A5046064167- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Veterinary medicine and infectious diseases
- Herpesvirus Infections and Treatments
- Vibrio bacteria research studies
- Microbial infections and disease research
- Escherichia coli research studies
- Plant Virus Research Studies
- Protein purification and stability
- Carbohydrate Chemistry and Synthesis
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Poxvirus research and outbreaks
- Galectins and Cancer Biology
- Photosynthetic Processes and Mechanisms
- RNA Interference and Gene Delivery
Westlake University
2023-2025
Fudan University
2023-2024
State Key Laboratory of Genetic Engineering
2024
Henan Agricultural University
2010-2011
O-GlcNAcylation is a conserved post-translational modification that attaches N-acetyl glucosamine (GlcNAc) to myriad cellular proteins. In response nutritional and hormonal signals, regulates diverse processes by modulating the stability, structure, function of target Dysregulation has been implicated in pathogenesis cancer, diabetes, neurodegeneration. A single pair enzymes, O-GlcNAc transferase (OGT) O-GlcNAcase (OGA), catalyzes addition removal on over 3,000 proteins human proteome....
Abstract Mpox virus (MPXV) can cause mpox in humans. Due to its quick and wide spread the past two years, has turned into a significant public health concern. Helicase E5 is multi-domain protein; primer synthesis DNA unwinding activity are required for genome uncoating replication of MPXV. However, vitro never been demonstrated. Here, we report structural biochemical studies MPXV E5, showing that full-length protein adopts an auto-inhibited conformation. Truncation N-terminus recover towards...
Abstract The DdmDE antiplasmid system, consisting of the helicase-nuclease DdmD and prokaryotic Argonaute (pAgo) protein DdmE, plays a crucial role in defending Vibrio cholerae against plasmids. Guided by DNA, DdmE specifically targets plasmids, disassembles dimer, forms DdmD–DdmE handover complex to facilitate plasmid degradation. However, precise ATP-dependent DNA translocation mechanism has remained unclear. Here, we present cryo-EM structures bound single-stranded (ssDNA)...
Chromatin loading of the hexameric replicative helicase MCM2-7 complex requires coordinated interactions with origin recognition (ORC), CDC6, and CDT1. not bound to DNA forms a single hexamer (SH) an open entry gate. Two SHs are loaded sequentially form double (DH) that encircles duplex. Activated then unwinds initiates replication. Our cryo-electron microscopy analyses show fraction human without exists as DH. Unexpectedly, we find MCM3 winged helix domain (WHD) docks on MCM2 in both...
Abstract The VCPIP1‐P97/VCP (Valosin‐Containing Protein) complex is required for post‐mitotic Golgi cisternae reassembly and maintenance in interphase. However, the organization mechanism of this regulating membrane fusion still elusive. Here, cryo‐electron microscopy (cryo‐EM) structures human are presented. These studies reveal that three independent VCPIP1 molecules sit over C‐terminal substrate exit tunnel formed by P97/VCP homo‐hexamer, resulting an unusual C3 to C6 symmetric barrel...