A5060375530- Enzyme Catalysis and Immobilization
- Chemical Synthesis and Analysis
- Microbial Metabolic Engineering and Bioproduction
- Advanced Electron Microscopy Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Force Microscopy Techniques and Applications
- Biochemical and Molecular Research
- Integrated Circuits and Semiconductor Failure Analysis
- Biofuel production and bioconversion
- RNA and protein synthesis mechanisms
University of Groningen
2024-2025
Genetic incorporation of noncanonical amino acids (ncAAs) harbouring catalytic side chains into proteins allows the creation enzymes able to catalyse reactions that have no equivalent in nature. Here, we...
Genetic incorporation of noncanonical amino acids (ncAAs) harboring catalytic side chains into proteins allows the creation enzymes able to catalyze reactions that have no equivalent in nature. Here, we present for first time use ncAA 3-aminotyrosine (aY) as residue a designer enzyme iminium activation catalysis. Incorporation aY protein scaffold LmrR gave rise an artificial Friedel-Crafts (FC) alkylase exhibiting complementary enantioselectivity previous FC-alkylase design using...
Genetically encoded noncanonical amino acids can introduce new-to-nature activation modes into enzymes. While these act as catalysts on their own due to inherent chemical properties, interactions with adjacent residues in an enzyme, such those present natural catalytic dyads or triads, unlock a higher potential for designer We incorporated boron-containing acid the protein scaffold RamR create active enzyme kinetic resolution of α-hydroxythioesters. found that closely positioned lysine...