A5030688130- Cancer, Lipids, and Metabolism
- MicroRNA in disease regulation
- Endoplasmic Reticulum Stress and Disease
- Lipid metabolism and biosynthesis
- Extracellular vesicles in disease
- Prostate Cancer Treatment and Research
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Cancer-related gene regulation
- Mass Spectrometry Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Pancreatic function and diabetes
- Advanced Proteomics Techniques and Applications
- Cancer-related molecular mechanisms research
- RNA regulation and disease
- Neurogenetic and Muscular Disorders Research
- Microtubule and mitosis dynamics
- Acute Lymphoblastic Leukemia research
- Amyotrophic Lateral Sclerosis Research
- Retinal Development and Disorders
- Protein Structure and Dynamics
- Autophagy in Disease and Therapy
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- Hair Growth and Disorders
Biogen (United States)
2022
Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria
2014-2020
University of Nebraska Medical Center
2018
University of Nebraska–Lincoln
2016-2018
Sapienza University of Rome
2011-2014
Abstract Exosomes participate in cell-to-cell communication, facilitated by the transfer of RNAs, proteins and lipids from donor to recipient cells. their RNA cargos do not exclusively originate endogenous synthesis but may also be obtained dietary sources such as inter-species exosomes RNAs bovine milk humans. Here, we assessed bioavailability distribution microRNA bovine, porcine murine within across species boundaries. Milk labeled with fluorophores or fluorescent fusion accumulated...
Encapsulation of microRNAs in exosomes confers protection against degradation and a vehicle for shuttling between cells tissues, cellular uptake by endocytosis. Exosomes can be found foods including milk. Humans absorb cow's milk deliver the microRNA cargo to peripheral consistent with gene regulation dietary nucleic acids across species boundaries. Here, we tested hypothesis that human vascular endothelial transport endocytosis, constituting step crucial delivery their tissues. We this...
Hailey-Hailey disease (HHD) is an autosomal dominant disorder characterized by suprabasal cutaneous cell separation (acantholysis) leading to the development of erosive and oozing skin lesion. Micro RNAs (miRNAs) are endogenous post-transcriptional modulators gene expression with critical functions in health disease. Here, we evaluated whether specific miRNAs may play a role pathogenesis HHD. report that expressed non-random manner patients. miR-125b appeared promising candidate for playing...
Notch signaling plays a complex role in carcinogenesis, and its pathway has both tumor-suppressor oncogenic components. In this study we investigated the effects of reactive oxygen species (ROS) on Notch1 outcome keratinocyte biology. We demonstrate that function contributes to arsenic-induced transformation. found acute exposure arsenite increases oxidative stress inhibits proliferation cells by upregulation p21(waf1/Cip1). The necessity p21(waf1/Cip1) for arsenite-induced cell death was...
Single nucleotide polymorphisms (SNPs) found to be associated with Androgenetic Alopecia (AGA) date, are characterized by an apparent reduced penetrance into the phenotype suggesting a role of other factors in etiology AGA. Objective: We conducted study investigate specific allelic variants AGA controlling for nutritional and lifestyle factors. Methods: Individual patterns SNPs present baldness susceptibility locus at 20p11 (rs1160312 rs6113491) or close androgen receptor (AR) gene...
Abstract Multiple epidemiologic observations and meta-analysis clearly indicate the link between alcohol abuse incidence progression of prostate cancer; however, mechanism remains enigmatic. Recently, it was found that ethanol (EtOH) induces disorganization Golgi complex caused by impaired function largest matrix protein, giantin (GOLGB1), which, in turn, alters docking resident proteins. Here, is determined normal cells, histone deacetylase 6 (HDAC6), known regulator androgen receptor (AR)...
In hepatocytes and alcohol-metabolizing cultured cells, Golgi undergoes ethanol (EtOH)-induced disorganization. Perinuclear organized is important in liver homeostasis, but how the remains intact unknown. Work from our laboratories showed that EtOH-altered cellular function could be reversed after alcohol removal; we wanted to determine whether this recovery would apply Golgi. We used HepG2 (VA-13) cells (cultured with or without EtOH for 72 h) rat (control EtOH-fed (Lieber–DeCarli diet))....
