A5059771870- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- Glutathione Transferases and Polymorphisms
- Genomics, phytochemicals, and oxidative stress
- Cancer, Hypoxia, and Metabolism
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Vitamin C and Antioxidants Research
- Redox biology and oxidative stress
- Autophagy in Disease and Therapy
- Genetics and Physical Performance
- Plant Molecular Biology Research
- Cleft Lip and Palate Research
- Drug Transport and Resistance Mechanisms
- dental development and anomalies
- Plant Genetic and Mutation Studies
- RNA Research and Splicing
- RNA modifications and cancer
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Sulfur Compounds in Biology
- Craniofacial Disorders and Treatments
- Cancer-related gene regulation
- Retinoids in leukemia and cellular processes
- Neurogenetic and Muscular Disorders Research
Los Angeles Medical Center
2012-2021
Kaiser Permanente
2012-2021
Broad Center
2013
University of Southern California
2009-2011
University of Iowa
2006-2009
Molecular Oncology (United States)
2009
University of Iowa Holden Comprehensive Cancer Center
2006-2007
University of Iowa Hospitals and Clinics
2006
Leaf senescence is the orderly dismantling of older tissue that allows recycling nutrients to developing portions plant and accompanied by major changes in gene expression. Histone modifications correlate levels expression, this study utilizes ChIP-seq classify activating H3K4me3 silencing H3K27me3 marks on a genome-wide scale for soil-grown mature naturally senescent Arabidopsis leaves. ChIPnorm was used normalize data sets identify genomic regions with significant differences two histone...
Abstract Thiol antioxidants, including N-acetyl-l-cysteine (NAC), are widely used as modulators of the intracellular redox state. We investigated hypothesis that NAC-induced reactive oxygen species (ROS) signaling perturbs cellular proliferation by regulating cell cycle regulatory protein cyclin D1 and ROS scavenging enzyme Mn–superoxide dismutase (MnSOD). When cultured in media containing NAC, mouse fibroblasts showed G1 arrest with decreased levels. The absence a overexpressing (or...
AbstractMalignant breast cancer cells often exhibit lower expression and activity of manganese superoxide dismutase (MnSOD) than their normal cell counterparts; however, the mechanism(s) responsible for this change remains unclear. We examined whether SOD2, gene encoding MnSOD, was epigenetically repressed in lines by DNA methylation histone acetylation. RT-PCR analysis SOD2 mRNA showed non-tumorigenic epithelial line MCF-10A to have two three fold higher levels either UACC-893 or MDA-MB-435...
It has been hypothesized that cancer cells increase glucose metabolism to protect against metabolic fluxes of hydroperoxides via glutathione-dependent peroxidases. 2-Deoxy-D-glucose, inhibits and shown cause cytotoxicity in is partially mediated by disruptions thiol metabolism. In the current study, human breast were continuously treated (24 hours) with 2-deoxy-D-glucose, total glutathione content as well expression first enzyme synthetic pathway [glutamate cysteine ligase (GCL)] found be...
Genome-wide association studies of colorectal cancer (CRC) have identified a number common variants associated with modest risk, including rs3802842 at chromosome 11q23.1. Several genes map to this region but does not any known transcribed or regulatory sequences. We reasoned, therefore, that is the functional single-nucleotide polymorphism (SNP), in linkage disequilibrium (LD) SNP(s). performed ChIP-seq for histone modifications SW480 and HCT-116 CRC cells, incorporated DNase I...
Transcriptional regulation of estrogen receptor-alpha (ERalpha) involves both epigenetic mechanisms and trans-active factors, such as TFAP2C, which induces ERalpha transcription through an AP-2 regulatory region in the promoter. Attempts to induce endogenous expression ERalpha-negative breast carcinomas by forced overexpression TFAP2C have not been successful. We hypothesize that chromatin structure alters activity at promoter.DNA methylation, histone acetylation, accessibility were examined...
AP-2<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>α</mml:mi></mml:math>and c-MYC are important transcription factors involved in multiple cellular processes. They each display the paradoxical capacities to stimulate both cell proliferation and apoptosis under different conditions. In present study we found that over expression of was associated with accumulation reactive oxygen species (ROS) human keratinocytes, which were significantly inhibited by co-expression AP-2. The...
Supplementary Figure 2 from Superoxide Signaling Mediates <i>N</i>-acetyl-l-cysteine–Induced G<sub>1</sub> Arrest: Regulatory Role of Cyclin D1 and Manganese Dismutase
Supplementary Figure 2 from Superoxide Signaling Mediates <i>N</i>-acetyl-l-cysteine–Induced G<sub>1</sub> Arrest: Regulatory Role of Cyclin D1 and Manganese Dismutase
Supplementary Figure 3 from Superoxide Signaling Mediates <i>N</i>-acetyl-l-cysteine–Induced G<sub>1</sub> Arrest: Regulatory Role of Cyclin D1 and Manganese Dismutase
Supplementary Figure 1 from Superoxide Signaling Mediates <i>N</i>-acetyl-l-cysteine–Induced G<sub>1</sub> Arrest: Regulatory Role of Cyclin D1 and Manganese Dismutase