A5077015059- DNA and Nucleic Acid Chemistry
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Underwater Vehicles and Communication Systems
- Underwater Acoustics Research
- Pesticide Exposure and Toxicity
- Protease and Inhibitor Mechanisms
- Immunotherapy and Immune Responses
- Toxin Mechanisms and Immunotoxins
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Vitamin K Research Studies
- Metabolomics and Mass Spectrometry Studies
- Maritime Navigation and Safety
- Silk-based biomaterials and applications
- Pesticide and Herbicide Environmental Studies
- Target Tracking and Data Fusion in Sensor Networks
- Chemical Reaction Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Cyclization and Aryne Chemistry
- Cytomegalovirus and herpesvirus research
- Transgenic Plants and Applications
- Advanced Biosensing Techniques and Applications
- Bone and Dental Protein Studies
University of Colorado Denver
2007-2023
National Jewish Health
2007-2023
Tennessee Technological University
2015-2018
National Institutes of Health
1997-2014
United States Army
2010
U.S. Army Combat Capabilities Development Command Chemical Biological Center
2010
University of Colorado Health
2006-2007
Columbia University
2006
Naval Information Warfare Center Pacific
2003-2006
United States Army Combat Capabilities Development Command
2006
Cyclophilin, a specific cytosolic binding protein responsible for the concentration of immunosuppressant cyclosporin A by lymphoid cells, was purified to homogeneity from bovine thymocytes. Cation-exchange high-performance liquid chromatography resolved major and minor cyclophilin species that bind with dissociation constant about 2 × 10 -7 moles per liter activities 77 67 micrograms milligram protein, respectively. Both have an apparent molecular weight 15,000, isoelectric point 9.6, nearly...
Worldwide, millions of people are exposed to arsenic in drinking water that exceeds the World Health Organization standard 10 μg/liter by as much 50–300-fold, yet little is known about molecular basis for excretion. Here we show transport into bile depends on MRP2/cMOAT transporter and glutathione obligatory such transport. Using reversed phase liquid chromatography/mass spectrometry, demonstrate two arsenic-glutathione complexes not previously identified <i>in vivo</i>, triglutathione...
Abstract To better understand the biochemistry of matrix-forming cells, we developed a simple and reproducible procedure for isolation identification by N-terminal sequencing proteins secreted cells into culture medium applied this to analysis major Coomassie blue-staining under 100 kD that are from three different human osteoblastic cell cultures. The normal osteoblasts adult trabecular bone were identified be gelatinase, osteonectin, C-terminal propeptides α1 α2 chains type I collagen,...
B cells have multiple roles in immune activation and inflammation separate from their capacity to produce antibodies. cell depletion is currently under intense investigation as a therapeutic strategy for autoimmune diseases. The TNF family members cell–activating factor of the (BAFF) its homolog A proliferation-inducing ligand (APRIL) are survival differentiation factors therefore rational targets. We compared effects BAFF receptor–Ig, which blocks only BAFF, with those transmembrane...
Abstract Laser‐excited Raman spectra of the double‐helical complexes poly A·poly U and G·poly C are reported for 2 H O solutions. The discussed in relation to their use as quantitative reference determining dependence scattering RNA on secondary structure. line at 815 cm −1 , due phosphodiester group, exhibits same intrinsic intensity is thus dependent only upon amount ordering helix not kinds nucleotides involved. hypochromic lines identified changes determined quantitatively over...
Prolactin is a peptide hormone produced by the anterior pituitary gland that critical in lactation. can also be lymphocytes, and both B T cells express prolactin receptors. These findings have suggested has immunomodulatory functions. Studies spontaneously autoimmune hosts demonstrated role for augmenting autoreactivity. We chose to analyze effects on anti-DNA nonspontaneously female BALB/c mice transgenic heavy chain of an antibody. Treatment with 4 weeks induced lupus-like phenotype...
