A5069575896- Gastric Cancer Management and Outcomes
- Metastasis and carcinoma case studies
- Cancer-related molecular mechanisms research
- Gastrointestinal Tumor Research and Treatment
- Angiogenesis and VEGF in Cancer
- MicroRNA in disease regulation
- Cell Adhesion Molecules Research
- Colorectal Cancer Surgical Treatments
- Esophageal and GI Pathology
- Cancer Research and Treatments
- Cancer-related gene regulation
- Bariatric Surgery and Outcomes
- Circular RNAs in diseases
- Peptidase Inhibition and Analysis
- Pancreatic and Hepatic Oncology Research
- Glycosylation and Glycoproteins Research
- Proteoglycans and glycosaminoglycans research
- Cancer Cells and Metastasis
- Abdominal Trauma and Injuries
- Colorectal and Anal Carcinomas
- Clinical Nutrition and Gastroenterology
- Fibroblast Growth Factor Research
- Liver Disease and Transplantation
- Neuroendocrine Tumor Research Advances
- MRI in cancer diagnosis
Zhejiang Provincial People's Hospital
2011-2024
Hangzhou Medical College
2018-2024
Zhejiang Hospital
2008-2017
Wenzhou Medical University
2008-2015
Hebei North University
2008-2013
Zhejiang University
2008
Zhejiang Chinese Medical University
2008
Zhejiang Provincial Natural Science Foundation
2008
Baylor College of Medicine
2008
The present study investigated the clinical significance of secreted protein, acidic and rich in cysteine (SPARC), development progression gastric cancer.Immunohistochemistry was used to analyze SPARC, integrin beta1, matrix metalloproteinase (MMP)-2 expression 436 clinicopathologically characterized cancer cases.SPARC, MMP-2 protein levels were upregulated lesions compared with adjacent noncancerous tissues. SPARC detected 334 human cases highly expressed 239 tumors. We also found a...
Oridonin (ORI) could inhibit the proliferation and induce apoptosis in various cancer cell lines. However, mechanism is not fully understood.Human prostate (HPC) cells were cultured vitro viability was detected by CCK-8 assay. The ultrastructure changes observed under transmission electron microscope (TEM). Chemical staining with acridine orange (AO), MDC or DAPI used to detect acidic vesicular organelles (AVOs) alternation of DNA. Expression LC3 P21 Western Blot. Apoptotic rates cycle...
Gastric cancer (GC) has been a major health burden all over the world but there are fewer promising chemotherapeutic drugs due to its multidrug resistance. It reported that WYC-209 suppresses growth and metastasis of tumor-repopulating cells effect on GC was not explored. MTT, colony formation, transwell assays were performed examine effects proliferation, growth, mobility cells. Western blotting qRT-PCR used detect expression proteins mRNA. RNA-seq enrichment analyses conducted for...
Abstract Background The metalloproteinase domain‐containing protein 10 (ADAM 10) has been implicated in the development and progression of gastric cancer. Methods Expression ADAM C‐erbB‐2 were examined immunochemically 436 clinicopathologically characterized cancer cases. Results Protein levels up‐regulated lesions compared with adjacent non‐cancerous tissues. Positive expression correlated age, size tumor, location depth invasion, vessel lymph node, distant metastasis TNM stage, also...
Abstract Background The role of annexin II in the development and progression gastric cancer was explored. Methods Real-time PCR conducted to detect S100A6 mRNA expression. Protein expressions were also examined by immunohistochemistry 436 clinicopathologically characterized cases. Results expression differ significantly among tumor tissue matched non-cancerous mucosa. levels up-regulated compared with adjacent tissues. High correlated age, location tumor, size differentiation, histological...
This study aims to investigate the effect of miR-10b overexpression on cancer cell proliferation, migration, invasion, and Hoxd10 expression.The invasion MKN-28, BGC-823, SGC-7901 cells expression protein in BGC-823 were detected following transfection inhibitor or Negative Control B. Expression 436 paraffin-embedded tissues was also investigated.miR-10b significantly upregulated AGS, HCG-27, SGC-7901, MKN-45 lines, inhibited proliferation migration MKN-45, 48 h after transfection, while...
