A5100738375- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Congenital heart defects research
- Electrospun Nanofibers in Biomedical Applications
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Muscle Physiology and Disorders
- Cytomegalovirus and herpesvirus research
- Neuroscience and Neural Engineering
- Colorectal Cancer Surgical Treatments
- Graphene and Nanomaterials Applications
- Platelet Disorders and Treatments
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- Surface Roughness and Optical Measurements
- Heparin-Induced Thrombocytopenia and Thrombosis
- Cardiac electrophysiology and arrhythmias
- Traumatic Brain Injury and Neurovascular Disturbances
- Dental Implant Techniques and Outcomes
- Cardiovascular Issues in Pregnancy
- MicroRNA in disease regulation
- Maternal Mental Health During Pregnancy and Postpartum
- Electrostatic Discharge in Electronics
- Nanopore and Nanochannel Transport Studies
- Corrosion Behavior and Inhibition
- Pancreatic function and diabetes
China Medical University
2024
Liaoning Provincial People's Hospital
2024
Huashan Hospital
2022
Fudan University
2022
Hebei University of Technology
2022
Shanghai Jiao Tong University
2017-2021
First Affiliated Hospital of Harbin Medical University
2014-2021
Harbin Medical University
2014-2021
Renji Hospital
2021
Nanjing Medical University
2019
Familial hypertrophic cardiomyopathy (HCM) is one the most common heart disorders, with gene mutations in cardiac sarcomere. Studying HCM patient-specific induced pluripotent stem-cell (iPSC)-derived cardiomyocytes (CMs) would benefit understanding of mechanism, as well development personalized therapeutic strategies.To investigate molecular mechanism underlying abnormal CM functions HCM, we derived iPSCs from an patient a single missense mutation (Arginine442Glycine) MYH7 gene. CMs were...
Duchenne muscular dystrophy (DMD) is caused by mutations in the dystrophin gene (DMD), and characterized progressive weakness skeletal cardiac muscles. Currently, dilated cardiomyopathy due to muscle loss one of major causes lethality late-stage DMD patients. To study molecular mechanisms underlying heart, we generated cardiomyocytes (CMs) from healthy control induced pluripotent stem cells (iPSCs). iPSC-derived CMs (iPSC-CMs) displayed deficiency, as well elevated levels resting Ca(2+),...
Rationale: Atrial fibrillation (AF) contributes significantly to morbidity and mortality in elderly hypertensive patients has been correlated enhanced atrial fibrosis. Despite a lack of direct evidence that fibrosis causes AF, reversal is considered plausible therapy. Objective: To evaluate the efficacy antifibrotic hormone relaxin (RLX) suppressing AF spontaneously rats (SHR). Methods Results: Normotensive Wistar-Kyoto (WKY) SHR were treated for 2 weeks with vehicle (WKY+V SHR+V) or RLX...
Intestinal adhesion, characterized by connection of the loops intestine with other abdominal organs fibrous tissue bands, remains an inevitable event operations and can cause a number complications. Berberine hydrochloride (berberine), natural plant alkaloid derived from Chinese herbal medicine, is diverse pharmacological effects, such as anticancer lower elevated blood glucose. This study designed to investigate effects berberine on adhesion inflammation after surgeries underlying molecular...
A variety of human inherited heart diseases affect the normal functions cardiomyocytes (CMs), endothelial cells (ECs), or smooth muscle (SMCs). To study disease and generate cardiac for basic translational research, an efficient strategy is needed production lineages from stem cells. In present study, a highly reproducible method was developed that can simultaneously enrich large number CMs SMCs ECs induced pluripotent (iPS) with high purity. Human multipotent cardiovascular progenitor were...
Human cytomegalovirus (HCMV) infection is one of the leading prenatal causes congenital mental retardation and deformities world-wide. Access to cultured human neuronal lineages, necessary understand species specific pathogenic effects HCMV, has been limited by difficulties in sustaining primary cultures. induced pluripotent stem (iPS) cells now provide an opportunity for such research. We derived iPS from adult fibroblasts neural lineages investigate their susceptibility with HCMV strain...
