A5002290433- Insect Resistance and Genetics
- Insect and Pesticide Research
- Insect Pest Control Strategies
- Entomopathogenic Microorganisms in Pest Control
- Genetically Modified Organisms Research
- Neuroscience and Neuropharmacology Research
- Bacillus and Francisella bacterial research
- Hypothalamic control of reproductive hormones
- Viral Infectious Diseases and Gene Expression in Insects
- Ion channel regulation and function
- Agricultural pest management studies
- Pancreatic function and diabetes
- Estrogen and related hormone effects
- Photosynthetic Processes and Mechanisms
- Neuroendocrine regulation and behavior
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Toxin Mechanisms and Immunotoxins
- Receptor Mechanisms and Signaling
- Animal Diversity and Health Studies
- Bioactive Compounds and Antitumor Agents
- Chemical Synthesis and Reactions
- Neuroscience and Neural Engineering
- Steroid Chemistry and Biochemistry
- Fungal and yeast genetics research
- Healthcare Systems and Practices
Purdue University West Lafayette
2024
Natural Sciences and Engineering Research Council
2010
Université de Montréal
1995-2010
Biotechnology Research Institute
1997-2001
National Institute of Diabetes and Digestive and Kidney Diseases
1997
National Institutes of Health
1997
University of Ottawa
1976-1994
National Research Council Canada
1977-1992
Institute for Biological Sciences
1991
University of Chicago Medical Center
1988
We have investigated the effects of steroids on intracellular calcium ion concentration [Ca2+]i in chicken granulosa cells obtained from two largest preovulatory follicles laying hens. was measured loaded with Ca(2+)-responsive fluorescent dye fura-2. The resting these 100 +/- 5 nM. There an immediate (i.e. less than sec) 4- to 8-fold increase all 76 examined after addition 10(-7) M estradiol-17 bdta. Estradiol-17 beta effective between 10(-10)-10(-6) M. alpha, estrone, and estriol...
The role of the third domain CryIAa, a Bacillus thuringiensis insecticidal toxin, in toxin-induced membrane permeabilization receptor-free environment was investigated. Planar lipid bilayer experiments were conducted with parental toxin and five proteins obtained by site-directed mutagenesis block 4, an arginine-rich, highly conserved region protein. Four mutants constructed replacing first arginine position 21 lysine (R521K), glutamine (R521Q), histidine (R521H), or glutamic acid (R521E). A...
Upstream open reading frames (uORFs) are short peptide-encoding sequences located in the 5' untranslated region (5' UTR) of mRNAs, enabling translational repression main (m)ORFs. While uORFs found ∼50% mRNAs humans, our understanding their biological function remains limited. This study aims to elucidate role uORF UTR Gata4 (GATA binding protein 4) gene cardiac biology by inactivating its start codon (ΔuORF) mouse genome. Our investigation reveals that mice with inactivation manifest...
To test whether the ability of Bacillus thuringiensis toxins to form pores in midgut epithelial cell membrane susceptible insects correlates with their vivo toxicity, we measured effects different on electrical potential apical freshly isolated midguts from gypsy moth (Lymantria dispar) and silkworm (Bombyx mori) larvae. In absence toxin, potential, a conventional glass microelectrode, was stable for up 30 min. It sensitive K+ concentration oxygenation external medium. Addition which L....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMechanism of the catalyzed thio-Claisen reaction. Triggering concerted rearrangement processesH. Kwart and J. L. SchwartzCite this: Org. Chem. 1974, 39, 11, 1575–1583Publication Date (Print):May 1, 1974Publication History Published online1 May 2002Published inissue 1 1974https://pubs.acs.org/doi/10.1021/jo00924a027https://doi.org/10.1021/jo00924a027research-articleACS PublicationsRequest reuse permissionsArticle Views363Altmetric-Citations33LEARN...
Polycyclic aromatic hydrocarbons (PAHs) are widespread environmental contaminants whose metabolism in mammals results deleterious cell transformation. Covalent modification of DNA by diol epoxides metabolically formed from PAHs such as benzo[a]pyrene (BaP) provides a mechanism for the genotoxicity, mutagenicity, and carcinogenicity PAHs. We had previously reported NMR evidence minor conformer duplex d(G1G2T3C4A5*C6G7A8G9)·d(C10T11C12G13G14G15A16C17C18) containing dG14 mismatch opposite dA5*...
The effects of the muscarinic cholinergic receptor agonist carbachol on intracellular calcium ion concentration ([Ca2+]i) and progesterone production was determined in granulosa cells from two largest preovulatory follicles laying hens. [Ca2+]i measured loaded with calcium-responsive fluorescent dye fura-2. Resting 96 +/- 5 nM (n = 13). There a 4- to 8-fold increase 85% 80) within 10 sec after addition maximally stimulating (2 mM) carbachol. initial spike followed by sustained, but lower,...
The modulation of intracellular free calcium concentration ([Ca2+]i) by cAMP was compared in pancreatic beta-cells lean (+/+) and obese (ob/ob) mice. Neither forskolin nor 8-bromo-cAMP significantly affected basal [Ca2+]i unstimulated mouse beta-cells. In obese, but not beta-cells, adding or during the glucose-induced response triggered external Ca(2+)-dependent oscillations with a duration 5-11 s frequency 2.3-4.8 min-1. induction required both stimulatory glucose membrane depolarization....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTpH, temperature, and concentration dependence of the chemical shift scalar coupling constants in disodium hydrogen phosphite fluorophosphateT. C. Farrar, J. L. Schwartz, S. RodriguezCite this: Phys. Chem. 1993, 97, 28, 7201–7207Publication Date (Print):July 1, 1993Publication History Published online1 May 2002Published inissue 1 July 1993https://pubs.acs.org/doi/10.1021/j100130a013https://doi.org/10.1021/j100130a013research-articleACS...
Glucose-induced shifts in intracellular free Ca2+ concentration ([Ca2+]i) were quantitatively and temporally the same ob/ob +/+ beta-cells. In both, epinephrine promptly protractedly inhibited glucose-induced [Ca2+]i surge via a pertussis toxin-sensitive alpha 2-adrenergic mechanism that was reversible by potassium depolarization. When added before glucose, blocked completely beta-cells, but beta-cells it produced delayed, reduced, transient (Ca2+i) surge. Neither ATP-sensitive K+ channel...
Various hormones and growth factors act at least in part by raising the cytosolic pH of target cell. In present studies we examined influence muscarinic cholinergic agonist carbachol on intracellular (pHi) chicken granulosa cells. The pHi cells from two largest preovulatory follicles hens was determined spectrofluorometrically using pH-sensitive dye 2',7'-bis-(carboxyethyl)- 5(6)-carboxyfluorescein (BCECF). Carbachol (0.1-2 mM) induced a concentration-dependent increase pHi, which reached...