A5021852727- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Hepatitis Viruses Studies and Epidemiology
- SARS-CoV-2 and COVID-19 Research
- Liver Disease Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- Viral Infections and Vectors
- Pneumocystis jirovecii pneumonia detection and treatment
- Zoonotic diseases and public health
- Liver Disease and Transplantation
- Animal Disease Management and Epidemiology
- Infectious Diseases and Tuberculosis
- Liver Diseases and Immunity
- Immune Cell Function and Interaction
- Viral Infections and Immunology Research
- HIV-related health complications and treatments
- Mosquito-borne diseases and control
- Computational Drug Discovery Methods
- Advanced biosensing and bioanalysis techniques
- Vector-Borne Animal Diseases
- Immune Response and Inflammation
- Rabies epidemiology and control
Institut Pasteur du Cambodge
2013-2025
Stanford University
2019-2023
Inserm
2020
Délégation Paris 7
2014-2017
Université Paris Cité
2014-2017
Institut Pasteur
2006-2015
Sorbonne Paris Cité
2014
OBJECTIVE. Increasing access to highly active antiretroviral therapy reach all those in need developing countries (scale up) is slowly expanding HIV-positive children, but documented experience remains limited. We aimed describe the clinical, immunologic, and virologic outcomes of pediatric patients with >12 months 2 routine programs Cambodia. METHODS. Between June 2003 March 2005, 212 children who were younger than 13 years started therapy. Most a standard first-line regimen...
Background: To prioritize the development of antiviral compounds, it is necessary to compare their relative preclinical activity and clinical efficacy. Methods: We reviewed in vitro, animal model, studies candidate anti-coronavirus compounds placed extracted data an online relational database. Results: As August 2020, Coronavirus Antiviral Research Database (CoV-RDB; covdb.stanford.edu) contained over 2800 cell culture, entry assay, biochemical experiments, 259 model studies, 73 from 400...
We investigated the population pharmacokinetics and pharmacogenetics of efavirenz in 307 patients coinfected with human immunodeficiency virus tuberculosis included Cambodian Early vs Late Initiation Antiretrovirals trial (CAMELIA) Cambodia. Efavirenz (600 mg/d) stavudine plus lamivudine were administered addition to standard antituberculosis treatment, including rifampicin isoniazid. Blood samples obtained a mean 14 hours after intake at weeks 2 6 initiation 22 (efavirenz drugs) 50 alone)...
The frequency of transmitted HIV drug resistance (HIVDR) was evaluated in the context rapid scale-up antiretroviral treatment Thailand, Vietnam, Burkina Faso, Côte d'Ivoire, and Senegal by using an adaptation WHO generic protocol Drug Resistance Threshold Survey (HIVDR-TS) for sample collection classification. Resistance-associated mutations were interpreted 2009 list epidemiological surveys. We included 266 subjects from five study sites. Of RT PR sequences analyzed, two Vietnam harbored...
Background The number of patients on second‐line highly active antiretroviral therapy (HAART) regimens is increasing in resource‐limited settings. We describe the outcomes after 24 months for LPV/r‐based followed up by ESTHER programme Phnom Penh, Cambodia. Methods Seventy who initiated HAART more than earlier were included, and immuno‐virological data analyzed. HIV RNA viral load was determined real‐time RT‐PCR. HIV‐1 drug resistance interpreted according to ANRS algorithm. Results Of 70...
Rodent-borne viruses, including hantaviruses, arenaviruses, and rodent hepatitis virus (HEV-C), pose significant health threats to humans, causing severe diseases such as hepatitis, respiratory illness, hemorrhagic fevers. In Cambodia, data on these viruses remain limited, their burdens human are unknown. This study investigated the presences of in rodents assessed potential exposure across diverse environmental socio-economic contexts Cambodia. The was conducted urban, semi-urban, rural...
Environmental surveillance has emerged as a pivotal strategy for early detection of pathogens that pose threats to humans (1) but not been utilized zoonotic agents. In Asia, live-bird markets (LBMs) are key human-animal interfaces virus transmission (2). Traditional sampling strategies time-consuming, expensive, threaten animal welfare and have significant occupational biosafety risks. this study, we assessed the performance metagenomics on environmental samples (ES) compared traditional...
African swine fever (ASF), an economically damaging disease in domestic pigs, has emerged Vietnam since 2019. is the only country granted licenses for developing and commercializing modified live-attenuated vaccines (LAVs) against highly pathogenic ASF virus (ASFV). The nationwide implementation of LAVs prophylaxis likely influenced viral genetic pool among population. This study highlighted incursion a novel vaccine-like variant into non-vaccinated breeding herd. Retrospective epidemiology...
Bats and their ectoparasites are significant reservoirs potential vectors of emerging zoonotic pathogens, yet the viral diversity within bat-associated arthropods remains poorly characterized. This study reports identification a novel coltivirus (order Reovirales), provisionally designated Stricticimex (SCCV), in newly described bat bug species, phnomsampovensis, collected from cave-dwelling wrinkle-lipped free-tailed bats (Mops plicatus) Cambodia. Metagenomic sequencing phylogenetic...