The reprogramming of induced cardiomyocytes (iCMs) is particular significance in regenerative medicine; however, it remains a great challenge to fabricate an efficient and safe gene delivery system induce iCMs for therapeutic applications heart injury. Here, we report branched polyethyleneimine (BP) coated nitrogen-enriched carbon dots (BP-NCDs) as highly nanocarriers loaded with microRNAs-combo (BP-NCDs/MC) cardiac reprogramming.The BP-NCDs were prepared characterized by several analytical...
Abstract Dissecting the gene expression programs which control early stage cardiovascular development is essential for understanding molecular mechanisms of human heart and disease. Here, we performed transcriptome sequencing (RNA-seq) highly purified Embryonic Stem Cells (hESCs), hESC-derived Multipotential Cardiovascular Progenitors (MCPs) MCP-specified three lineages. A novel algorithm, named as Gene Expression Pattern Analyzer (GEPA), was developed to obtain a refined lineage-specificity...
Hepatocyte transplantation is considered to be a promising therapy for patients with liver diseases. Induced pluripotent stem cells (iPSCs) provide an unlimited source the generation of functional hepatocytes. In this study, we generated iPSCs from porcine ear fibroblasts (PEFs) by overexpressing Sox2, Klf4, Oct4, and c-Myc (SKOM), developed novel strategy efficient differentiation hepatocyte-like following processes early development. The differentiated displayed phenotypes hepatocytes,...
During development, cardiac and skeletal muscle share major transcription factors sarcomere proteins which were generally regarded as specific to either or but not both in terminally differentiated adult muscle. Here, we investigated whether artificial constructed from human derived stem cells (MDSCs) recapitulates developmental similarities between We 3-dimensional collagen-based engineered tissue (EMT) using MDSCs (MDSC-EMT) compared the biochemical contractile properties with EMT induced...
Abstract This study evaluated biological compatibility and antibacterial activity of a bone substitute material consisting silver‐doped hydroxyapatite (AgHA) Bio‐Oss ® with different mixture ratios in vitro investigated its mechanism. AgHA was synthesized by chemical precipitation method. After characterization, mixed at three ratios: 1:1, 1:2, 1:4 weight. Then, Porphyromonas gingivalis (Pg) Fusobacterium nucleatum (Fn) were used to test the mixture. Human periodontal ligament fibroblasts...
Abstract Cellular cardiomyoplasty has emerged as a novel therapy to restore contractile function of injured failing myocardium. Human multipotent muscle derived stem cells (MDSC) can be potential abundant, autologous cell source for cardiac repair. However, robust conditions cardiomyocyte (CM) differentiation are not well established this type. We have developed new method CM from human MDSC that combines 3-dimensional artificial tissue (AMT) culture with temporally controlled biophysical...
Abstract Congenital human cytomegalovirus (HCMV) infection is a major cause of abnormalities and disorders in the central nervous system (CNS) and/or peripheral (PNS). However, complete pathogenesis neural differentiation caused by HCMV remains to be fully elucidated. Stem cells from exfoliated deciduous teeth (SHEDs) are mesenchymal stem (MSCs) with high proliferation neurogenic capacity. Since SHEDs originate crest early embryonic ectoderm, were hypothesized serve as promising cell line...
Heart tissue engineering holds a great potential for human heart disease therapy. Regeneration of whole biofunctional is the ultimate goal engineering. Recent advances take first step towards regeneration. However, substantial amount challenges have to be overcome.
Duchenne muscular dystrophy (DMD) is caused by mutations in the dystrophin gene (DMD), and characterized progressive weakness skeletal cardiac muscles. Currently, dilated cardiomyopathy due to muscle loss one of major causes lethality late-stage DMD patients. To study molecular mechanisms underlying heart, we generated cardiomyocytes (CMs) from healthy control induced pluripotent stem cells (iPSCs). iPSC-derived CMs (iPSC-CMs) displayed deficiency, as well elevated levels resting Ca2+,...
Clear cell renal carcinoma (ccRCC) is the most common type of malignant tumor in kidney. With numbers patients whose physical condition or stage not suitable for radical surgery, they only have a narrow choice using VEGF/mTOR targeted drugs to control their tumors, but ccRCC often shows resistance these drugs. Therefore, identifying new therapeutic target urgent necessity. In this study, first time, we concluded from bioinformatics analyses and vitro research that FK506 binding protein 10...