Blood is considered to be a sterile microenvironment, in which bacteria appear only periodically. Previously used methods allowed for the detection of either viable with low sensitivity or selected species bacteria. The Next-Generation Sequencing method (NGS) enables identification all sample their taxonomic classification. We NGS analysis blood samples from healthy volunteers (n = 23) and patients sepsis 62) check whether any bacterial DNA exists people identify profile septic patients....

Abstract The aetiology of inflammatory bowel diseases (IBD) seems to be strongly connected changes in the enteral microbiome. dysbiosis pattern seen Crohn’s disease (CD) differs among published studies depending on patients’ age, phenotype and microbiome research methods. aims was investigate treatment-naive paediatric patients get an insight into its structure at early stage comparison healthy. Stool samples were obtained from controls newly diagnosed prior any intervention. Microbiota...

Introduction Scientific data indicate a possible influence of gut microbiota on the development type 1 and 2 diabetes mellitus (T1DM T2DM, respectively). Sequence analysis 16S ribosomal RNA identified several hundred bacterial species intestinal ecosystem, most which cannot be cultured. Objectives We aimed to evaluate composition in adult patients with T1DM T2DM establish link between microbiological test results patients' clinical data. Patients methods examined DNA isolated from fecal...

Preliminary diagnosis of fungal infections can rely on microscopic examination. However, in many cases, it does not allow unambiguous identification the species by microbiologist due to their visual similarity. Therefore, is usually necessary use additional biochemical tests. That involves costs and extends process up 10 days. Such a delay implementation targeted therapy may be grave consequence as mortality rate for immunosuppressed patients high. In this paper, we apply machine learning...

Gastrointestinal tract microbiota, particularly bacterial microflora, seem to have a different qualitative and quantitative composition in both type 1 (T1DM) 2 diabetes (T2DM) mellitus cases as compared non-diabetic individuals. So far, there are no data from research concerning the prevalence of fungi, most common genus, i.e. Candida, which important components human colon microflora. We aimed examine whether changes Candida fungi feces patients with T1DM T2DM healthy controls.Overall, we...

Numerous scientific studies confirm that, apart from environmental and genetic factors, a significant role is played by gastrointestinal microbiota in the aetiology of type 2 diabetes obesity. Currently, scientists mainly focus on distal intestinal microbiota, while equally important proximal parts intestine are overlooked. The aim study was qualitative analysis structure duodenal mucosa groups patients with obesity where qualified for bariatric surgery: sleeve gastrectomy. microbiological...

Abstract To date, publications have shown that compositions of oral microbiota differ depending on their habitats (e.g. tongue, tonsils, pharynx). The absence set standards for the choice areas and conditions material collection makes microbiome one most difficult environments a comparative analysis with other researchers, which is meaningful limitation during an assessment potential effects microorganisms as biomarkers in courses various human diseases. Therefore, standardisation basic...

Background: Hospital equipment made from copper alloys can play an important role in complementing traditional methods of disinfection. Aims the study: The aim this study was to assess dynamics antimicrobial properties selected different simulations environmental conditions (with organic contamination vs. without contamination), and test alternatives currently used testing methods. Materials Methods: A modification Japanese standard JIS Z 2801 as well Staphylococcus aureus (SA) Escherichia...

The study describes the application of PCR method for simultaneous detection DNA Gram-negative bacteria, Gram-positive yeast fungi and filamentous in blood and, thus, a whole range microbial etiological agents that may cause sepsis. Material was sterile inoculated with four species microorganisms (Escherichia coli, Staphylococcus aureus, Candida albicans Aspergillus fumigatus) collected from patients clinical symptoms developed is based on nested-multiplex real-time .Analysis obtained data...

Microbiological diagnosis of sepsis relies primarily on blood culture data. This study compares four diagnostic methods, i.e. those developed by us: nested, multiplex, qPCR (qPCR) and FISH with commercial methods: SeptiFast (Roche) (SF) BacT/ALERT® 3D system (bioMérieux). Blood samples were derived from adult patients clinical symptoms sepsis, according to SIRS criteria, hospitalized in the Intensive Care Unit.Using qPCR, FISH, SF, culture, microbial presence was found 71.8%, 29.6%, 25.3%,...

The studies on microbiome in the human digestive tract indicate that fungi could also be one of external factors affecting development diabetes. aim this study was to evaluate quantitative and qualitative mycobiome composition colon adults with type 1 (T1D), n = 26 2 (T2D) diabetes, 24 compared control group, 26. gut characterized stool samples using analysis whole internal transcribed spacer (ITS) region fungal rDNA gene cluster by next-generation sequencing (NGS) increased sensitivity. At...

The aim of the study was to determine impact biological treatment with tumor necrosis factor α antibodies (anti-TNF-α) on intestinal microbiome children severe Crohn's disease (CD) and evaluate differences in between patients treated therapy healthy children. Microbiota composition analyzed by 16S next-generation sequencing (NGS) microbial profiles were compared studied groups. Fifty-four samples (from 18 before after anti-TNF-α induction children) used analysis. Shannon's diversity index (p...

Recently, several studies explored associations between type 1 diabetes (T1DM) and microbiota. The aim of our study was to assess the colonic microbiota structure according metabolic control in T1DM patients treated with insulin pumps. We studied 89 (50.6% women) at median age 25 (IQR, 22–29) years. Pielou’s evenness (p = 0.02), Shannon’s 0.04) Simpson’s diversity indexes 0.01), were higher glycosylated hemoglobin (HbA1c) ≥ 53 mmol/mol (7%). There no differences beta groups. A linear...

