A5109847798- Microbial infections and disease research
- Aquaculture disease management and microbiota
- Toxoplasma gondii Research Studies
- Antimicrobial Resistance in Staphylococcus
- Viral gastroenteritis research and epidemiology
- Antibiotics Pharmacokinetics and Efficacy
- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Bioactive Compounds and Antitumor Agents
- Plant and fungal interactions
- Helminth infection and control
- Nosocomial Infections in ICU
- Heme Oxygenase-1 and Carbon Monoxide
- Infective Endocarditis Diagnosis and Management
- Helicobacter pylori-related gastroenterology studies
- Chromatography in Natural Products
- Electroconvulsive Therapy Studies
- Pathogenesis and Treatment of Hiccups
- Vector-Borne Animal Diseases
- Treatment of Major Depression
- Ion channel regulation and function
- Anesthesia and Pain Management
- Listeria monocytogenes in Food Safety
- Pain Management and Opioid Use
- Academic Writing and Publishing
Central Mental Hospital
2014
Oregon State University
2009-2012
University of Nebraska–Lincoln
2012
University of Veterinary Medicine
2012
Louisiana State University
2012
Association of American Veterinary Medical Colleges
2012
Virginia–Maryland College of Veterinary Medicine
2012
Grace (United States)
2012
Royal Veterinary College
2012
University of London
2012
Campylobacter jejuni is the most common bacterial cause of diarrhea worldwide. To colonize gut and infection, C. must successfully compete with endogenous microbes for nutrients, resist host defenses, persist in intestine, ultimately infect host. These challenges require expression a battery colonization virulence determinants. In this study, intestinal lifestyle was studied using whole-genome microarray, mutagenesis, rabbit ileal loop model. Genes associated wide range metabolic,...
Ewing, P. J., Burrows, G., MacAllister, C, Clarke, C. Comparison of oral erythromycin formulations in the horse using pharmacokinetic profiles. J. vet. Pharmacol, Therap. 17 , 17–23. The properties four were compared six adult horses after administration single and multiple doses. Formulations administered estolate phosphate given 37.5 mg/kg every 12 h 25 8 h, stearate ethylsuccinate h. Areas under curve ( AUC ) maximum plasma concentrations (C max equal or greater (P ≥ 0.05) following with...
Despite the prevalence of C. jejuni as an important food borne pathogen, microbial factors governing its infection process are poorly characterized. In this study, we developed a novel rabbit soft tissue chamber model to investigate interactions with host. The in vivo transcriptome profile was monitored function time post-infection by competitive microarray hybridization cDNA obtained from grown vitro. Genome-wide expression analysis identified 449 genes expressed at significantly different...
Isolated neutrophils were used to study the intracellular calcium ([Ca2+]i) dependency of Pasteurella haemolytica leukotoxin-induced production leukotriene B4 and plasma membrane damage. Exposure leukotoxin caused a rapid concentration-dependent increase in [Ca2+]i, followed by simultaneous damage B4. Removal extracellular Ca2+, replacement Ca2+ with other divalent cations, or exposure high concentration verapamil, an inhibitor voltage-dependent channels, inhibited increases production,...
ABSTRACT Exposure of bovine neutrophils to Pasteurella haemolytica leukotoxin (LKT) stimulates the production leukotriene B 4 (LTB ), which is believed be an important chemotactic agent in development acute fibrinopurulent pneumonic infection cattle. The involvement phospholipase A 2 (PLA ) LKT-induced synthesis LTB was studied by using labeled with 3 H-arachidonate ([ H]AA). Incubation isolated [ H]AA resulted incorporation radioactivity PLA substrates phosphatidylcholine,...
Isolated bovine neutrophils were used to study the relationship between duration and magnitude of Mannheimia haemolytica leukotoxin-induced increase in intracellular calcium concentration leukotriene B4 synthesis. In contrast recombinant human C5a, which caused a transient, small no effects on synthesis, exposure leukotoxin resulted rapid, sustained, large concentration, followed by This response was similar those produced ionophore, A23187, phorbol myristate acetate, also significant...
The contribution of intracellular calcium stores to Mannheimia haemolytica leukotoxin (LKT)-induced increase in cytosolic concentration was studied by pharmacologically inhibiting transport across the plasma and endoplasmic reticulum membranes bovine neutrophils exposed LKT. Active storage sarcoplasmic/endoplasmic ATPase, influx extracellular membrane, release stored via inositol triphosphate receptors ryanodine-sensitive channels were inhibited using thapsigargin, lanthanum chloride,...
Aim To evaluate pharmacokinetic and pharmacodynamic interactions between tamsulosin acenocoumarol. Methods Twelve healthy volunteers received 0.4 mg or placebo once daily for 9 days in a double‐blind, cross‐over study. On day 5 of each study period, single 10‐mg oral dose racemic acenocoumarol was administered. Results The ratios (point estimates (90% confidence intervals)) values the presence absence were: AUC PT 1.01 (0.98, 1.03); maximum prothrombin time (Pt max ) 0.99 (0.94, 1.05);...
Abstract Rapid and sensitive detection of methicillin‐resistant Staphylococcus aureus is crucial for effective treatment control clinical infections caused by this bacterium. The goal study to develop a CE‐based method multiplexed identification femA sequence specific S. unique mecA encoding methicillin resistance. Blood samples spiked with known concentrations bacteria were used testing. Crude cell lysates prepared treating the blood DNazol Direct reagent as template isothermal...
Background Co-prescription of linezolid and serotonergic agents (SSRIs, SNRIs, NaSSA, TCAs) can lead to serotonin syndrome, this study seeks identify prescribing practise these agents. Methods Study all general hospital inpatients prescribed intravenous in a 3-month period, using drug charts clinical notes. Results occurred 20% with SSRIs most frequently concurrently used. There were no cases syndrome. was evidence notes vigilance for potential interaction. Conclusion is common; awareness...
The objective of this study was to evaluate the penetration ceftiofur- and desfuroylceftiofur-related metabolites (DCA) into sterile infected tissue chambers, lung disposition DCA in plasma across four different sacrifice days postdosing. Twelve healthy calves were utilized following implantation with chambers paralumbar fossa. Tissue each calf randomly inoculated either Mannheimia haemolytica or PBS. All dosed ceftiofur crystalline free acid suspension (CCFA-SS) subcutaneously ear pinna....
Large subcutaneous fluid swellings developed on some of the sheep in two flocks infested with Psoroptes ovis during weeks after they had been plunge-dipped phenol-based solutions. The contained between 1 and 10 litres exudate affected 4 per cent each flock. or exudative dermatitis significantly lower serum albumin concentrations than unaffected (P < 0.05). In most cases secondary bacterial infections occurred these necessitated intensive antibiotic therapy. severity skin lesions posed a...
Pasteurella haemolytica serotype A1 (bovine strain OK) was incubated for 2 and 6 h in bovine subcutaneous tissue chambers vivo, ovine 82-25 L011 were vitro heat-inactivated or serum from which gamma globulin had been depleted by protein G affinity chromatography to assess changes morphology lectin agglutination profiles (strains L101 only). Cells, removed after h, covered with an extensive, dense glycocalyx extending approximately 0.5 microm the cell surface. In many cells, separated surface...
Journal Article Comprehensibilty of papers Get access Cyril Clarke 43 Caldy Road, West Kirby, Wirral Search for other works by this author on: Oxford Academic PubMed Google Scholar QJM: An International Medicine, Volume 86, Issue 3, March 1993, Page 215, https://doi.org/10.1093/oxfordjournals.qjmed.a068793 Published: 01 1993