A5009727876- Hematopoietic Stem Cell Transplantation
- Acute Myeloid Leukemia Research
- Genomics and Chromatin Dynamics
- Acute Lymphoblastic Leukemia research
- Hematological disorders and diagnostics
- Hepatitis C virus research
- Mesenchymal stem cell research
- Single-cell and spatial transcriptomics
- Blood disorders and treatments
- Chronic Myeloid Leukemia Treatments
- Hepatitis B Virus Studies
- Immune cells in cancer
- Immune Cell Function and Interaction
- HIV Research and Treatment
- RNA Research and Splicing
- Polyomavirus and related diseases
- Telomeres, Telomerase, and Senescence
- Childhood Cancer Survivors' Quality of Life
- T-cell and Retrovirus Studies
- Liver Disease Diagnosis and Treatment
- Renal Transplantation Outcomes and Treatments
- NF-κB Signaling Pathways
- Nuclear Structure and Function
- Liver Diseases and Immunity
- Myeloproliferative Neoplasms: Diagnosis and Treatment
Oncode Institute
2022-2024
The Netherlands Cancer Institute
2020-2024
Medizinische Hochschule Hannover
1992-2024
Max Planck Institute of Psychiatry
2023
Novartis Institutes for BioMedical Research
2021
Novartis (Switzerland)
2021
Medical University of Vienna
2015
Austrian Red Cross
2013
Austrian Cluster for Tissue Regeneration
2013
Dana-Farber Cancer Institute
1993-1996
Abstract Chromosome structure in mammals is thought to regulate transcription by modulating three-dimensional interactions between enhancers and promoters, notably through CTCF-mediated loops topologically associating domains (TADs) 1–4 . However, how chromosome are actually translated into transcriptional outputs remains unclear. Here, address this question, we use an assay position enhancer at large numbers of densely spaced chromosomal locations relative a fixed promoter, measure promoter...
There is an increased risk of GVHD and non-relapse mortality (NRM) after allogeneic stem cell transplantations (alloSCT) when mismatched unrelated donors (MMUD) are used. In Europe, it standard practice to use rabbit anti-thymocyte globulin (rATG) reduce the high NRM risks MMUD alloSCT. As alternative rATG, post-transplantation Cyclophosphamide (PTCy) in increasing clinical use. It currently impossible give general recommendations regarding preference for one method over another since...
Fetal calf serum (FCS) bears a potential risk for carrying diseases and eliciting immune reactions. Nevertheless, it still represents the gold standard as medium supplement in cell culture. In present study, human platelet lysate (PL) was tested an alternative to FCS expansion subsequent chondrogenic differentiation of adipose-derived stem cells (ASCs). ASCs were expanded with 10% (group F) or 5% PL P). Subsequently, three-dimensional (3D) micromass pellets created cultured 5 weeks medium....
Abstract Genes are often activated by enhancers located at large genomic distances. The importance of this positioning is poorly understood. By relocating promoter-reporter constructs into >1,000 alternative positions within a single locus, we dissected the positional relationship between mouse Sox2 gene and its distal enhancer. This revealed an intricate, sharply confined activation landscape, in which native occupies optimal position for activation. Deletion relaxes confinement broadly...
Combination of functional epigenomics and single-cell CRISPR screens reveals that ER exerts its oncogenic role via downstream TFs.
Abstract Lamina-associated domains (LADs) are megabase-sized genomic regions that interact with the nuclear lamina (NL). It is not yet understood how their interactions NL encoded in DNA. Here, we designed an efficient LAD “scrambling” approach, based on transposon-mediated local hopping of loxP recombination sites, to generate series large deletions and inversions span LADs flanking sequences. Mapping these rearrangements revealed a single contacts through multiple act cooperatively or...
Abstract The epigenetic environment plays an important role in DNA damage recognition and repair, both at double-strand breaks deprotected telomeres. To increase understanding on how responses (DDR) telomeres are regulated by modification remodeling of telomeric chromatin we screened 38 methyltransferases for their ability to promote telomere dysfunction-induced genomic instability. As top hit identified MMSET, a histone methyltransferase (HMT) causally linked multiple myeloma...
As a result of pathophysiologically unexplainable bone marrow failure, most patients with progressive stages human immunodeficiency virus (HIV) infection develop anemia, leukopenia, and thrombocytopenia. Besides the possibility immune-mediated cytolysis or direct viral hemopoietic progenitor cells, inhibitory influence cytokines, for example interferon-α (IFN-α) IFN-γ, on hemopoiesis HIV-infected might be considered as one parameter that contributes to myelosuppression. Therefore, cells from...
Abstract Schizophrenia (SCZ) is a highly polygenic disease and genome wide association studies have identified thousands of genetic variants that are statistically associated with this psychiatric disorder. However, our ability to translate these associations into insights on the mechanisms has been challenging since causal variants, their molecular function target genes remain largely unknown. In order address questions, we established functional genomics pipeline in combination induced...
Background: Sofosbuvir (SOF), Simeprevir (SMV) and Daclatasvir (DCV) are reimbursed for chronic hepatitis C patients with advanced liver disease (F3, F4) posttransplant in Austria.