Background MicroRNAs (miRNAs) play essential roles in gene regulation. We have reported that miRs are not only derived from endogenous synthesis but can also be obtained cow's milk. This paradigm‐shifting discovery was corroborated by our studies suggesting miRNAs, encapsulated exosomes, accumulated endocytosis human and rat intestinal cell cultures subsequently secreted across the basolateral membrane. These observations been confirmed a whole animal model. Hypotheses 1) Mice absorb...
Background Among extracellular vesicles, exosomes are of particular interest because their roles in cell‐to‐cell communication, which is facilitated by the delivery cargos such as RNAs, to recipient cells. Dietary bovine milk transported intestinal and vascular endothelial cells human rodent cell cultures, delivered peripheral tissues mice. Little known about fate following absorption. Hypothesis RNAs distinct Methods Exosomes were isolated from using ultracentrifugation. Exosome membranes...
We previously demonstrated that apoptosis induced by tocotrienols (γ and δT3) in HeLa cells is preceded Ca2+ release from the endoplasmic reticulum. This event eventually followed induction of specific calcium-dependent signals, leading to expression activation gene encoding for IRE1α protein and, turn, alternative splicing pro-apoptotic sXbp1 other molecules involved unfolded response, core pathway coping with EndoR stress. Here, we showed treatment T3s induces a set miRNAs cells. Data...
<p>3D SIM animation of the control LNCaP (c-26) cells stained with giantin (red).</p>
<p>S1. Determination of HDAC6 immunofluorescence in the nuclei PCa tissues. S2. EtOH induces Golgi fragmentation 22Rv1 cells. S3. (A) The AR W-B cytoplasmic and nuclear fractions isolated from LNCaP (c-19) S4. Colocalization GM130 GSK3β Movie S1. 3D SIM animation control (c-26) cells stained with giantin (red). after treatment (35 mM for 96 h) (red).</p>
<p>3D SIM animation of the LNCaP (c-26) cells after EtOH treatment (35 mM for 96 h) stained with giantin (red).</p>
<div>Abstract<p>Multiple epidemiologic observations and meta-analysis clearly indicate the link between alcohol abuse incidence progression of prostate cancer; however, mechanism remains enigmatic. Recently, it was found that ethanol (EtOH) induces disorganization Golgi complex caused by impaired function largest matrix protein, giantin (GOLGB1), which, in turn, alters docking resident proteins. Here, is determined normal cells, histone deacetylase 6 (HDAC6), known regulator...
<p>S1. Determination of HDAC6 immunofluorescence in the nuclei PCa tissues. S2. EtOH induces Golgi fragmentation 22Rv1 cells. S3. (A) The AR W-B cytoplasmic and nuclear fractions isolated from LNCaP (c-19) S4. Colocalization GM130 GSK3β Movie S1. 3D SIM animation control (c-26) cells stained with giantin (red). after treatment (35 mM for 96 h) (red).</p>
<p>3D SIM animation of the control LNCaP (c-26) cells stained with giantin (red).</p>
<p>3D SIM animation of the LNCaP (c-26) cells after EtOH treatment (35 mM for 96 h) stained with giantin (red).</p>
<div>Abstract<p>Multiple epidemiologic observations and meta-analysis clearly indicate the link between alcohol abuse incidence progression of prostate cancer; however, mechanism remains enigmatic. Recently, it was found that ethanol (EtOH) induces disorganization Golgi complex caused by impaired function largest matrix protein, giantin (GOLGB1), which, in turn, alters docking resident proteins. Here, is determined normal cells, histone deacetylase 6 (HDAC6), known regulator...
Abstract Golgi undergoes disorganization in response to the drugs or alcohol, but it is able restore compact structure under recovery. This self-organization mechanism remains mostly elusive, as does role of giantin, largest matrix dimeric protein. Here, we found that cells treated with Brefeldin A (BFA) ethanol (EtOH), disassembly associated giantin de-dimerization, which was restored dimer form after BFA EtOH washout. Cells lacking are disabled for restoration classical ribbon Golgi, and...