Multivesicular bodies (MVBs) are membranous structures within 60–100 nm diameter vesicles accumulate. MVBs generated after invagination and pinching off of the endosomal membrane in lumen vacuole. In certain cell types, fusion with plasma results release internal called exosomes. this report we have examined how an increase cytosolic calcium affects development exosome K562 cells overexpressing GFP‐Rab11 wt or its mutants. Rab11Q70 L mutant Rab11 wt, concentration induced by monensin caused...

Chlamydia trachomatis are obligate intracellular bacteria that survive and replicate in a bacterial-modified phagosome called inclusion. As other parasites, these subvert the phagocytic pathway to avoid degradation phagolysosomes exploit trafficking pathways acquire both energy nutrients essential for their survival. Rabs host proteins control vesicular trafficking. Rab14, Golgi-related Rab, controls Golgi endosomes transport. Since establish close relationship with apparatus, recruitment...

Significance Chlamydia trachomatis ( Ct ) is the most common bacterium responsible for sexually transmitted infections. It constitutes a major public health burden, with greatest clinical impact occurring in women of reproductive age. A vast proportion infections are underestimated because they asymptomatic nature. This leads to chronic severe consequences, such as ectopic pregnancy, tubal obstruction, infertility, and blindness. an obligate intracellular pathogen that completes its entire...

The Rab coupling protein (RCP) is a recently identified novel that belongs to the Rab11‐FIP family. RCP interacts specifically with Rab4 and Rab11, small guanosine‐5′‐triphosphatases function as regulators along endosomal recycling pathway. We used fluorescence confocal microscopy biochemical approaches evaluate participation of during particle uptake phagosome maturation. In macrophages, predominantly membrane‐bound displays punctuate vesicular pattern throughout cytoplasm. mainly...

This protocol describes the batch purification of recombinant His-tagged proteins expressed in E. coli. Proteins containing a 6xHis tag are purified using Ni-NTA (Nickel-Nitrilotriacetic Acid) beads. The His-tag allows selective binding to nickel ions, enabling efficient under native conditions. After cell lysis, lysate is incubated with beads, followed by washing and elution imidazole.

This protocol describes the batch purification of recombinant His-tagged proteins expressed in E. coli. Proteins containing a 6xHis tag are purified using Ni-NTA (Nickel-Nitrilotriacetic Acid) beads. The His-tag allows selective binding to nickel ions, enabling efficient under native conditions. After cell lysis, lysate is incubated with beads, followed by washing and elution imidazole.

Rab11 is a small GTPase that controls diverse intracellular trafficking pathways. However, the molecular machinery regulates participation of in those different transport events poorly understood. In resting cells, localizes at endocytic recycling compartment (ERC), whereas protein kinase C (PKC) isoforms display cytosolic distribution.Sustained phorbol ester stimulation induces translocation classical PKCα and PKCβII isoenzymes to ERC enriched Rab11, results transferrin inhibition....

Chlamydia trachomatis, an obligate intracellular pathogen, survives within host cells in a special compartment named 'inclusion' and takes advantage of vesicular transport pathways for its growth replication. Rab GTPases are key regulatory proteins trafficking. Several Rabs, among them Rab11 Rab14, implicated chlamydial development. FIP2, member the Rab11-Family Interacting Proteins, presents at C-terminus Rab-binding domain that interacts with both Rab14. In this study, we determined...

Rab GTPases define the identity and destiny of vesicles. Some these small present isoforms that are expressed differentially along developmental stages or in a tissue-specific manner, hence comparative analysis is difficult to achieve. Here, we describe intracellular distribution function lipid transport poorly characterized Rab39 using typical cell biology experimental tools new ones developed our laboratory. We show that, despite their amino acid sequence similarity, Rab39a Rab39b display...

During cross-presentation, exogenous antigens (i.e. intracellular pathogens or tumor cells) are internalized and processed within the endocytic system also by proteasome in cytosol. Then, antigenic peptides associated with Major Histocompatibility Complex (MHC) class I molecules these complexes transit to plasma membrane order trigger cytotoxic immune responses through activation of CD8+ T lymphocytes. Dendritic cells (DCs) particularly adapted achieve efficient antigen cross-presentation...

Chlamydia trachomatis is the causing agent of most frequent bacterial sexually-transmitted diseases worldwide and an underlying cause chronic pelvic inflammatory cervical cancer. It obligate intracellular bacterium that establishes a close relationship with Golgi complex parasites biosynthetic machinery host cells. In recent study, we have demonstrated Rab14, newly-described Golgi-associated Rab, involved in delivery sphingolipids to growing bacteria-containing vacuole. The interference...

Chlamydia trachomatis, an obligate intracellular bacterium, intercepts different trafficking pathways of the host cell to acquire essential lipids for its survival and replication, particularly from Golgi apparatus via a Rab14-mediated transport. Molecular mechanisms underlying how these bacteria manipulate transport are matter intense study. Here, we show that C. trachomatis utilizes Akt/AS160 signaling pathway promote sphingolipids delivery chlamydial inclusion through Rab14-controlled...

Chlamydia trachomatis is the causing agent of most frequent bacterial sexually-transmitted diseases worldwide and an underlying cause chronic pelvic inflammatory cervical cancer. It obligate intracellular bacterium that establishes a close relationship with Golgi complex parasites biosynthetic machinery host cells. In recent study, we have demonstrated Rab14, newly-described Golgi-associated Rab, involved in delivery sphingolipids to growing bacteria-containing vacuole. The interference...

Chlamydia trachomatis is the most common sexually transmitted bacterial pathogen in humans and a frequent cause of asymptomatic, persistent infections leading to serious complications, particularly young women. displays unique obligate intracellular lifestyle involving infectious elementary body replicative reticulate body. In presence stressors such as gamma-interferon (IFNγ) or beta-lactam antibiotics, C. undergoes an interruption its replication cycle enters viable but non-cultivable...

Pseudomonasaeruginosa uses choline as a source of carbon and nitrogen, also for the synthesis glycine betaine, an osmoprotectant under stress conditions such drought salinity. The transcription factor GbdR is specific regulator metabolism it belongs to Arac/XylS family transcriptional regulators. Despite link between catabolism bacterial pathogenicity, gbdR regulation has not been explored in detail. In present work, we describe how can be initiated from σ54-dependent promoter. activated by...

Abstract The control of the worldwide spread sexually transmitted Chlamydia trachomatis ( Ct ) infection urgently demands development a preventive vaccine. In this work, we designed vaccine based on fragment polymorphic protein D (FPmpD) that proved to be immunogenic enough generate robust systemic and mucosal IgG humoral immune response in two strains mice. We used heterologous prime-boost strategy, including simultaneous administration routes. high titers anti-PmpD antibodies elicited by...

Objective The hypertensive state is often associated with metabolic abnormalities, including glucose intolerance. Tissue kallikrein, a potent kinin-generating enzyme, present in the vascular wall and heart tissue. High dietary fructose consumption reported to induce hyperinsulinemia, hypertriglyceridemia hypertension. objective of study was examine status kallikrein cardiac tissue from highly fructose-fed rats delineate effect kinins angiotensin converting enzyme inhibitor ramipril this...