GeneXpert® (Cepheid) is the only WHO prequalified platform for hepatitis C virus (HCV) nucleic acid amplification testing that suitable point-of-care use in resource-limited contexts. However, its application constrained by lack of evidence on genotype 6 (GT6) HCV. We evaluated field performance among a patient population Cambodia predominantly infected with GT6. Between August and September 2017, we tested plasma samples obtained from consenting patients at Médecins Sans Frontières' HCV...
To study biological factors related to protection against HIV-1 infection in Cambodia, we recruited 48 partners of HIV-1-infected patients who remained uninfected (exposed individuals, EUs) despite unprotected sexual intercourse for more than 1 year and 49 unexposed controls (UCs). HIV-1-specific antibodies (IgA anti-gp41 IgG anti-CD4-gp120 complex), T-cell responses, cellular that may be involved (peripheral blood mononuclear cell [PBMC] resistance beta-chemokine production) were evaluated....
Unconventional immune responses have been demonstrated in individuals who, despite repeated exposure to human immunodeficiency virus (HIV) infection, remain seronegative. As environmental pathogens and genetic background may modulate differentially, one Italian two Asian populations of HIV-1-exposed seronegative were studied. In serum samples from each group, IgG CCR5, CD4 IgA gp41 measured, which previously described as markers unconventional immunity HIV-exposed Caucasians. Given the...
In 2014-2015, 242 individuals aged 2-89 years were newly diagnosed with human immunodeficiency virus type 1 (HIV-1) in Roka, a rural commune Cambodia. A case-control study attributed the outbreak to unsafe injections. We aimed reconstruct likely transmission history of outbreak.We assessed 209 (86.4%) HIV-infected cases presence hepatitis C (HCV) and B (HBV). identified recent infections using antibody (Ab) avidity testing for HIV HCV. performed amplification, sequencing, evolutionary...
ART in the developing world has moved to a new era with WHO recommendation test and immediately treat HIV-positive individuals. A high frequency of pretreatment HIV drug resistance (PDR) can compromise efficacy. Our study presents updated estimates PDR seven countries from West Africa (Burkina Faso, Cameroon, Côte d'Ivoire, Mali Togo) Southeast Asia (Thailand Vietnam). Eligible participants were adult initiators, recruited December 2015 November 2016 major clinics each country. (HIVDR) tests...
BACKGROUND Recent studies conducted in developed countries described hepatitis E virus (HEV) as an emerging infectious threat to blood safety. However, data on HEV among donors from southeast Asia are lacking. STUDY DESIGN AND METHODS Between July and August 2014, we assessed the presence of immunoglobulin (Ig)G IgM 301 Cambodian donors. All samples were further tested for RNA using in‐house reverse transcription–polymerase chain reaction. ORF2/ORF3 phylogenetic analysis was performed...
Abstract In Cambodia, little epidemiological data of hepatitis C virus (HCV) is available. All previous studies were limited to only small or specific populations. the present study, we performed a characterization HCV genetic diversity based on demography, clinical data, and phylogenetic analysis non-structural 5B (NS5B) sequences belonging large cohort patients (n = 3,133) coming from majority part Cambodia between September 2016 December 2017. The revealed that genotype 1 6 most...
The paucity of hepatitis B virus (HBV) DNA measurement in low-/middle-income countries hinders the identification HBV-infected pregnant women at risk perinatal transmission. This study evaluates validity an algorithm selecting HBeAg-positive and HBeAg-negative with alanine aminotransferase (ALT) ≥40 IU/L as a predictor high HBV level.All reactive samples for surface antigen (HBsAg) were assessed SD BIOLINE HBeAg rapid test quantification was performed. Validities to identify >2 thresholds...
Background: To prioritize the development of antiviral compounds, it is necessary to compare their relative preclinical activity and clinical efficacy. Methods: We reviewed in vitro, animal model, studies candidate anti-coronavirus compounds placed extracted data an online relational database. Results: As July 2020, Coronavirus Antiviral Research Database (CoV-RDB; covdb.stanford.edu) contained >2,400 cell culture, entry assay biochemical experiments, 240 model studies, 56 from...
Abstract Between November 2006 and June 2007, HIV-1 reverse transcriptase (RT) protease (PR) genes of 67 ARV-naive Cambodian patients were amplified sequenced. At inclusion, the median age duration HIV infection 28 1.1 years, respectively. The CD4 RNA 611 cells/ml [IQR: 525-759] 4.0 log(10) copies/ml 3.4-4.6]. Among strains, 95.5% CRF 01_AE viruses (n = 64) whereas three clustered with subtype B. RT analysis indicated that only 1 patient out 67, presenting K103N M184V mutations, was...