A5073258418- Cancer-related molecular mechanisms research
- Multiple Myeloma Research and Treatments
- RNA Research and Splicing
- Protein Degradation and Inhibitors
- RNA modifications and cancer
- PI3K/AKT/mTOR signaling in cancer
- Ubiquitin and proteasome pathways
- Histone Deacetylase Inhibitors Research
- Chronic Lymphocytic Leukemia Research
- Viral-associated cancers and disorders
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- Cancer-related Molecular Pathways
- Chemokine receptors and signaling
- Peptidase Inhibition and Analysis
- Circular RNAs in diseases
- Cancer, Hypoxia, and Metabolism
- Transplantation: Methods and Outcomes
- Chromosomal and Genetic Variations
- Lymphoma Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Cancer Mechanisms and Therapy
- Genetic factors in colorectal cancer
- MXene and MAX Phase Materials
- Growth Hormone and Insulin-like Growth Factors
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2016-2025
University of Milan
2015-2023
University of Insubria
2014-2015
The biological role and therapeutic potential of long non-coding RNAs (lncRNAs) in multiple myeloma (MM) are still to be investigated. Here, we studied the functional significance druggability oncogenic lncRNA MALAT1 MM. Targeting by novel LNA-gapmeR antisense oligonucleotide antagonized MM cell proliferation triggered apoptosis both vitro vivo a murine xenograft model human Of note, antagonism downmodulated two major transcriptional activators proteasome subunit genes, namely NRF1 NRF2,...
Although many efforts have recently contributed to improve our knowledge of molecular pathogenesis multiple myeloma (MM), the role and significance long non-coding RNAs (lncRNAs) in plasma cells (PC) malignancies remains virtually absent. To this aim, we developed a custom annotation pipeline microarray data investigating lncRNA expression PCs from 20 monoclonal gammopathies undetermined significance, 33 smoldering MM, 170 36 extra-medullary MMs/plasma cell leukemia patients, 9 healthy...
Abstract Long non-coding RNAs (lncRNAs) represent a novel class of functional RNA molecules with an important emerging role in cancer. To elucidate their potential pathogenetic chronic lymphocytic leukemia (CLL), biologically and clinically heterogeneous neoplasia, we investigated lncRNAs expression prospective series 217 early-stage Binet A CLL patients 26 different subpopulations normal B-cells, through custom annotation pipeline microarray data. Our study identified 24-lncRNA-signature...
Metastatic triple-negative breast cancer is mostly incurable, due to lack of suitable drug targets. The insulin-like growth factor (IGF) system could provide such a target, and IGF-1 receptor (IGF-1R)-directed agents are already available, but seem unable control all the complexities system, including crosstalk with hypoxia-inducible pathways.Migration MDA-231 cells its modulation by IGFs, IGF-1R inhibitor NVP-AEW541 IGF-2-sequestering monoclonal antibody MAB292 were assessed scratch wound...
// Enrica Calura 1,* , Andrea Bisognin 2,* Martina Manzoni 3 Katia Todoerti 4 Elisa Taiana Gabriele Sales 1 Gareth J. Morgan 5 Giovanni Tonon 6 Nicola Amodio 7 Pierfrancesco Tassone Antonino Neri Luca Agnelli 3,** Chiara Romualdi 1,** and Stefania Bortoluzzi 2,** Department of Biology, University Padua, Italy 2 Molecular Medicine, Clinical Sciences Community Health, Milan, Hematology Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Laboratory Pre-Clinical Translational...
Multiple myeloma (MM) is a clonal proliferation of bone marrow plasma cells characterized by highly heterogeneous genetic background and clinical course, whose pathogenesis remains largely unknown. Long ncRNAs (lncRNAs) are large class non-protein-coding RNA, involved in many physiological cellular genomic processes as well carcinogenesis tumor evolution. Although still its infancy, the role lncRNAs MM progressively expanding. Besides studies on selected candidates, expression at genome-wide...
Recent evidence indicates that protein kinase CK1α may support the growth of multiple myeloma (MM) plasma cells. Here, by analyzing a large cohort MM cases, we found high mRNA levels are virtually associated with all patients. Moreover, provided functional activity is essential for malignant cell survival even in protective niche generated co-cultures bone marrow stromal We demonstrated inactivation, while toxic cells, dispensable healthy B lymphocytes and Disruption function cells resulted...
Multiple myeloma (MM) initiation is dictated by genomic events. However, its progression from asymptomatic stages to an aggressive disease that ultimately fails respond treatments also dependent on changes of the tumor microenvironment (TME). Clonal hematopoiesis indeterminate potential (CHIP) a prevalent clonal condition hematopoietic stem cell whose presence causally linked more inflamed microenvironment. Here, we show in 106 patients with MM CHIP frequently co-existing at diagnosis,...
DIS3 gene mutations occur in roughly 10% of patients with multiple myeloma (MM); furthermore, expression can be affected by monosomy 13 and del(13q), which approximately 40% MM cases. Despite several reports on the prevalence mutations, their contribution to pathobiology remains largely unknown. We took advantage large public CoMMpass dataset investigate spectrum its impact transcriptome clinical outcome. found that relevance strictly depended co-occurrence del(13q). In particular,...
DIS3 gene mutations occur in approximately 10% of patients with multiple myeloma (MM); furthermore, expression can be affected by monosomy 13 and del(13q), found roughly 40% MM cases. Despite the high incidence deletions, biological significance its contribution to pathogenesis remains poorly understood. Herein, we investigated functional role MM, exploiting a loss function approach human cell lines. We that knockdown inhibits proliferation lines largely affects plasma cells (PC) cycle...
Multiple myeloma (MM) is a dreadful disease, marked by the uncontrolled proliferation of clonal plasma cells (PCs) within bone marrow (BM). MM characterized highly heterogeneous clinical and molecular background, supported severe genomic alterations. Important deregulation long non-coding RNAs (lncRNAs) expression has been reported in patients, influencing progression therapy resistance. NEAT1 lncRNA essential for nuclear paraspeckles involved gene regulation. We showed that supports making...
Multiple myeloma is still incurable due to an intrinsic aggressiveness or, more frequently, the interactions of malignant plasma cells with bone marrow (BM) microenvironment. Myeloma educate BM support neoplastic cell growth, survival, acquisition drug resistance resulting in disease relapse. microenvironment characterized by Notch signaling hyperactivation increased expression Notch1 and 2 ligands Jagged1 tumor cells. activation influences biology promotes reprogramming stromal In this work...
Long non-coding RNA NEAT1 is the core structural component of nuclear paraspeckle (PS) organelles and it has been found to be deregulated in multiple myeloma (MM) patients. Experimental evidence indicated that silencing negatively impacts proliferation viability MM cells, both vitro vivo, suggesting a role DNA damage repair (DDR). In order elucidate biological molecular relevance upregulation disease we exploited CRISPR/Cas9 synergistic activation mediator genome editing system engineer...