Alzheimer's disease (AD) is increasingly viewed as a of synapses. Loss synapses correlates better with cognitive decline than amyloid plaques and neurofibrillary tangles, the hallmark neuropathological lesions AD. Soluble forms amyloid-β (Aβ) have emerged mediators synapse dysfunction. Aβ binds to, accumulates, aggregates in However, anatomical neurotransmitter specificity protein precursor (AβPP) AD remain poorly understood. In addition, relative roles AβPP development AD, at pre- versus...

The physiological microbiota of the vagina is responsible for providing a protective barrier, but Some factors can disturb balance in its composition. At that time, amounts genus Lactobacillus decrease, which may lead to development infection and severe complications during pregnancy. aim study was analysis bacterial composition 32 Caucasian women at each trimester pregnancy using next-generation sequencing method primers targeting V3-V4 regions. In studied group, dominant species were...

The aim of this study was to determine if there are quantitative differences in Candida fungi between pediatric patients with Crohn's disease (before and after exclusive enteral nutrition (EEN), the biologic therapy anti-tumor necrosis factor alpha - (IFX)), healthy controls. DNA isolated from fecal samples PCR used number fungal cells. Both therapeutic interventions resulted a statistically significant decrease Pediatric Disease Activity Index. numbers decreased during both intervention but...

Background . Type 2 diabetes mellitus (T2DM) is determined by genetic and environmental factors. There have been many studies on the relationship between composition of gastrointestinal bacterial flora, T2DM, obesity. are no data, however, gut microbiome structure in monogenic forms disease including Maturity Onset Diabetes Young (MODY). Methods The aim investigation was to compare qualitative parameters colonic flora patients with HNF1A - MODY T2DM healthy individuals. 16S sequencing DNA...

Abstract The sphingolipid analog fingolimod is known to induce apoptosis of tumor cells and lymphocytes. Its effect on neutrophils has not been investigated so far. Here, we describe a fingolimod-induced atypical cell death mechanism in human neutrophils, characterized by rapid translocation heat shock protein 27 the surface, extensive swelling vacuolization, chromatin staining nuclear morphology, phosphorylation mixed lineage kinase domain-like protein. Fingolimod also induces typical...

The aim of this study was to determine quantitative changes in selected species bacteria (Bacteroides fragilis, Lactobacillus fermentum, rhamnosus, Serratia marcescens) the stool patients with Crohn's disease (CD) course induction treatment exclusive enteral nutrition (EEN) or anti-tumor necrosis factor alpha (Infliximab, IFX) vs. healthy controls (HC). DNA isolated from samples CD (n = 122) and HC 17), real-time Polymerase Chain Reaction (qPCR) applied. In both groups, first sample taken...

Abstract Background Complex interactions between the brain, gut and adipose tissue allow to recognize obesity as a neurometabolic disorder. The recent data have shown that microbiota can play potential role in development. Transcranial direct current stimulation (tDCS) is safe non-invasive technique modulate activity of cerebral cortex other connected brain areas also context appetite control. objective this study was evaluate effects repetitive anodal tDCS (AtDCS) prefrontal on feeding...

Diagnostics of the coronavirus disease 2019 (COVID-19) using molecular techniques from collected respiratory swab specimens requires well-equipped laboratory and qualified personnel, also it needs several hours waiting for results is expensive. Antigen tests appear to be faster cheaper but their sensitivity specificity are debatable. The aim this study was compare a selected antigen test with quantitative polymerase chain reaction (qPCR) results. Nasopharyngeal swabs were 192 patients...

Abstract Background Bovine mastitis is a widespread disease affecting dairy cattle worldwide and it generates substantial losses for farmers. Mastitis may be caused by bacteria, fungi or algae. The most common species isolated from infected milk are, among others, Streptococcus spp. , Escherichia coli Staphylococcus aureus non-aureus staphylococci mammaliicocci. aim of this paper to determine the frequency occurrence bacterial in samples cows with three regions Poland: north-east, south-west...

The effects of SARS‑CoV‑2 infection on the composition upper respiratory tract (URT) microbiota are yet to be established, and more attention this topic is needed.The study aimed assess bacterial profile possible association between URT viral load.Nasopharyngeal swabs were taken from 60 adult patients with who divided into 3 groups based quantification cycle (Cq) value in quantitative polymerase chain reaction test: group I (n = 20), Cq lower than or equal 31 (high replication rate); II...

The aim of the study was to evaluate particular polymerase chain reaction primers targeting selected representative genes and influence a preincubation step in selective broth on sensitivity group B Streptococcus (GBS) detection by nucleic acid amplification techniques (NAAT). Research samples were vaginal rectal swabs collected duplicate from 97 pregnant women. They used for enrichment culture-based diagnostics, bacterial DNA isolation, amplification, using based species-specific 16S rRNA,...

The study aim was evaluation of the usefulness several thermostable DNA polymerases in real time PCR conducted presence heme. Our had advantage testing different polymerases, one which proved to be least sensitive heme activity. We also found that there is no need supplementing reaction mixture with protective substances like BSA. Selection appropriate polymerase can increase efficiency very important for diagnosis sepsis and other analyses performed on template isolated from blood.