A5018525114- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Endoplasmic Reticulum Stress and Disease
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Cellular transport and secretion
- Peptidase Inhibition and Analysis
- European and International Law Studies
- Fungal and yeast genetics research
- Chromatin Remodeling and Cancer
- Signaling Pathways in Disease
- RNA and protein synthesis mechanisms
- Genetics and Neurodevelopmental Disorders
- Turkey's Politics and Society
- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Protein Degradation and Inhibitors
- Tissue Engineering and Regenerative Medicine
- Transplantation: Methods and Outcomes
- CAR-T cell therapy research
- RNA Research and Splicing
- Genetics, Aging, and Longevity in Model Organisms
University of Dundee
2012-2024
Wellcome Trust
2024
Omsk State University
2015
University of Göttingen
2012
Chromatin organization must be maintained during cell proliferation to preserve cellular identity and genome integrity. However, DNA replication results in transient displacement of DNA-bound proteins, it is unclear how they regain access newly replicated DNA. Using quantitative proteomics coupled Nascent Capture or isolation Proteins on DNA, we provide time-resolved binding kinetics for thousands proteins behind replisomes within euchromatin heterochromatin human cells. This shows that most...
The proteins from the UBA-UBX family interact with ubiquitylated via their UBA domain and p97 UBX domain, thereby acting as substrate-binding adaptors for ATPase. In particular, human UBXN7 (also known UBXD7) mediates interaction transcription factor HIF1α that is actively in normoxic cells by a CUL2-based E3 ligase, CRL2. Mass spectrometry analysis of protein immunoprecipitates showed they multitude ubiquitin-ligases. Conspicuously, was most proficient interacting cullin-RING ligase...
Abstract Understanding how chromatin organisation is duplicated on the two daughter strands a central question in epigenetics. In mammals, following passage of replisome, nucleosomes lose their defined positioning and transcription contributes to re-organisation. However, whether plays greater role organization DNA replication remains unclear. Here we analysed protein re-association with newly replicated upon inhibition using iPOND coupled quantitative mass spectrometry. We show that...
FAM111A is a replisome-associated protein and dominant mutations within its trypsin-like peptidase domain are linked to severe human developmental syndrome, the Kenny–Caffey syndrome. However, functions remain unclear. Here, we show that facilitates efficient activation of DNA replication origins. Upon hydroxyurea treatment, FAM111A-depleted cells exhibit reduced single-stranded formation better survival rate. Unrestrained expression WT patient mutants causes accumulation damage cell death,...
RACK1/Asc1p and its essential orthologues in higher eukaryotes, such as RACK1 metazoa, are involved several distinct cellular signaling processes. The implications of a total deletion have never been assessed comprehensive manner. This study reveals the major processes affected Saccharomyces cerevisiae Δasc1 background via de novo proteome transcriptome analysis, well subsequent phenotypical characterizations. ASC1 reduces iron uptake causes nitrosative stress, both known indicators for...
Summary Elongator is a conserved, multi‐protein complex discovered in S accharomyces cerevisiae , loss of which confers range pleiotropic phenotypes. higher eukaryotes required for normal growth and development mutation the largest subunit human ( Elp 1) causes familial dysautonomia, severe recessive neuropathy. promotes addition mcm 5 ncm modifications to uridine tRNA anticodon ‘wobble’ position both yeast eukaryotes. Since these are tRNAs function efficiently, translation defect caused by...
Ubiquitination regulates several biological processes, however the role of specific members ubiquitinome on intracellular membrane trafficking is not yet fully understood. Here, we search for ubiquitin-related genes implicated in protein performing a High-Content siRNA Screening including 1187 human “ubiquitinome” using amyloid precursor (APP) as reporter. We identified deubiquitinating enzyme PSMD14, subunit 19S regulatory particle proteasome, K63-Ub chains cells, novel regulator...
ABSTRACT Ubiquitination regulates several biological processes. Here, we search for ubiquitin-related genes implicated in protein membrane trafficking performing a High-Content siRNA Screening including 1,187 of the human “ubiquitinome” using Amyloid Precursor Protein (APP) as reporter. We identified deubiquitinating enzyme PSMD14, subunit 19S regulatory particle proteasome, specific K63-Ub chains cells, novel key regulator Golgi-to-endoplasmic reticulum (ER) retrograde transport. Silencing...
ABSTRACT FAM111A is a replisome associated protein and dominant mutations within its trypsin-like peptidase domain are linked to severe human developmental syndromes. However, functions putative substrates remain largely unknown. Here, we showed that promotes origin activation interacts with the FAM111B, identified first potential substrate, suicide enzyme HMCES. Moreover, unrestrained expression of wild-type patient mutants impaired DNA replication caused cell death only when remained...
Abstract DNA replication results in the transient eviction of nucleosomes, RNAPII and transcription regulators. How chromatin organization is duplicated on two daughter strands a central question epigenetics. In mammals, restarts newly replicated within couple hours, promoting accessibility. However, role restoration other determinants following remains unclear. Here we have monitored protein re-association to upon inhibition using iPOND coupled quantitative mass spectrometry. We show that...