A5108582614- Glioma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Neurological disorders and treatments
- Advanced Proteomics Techniques and Applications
- Virus-based gene therapy research
- RNA modifications and cancer
- Neuroscience and Neural Engineering
- RNA Interference and Gene Delivery
- Epigenetics and DNA Methylation
- Angiogenesis and VEGF in Cancer
- Pluripotent Stem Cells Research
- Cancer Research and Treatments
- S100 Proteins and Annexins
- Molecular Biology Techniques and Applications
- Gene expression and cancer classification
- Neurogenesis and neuroplasticity mechanisms
- Vitamin D Research Studies
- Cancer-related Molecular Pathways
- Mass Spectrometry Techniques and Applications
- Neuroethics, Human Enhancement, Biomedical Innovations
- 3D Printing in Biomedical Research
- Neuroscience and Neuropharmacology Research
- Cell Adhesion Molecules Research
- CRISPR and Genetic Engineering
Inserm
2015-2025
Centre Hospitalier Universitaire de Grenoble
2012-2025
CEA Grenoble
2012-2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2012-2025
Institut polytechnique de Grenoble
2013-2025
Brain Tech Laboratory
2018-2025
Nantes Université
2025
Center for Research in Transplantation and Translational Immunology
2025
École Centrale de Nantes
2025
CEA LETI
2008-2024
The aim of this study was to identify predictor sets genes whose over- or underexpression in human sporadic adrenocortical tumors would help malignant vs. benign and predict postsurgical metastatic recurrence. For this, we analyzed the expression 230 candidate using cDNA microarrays a series 57 well-characterized (33 adenomas 24 carcinomas). We identified two clusters (the IGF-II cluster containing eight genes, including IGF-II, steroidogenesis six encoding steroidogenic enzymes plus other...
Nicotine has many effects on CNS functions, presumably through its action neuronal nicotinic acetylcholine receptors (AChRs). One subclass of AChRs that binds the snake venom toxin alpha-bungarotoxin (alpha-Bgt-AChRs) been shown to modulate neurotransmission in brain. We now show alpha-Bgt-AChR activation by low doses nicotine results apoptotic cell death both primary and immortalized hippocampal progenitor cells. In HC2S2-immortalized progenitors, is cytotoxic undifferentiated cells,...
Recent results have shown the ability of bone marrow cells to migrate in brain and acquire neuronal or glial characteristics. In vitro, marrow-derived MSCs can be induced by chemical compounds express markers these lineages. an effort set up a mouse model such differentiation, we addressed potentiality (mMSCs) that recently purified. These expressed nestin, specific marker neural progenitors. Under differentiating conditions, mMSCs display distinct shape NF-L (neurofilament-light,...
Cytotoxicity to 5-fluoro-2'-deoxyuridine (FdUrd) derives from its conversion 5-fluorodeoxyuridine-5'-monophosphate, which binds and inhibits thymidylate synthase (TS) in the presence of cofactor, 5,10-methylenetetrahydrofolate. We have selected FdUrd-resistant variants human cell line HEp-2 following adaptation stepwise increases drug concentration. In initial selection, maximal resistance was associated with a 26-fold increase cellular level TS. Greater TS overproduction (80-fold) obtained...
Gliomas such as oligodendrogliomas (ODG) and glioblastomas (GBM) are brain tumours with different clinical outcomes. Histology-based classification of these tumour types is often difficult. Therefore the first aim this study was to gain microRNA data that can be used reliable signatures glioblastomas. We investigated levels 282 microRNAs using membrane-array hybridisation real-time PCR in ODG, GBM control tissues. In comparison tissues, 26 deregulated were identified tissue seven (miR-21,...
Objective. We investigated SF and serum proteomic fingerprints of patients suffering from RA, OA other miscellaneous inflammatory arthritides (MIAs) in order to identify RA-specific biomarkers. Methods. profiles 65 31 were studied by surface-enhanced laser desorption ionization–time-of-flight-mass spectrometry technology. The most discriminating RA biomarkers identified matrix-assisted ionization–time flight their overexpression was confirmed western blotting ELISA. Results. Three 10 839,...
Although there is now evidence that the expression of centromeric (CT) and pericentric (PCT) sequences are key players in major genomic functions, their transcriptional status human cells still poorly known. The main reason for this lack data complexity high level polymorphism these repeated sequences, which hampers straightforward analyses by available transcriptomic approaches. Here a macro-array dedicated to analysis CT PCT developed validated heat-shocked (HS) HeLa cells. For first time,...
