked lysophosphatidic acid (LPA) concomitant decrease its biosynthetic precursor phosphatidic (PA). These changes are exacerbated nutrient starved cells. Based on our data, we suggest principle function...