Abundant macrophage infiltration and increased expression of coagulation factors have been observed in cancer patients. The aim the present study was to determine how interaction between activated monocytes/macrophages contributes gastric (GC) cell migration invasion. We assessed cytokine/chemokine production coagulation-factor-treated macrophages by ELISA. effects tumor-associated (TAM) on GC invasion were determined vitro assay. In addition, we used an co-culture system cells/TAM treated...
To investigate the prognostic significance of tumor necrosis factor receptor (TNFR),-associated 6 (TRAF6),-and ubiquitin in gastric cancer patients. Biopsies rectus abdominis muscle were obtained intra operatively from 102 patients and 29 subjects undergoing surgery for benign abdominal diseases, TRAF6 mRNA expression proteasome proteolytic activities assessed. was significantly upregulated compared with control muscles. 67.65% (69/102) cancer. Over muscles associated TNM stage, level serum...
L1 cell adhesion molecule (L1CAM) and epithelial (EPCAM) have been implicated in the development progression of gastric cancer. The present study investigated clinical significance L1CAM EPCAM development, prognosis cancer.Expression were examined immunochemically 601 clinicopathologically characterized cancer cases.L1CAM protein was detected 23.9% human non-tumor mucosa samples. All samples expressed at low levels. High expression 163 (27.1%) tumors. Expression correlated with age, tumor...
Background and Objectives miR-301a is significantly overexpressed in many cancers. However, its expression biological role gastric cancer remain poorly understood. We investigated microRNA-301a (miR-301a) determined effects on cell behavior clinical significance the development progression of cancer. Methods tumors lines by reverse transcription-polymerase chain reaction. The clone formation, migration, invasion HGC-27 SGC-7901 cells were detected following transfection an inhibitor. a...
Background Since the first laparoscopic splenectomy (LS) in 1991, LS has become gold standard for removal of normal-to-moderately enlarged spleens benign conditions. Compared with open (OS), fewer postsurgical complications and better postoperative recovery were observed, but it is contraindicated hypersplenism secondary to liver cirrhosis owing technical difficulties associated splenomegaly, well-developed collateral circulation, increased risk bleeding. With improvements technique, concept...
Epithelial-mesenchymal transition (EMT) is an important biological process that characteristic of malignant tumor cells with metastatic potential. We investigated the role miR-551b in EMT and metastasis gastric cancer (GC). found low levels were associated EMT, a poor prognosis GC patients. Further, two cell lines, MNK45 SGC7901, exhibited lower than GES normal stomach line. Exposing SGC7901 to TGF-β1 resulted morphology changes which was confirmed by Western blot analysis demonstrating...
Gastric cancer (GC) is the second leading cause of cancer‑associated mortality worldwide. Although rate patients with GC has improved, it remains a significant health issue. The MYC proto‑oncogene protein serves key roles in cellular proliferation, differentiation, transformation and apoptosis. Previous studies have identified abnormal expression MYC‑binding (MYCBP) during tumorigenesis multiple types cancer. Furthermore, evidence demonstrates that MYCBP contributes to invasion migration...
Downregulation of KAI1 gene expression has been found in many types cancer cells and is closely related to invasion metastasis. This study was aimed at investigating the effects possible underlying mechanisms on metastasis human hepatocellular carcinoma (HCC).The invasive ability, visco-elastic properties cell adhesion forces were analysed different HCC originating from MHCC97-H line transfected with either sense or antisense plasmid. Tumuorigenicity, metastatic abilities, extracellular...
analysis demonstrated that the mean survival time and 5-year rate were lower in cases with low expressions of syndecan-1 E-cadherin high expression integrin β3 (P < 0.01, all cases).COX multivariate showed level could be an independent prognostic index gastric carcinoma 0.01), whereas not indexes > 0.05, P 0.05 respectively). CONCLUSION:The are significantly correlated invasion metastasis carcinoma, they highly each other.Therefore may serve as important markers carcinoma.
Abstract To investigate matrix metalloproteinase‐9 (MMP‐9) mRNA and vascular endothelial growth factor (VEGF) protein expression in gastric carcinoma its correlation with microvascular density, growth‐pattern, invasion, metastasis, prognosis. In situ hybridization of MMP‐9 immunohistochemistry VEGF CD34 proteins were performed on surgical specimens cancers from 118 patients compared 20 nonmalignant mucosae. Their relationships to pathological parameters survival times determined by...