Epidemiological and experimental studies suggest that 1,25-dihydroxyvitamin D3 (1,25D) plays a neuroprotective role in neurodegenerative diseases including Alzheimer's disease. Most of the data regarding genes regulated by this hormone brain cells have been obtained with neuron glial cells. Pericytes play critical function encompasses their classical blood-brain barrier control maintenance. However, gene response pericyte to 1,25D remains be investigated. Analyses transcriptomic human...
In this paper, we present the first proof of concept confirming possibility to record magnetoencephalographic (MEG) signals with optically pumped magnetometers (OPMs) based on parametric resonance 4He atoms. The main advantage kind OPM is provide a tri-axis vector measurement magnetic field at room-temperature (the vapor neither cooled nor heated). sensor achieves sensitivity 210 fT/ √ Hz in bandwidth [2-300 Hz]. MEG simulation studies brain phantom were cross-validated real measurements...
Gene transfer into muscle by electroporation with low-voltage and long-pulse (LV/LP, 100 V/50 msec) currents was shown to be more efficient than simple intramuscular DNA injection. Nevertheless, transgene expression declined from day 7 only reached 10% of the maximum 3 weeks after electroporation. We have optimized conditions including voltage, pulse number, amount injected luciferaseencoding plasmid in tibialis anterior muscle. Using high-voltage short-pulse (HV/SP, 900 V/100 mu sec)...
Although oral administration of L-Dopa remains the best therapy for Parkinson disease, its long-term causes appearance abnormal involuntary movements such as dyskinesia. persistent striatal induction some genes has already been associated with pathologic profiles in hemiparkinsonian rats, molecular and cellular mechanisms underlying adaptations remain to be elucidated. In this study, using a rat model L-Dopa-induced dyskinesia, we report that activity regulated cytoskeletal (Arc)-associated...
Abstract Stem cells always balance between self‐renewal and differentiation. Hence, stem cell culture parameters are critical need to be continuously refined according progress in our biology understanding the latest technological developments. In past few years, major efforts have been made define more precisely medium composition which grow or differentiate. This led progressive replacement of ill‐defined additives such as serum feeder layers by recombinant cytokines growth factors....
<h3>Objectives:</h3> The use of biologicals such as infliximab has dramatically improved the treatment rheumatoid arthritis (RA). However, factors predictive therapeutic response need to be identified. A proteomic study was performed prior therapy identify a panel candidate protein biomarkers RA response. <h3>Methods:</h3> Plasma profiles 60 patients with (28 non-responders (as defined by American College Rheumatology 20% improvement criteria (ACR20)) negative and 32 responders (ACR70...
The aim of this study was to correlate MRI features and molecular characteristics in anaplastic oligodendrogliomas (AOs). 50 AO patients enrolled the French national network for high-grade oligodendroglial tumors were analyzed. genomic profiles IDH mutational statuses assessed using high-resolution single-nucleotide polymorphism arrays direct sequencing, respectively. gene expression 25 1p/19q-codeleted AOs studied on Affymetrix arrays. Most cases frontal lobe contrast-enhanced (52%), but...
We report the first successful technology integration of chiplets on an active silicon interposer, fully processed, packaged and tested. Benefits chiplet-based architectures are discussed. Built up is presented focused 3D interconnects process characterization. packaging to structural test characterization demonstrator.
In this paper, we present a proof of concept study which demonstrates for the first time possibility recording magnetocardiography (MCG) signals with 4He vector optically pumped magnetometers (OPM) operated in gradiometer mode. Resulting from compromise between sensitivity, size and operability clinical environment, developed are based on parametric resonance helium zero magnetic field. Sensors at room temperature provide tri-axis measurement Measured sensitivity is around 210 f T (√Hz)-1...
Abstract The secosteroid 1,25‐dihydroxyvitamin D 3 (1,25 (OH)D 2 DD is the major biologically active metabolite of vitamin D. Antitumor activity this hormone has been observed on several cell lines and breast cancer in vivo. purpose vitro study was to determine possible effect 1,25(OH) glioma cells. Two from rat (C6) or human (GHD) origin were cultured presence . sensitivity these cells 1,259OH) assessed with a colorimetric MTT assay. A cytotoxic detected at concentrations around 10 −8 M....