Seaweb '98, '99, and 2000 begin a series of annual experiments incrementally advancing telesonar underwater acoustic signaling ranging technology for undersea wireless networks. The constraints imposed by transmission through shallow water channels have yielded channel-tolerant methods, hybrid multi-user access strategies, novel network topologies, half-duplex handshake protocols, iterative power-control techniques. Seawebs '98 '99 respectively included ten fifteen battery-powered, anchored...
Prolactin is a peptide hormone produced by the anterior pituitary gland that critical in lactation. can also be lymphocytes, and both B T cells express prolactin receptors. These findings have suggested has immunomodulatory functions. Studies spontaneously autoimmune hosts demonstrated role for augmenting autoreactivity. We chose to analyze effects on anti-DNA nonspontaneously female BALB/c mice transgenic heavy chain of an antibody. Treatment with 4 weeks induced lupus-like phenotype...
Abstract The present studies demonstrate that matrix Gla protein (MGP), a 10‐kDa vitamin K‐dependent protein, is phosphorylated at 3 serine residues near its N‐terminus. Phosphoserine was identified 3, 6, and 9 of bovine, human, rat, lamb MGP by N‐terminal sequencing. All modified serines are in tandemly repeated Ser‐X‐Glu sequences. Two the shark MGP, 2 5, also have glutamate n + position sequences, whereas third, residue would acquire an acidic phosphoserine upon phosphorylation 5....
Biotoxin activity assays typically involve multistep sample preparation, multicomponent reactions, analysis, or a combination thereof. We report single-step, real-time ricin assay that requires little no preparation and employs direct analysis in real time mass spectrometry. The release of adenine from the inhomogeneous substrate herring sperm DNA by was determined to be 53 ± 2 pmol per picomole hour. This procedure can readily adapted any enzyme for which reactant product low molecular...
Receptor editing or secondary Ig gene rearrangement occurs in immature, autoreactive B cells to maintain self-tolerance. Here we show that nonspontaneously autoimmune mice immunized with a peptide mimetope of DNA develop peptide- and DNA-reactive antibodies. Antigen-specific display follicular cell phenotype. As these move into the memory compartment, many express RAG protein acquire expression both κ λ light chains. Thus, this study provides evidence for receptor occurring mature,...
The solution structure of an 11-mer DNA duplex, d(CGGTCA*CGAGG) x d(CCTCGTGACCG), containing a 10R adduct at dA* that corresponds to the cis addition N(6)-amino group dA(6) (+)-(9S,10R)-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene was studied by 2D NMR methods. NOESY cross-peak patterns indicate hydrocarbon is intercalated on 5'-side modified base. This observation same as observed for other oligonucleotides (10R)-dA adducts but opposite corresponding (10S)-dA which are 3'-side from major...
Abstract Raman spectra of poly(rG), obtained from neutral aqueous solutions containing excess sodium counterions, are qualitatively different than salt‐free solutions. In the presence a two‐fold molar Na + (per phosphate group) spectrum poly(iG) closely resembles that gel Guo‐5′‐P and is characteristic an ordered helical structure in which guanine residues hydrogen bonded to one another. absence added salt poly(rG) characterized by unusual ring frequencies suggests tautomeric or ionic...
Abstract High levels of the vitamin K-dependent matrix Gla protein (MGP) have been found in calcified costal cartilage cow and vertebral soupfin shark (Galeorhinus galeus). In both species, MGP accounts for 35–40% total acid demineralization extract cartilage, mineral content is comparable to that bovine cortical bone. Shark are nearly insoluble neutral buffers, a conserved property indicates self-aggregation could be important as yet unknown function MGP. The complete amino sequence was...
2D NMR has been used to examine the structure and dynamics of a 12-mer DNA duplex, d(T1A2G3T4C5A6A7*G8G9G10C11A12)−d(T13G14C15C16C17T18T19G20A21C22T23A24), containing 10R adduct at dA*7that corresponds trans addition N6-amino group dA7 (−)-(7S,8R,9R,10S)-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene [(−)-(S,R,R,S)-BP DE-2]. This duplex contains base sequence for major dA mutational hot spot in HPRT gene when Chinese hamster V79 cells are given low doses highly carcinogenic...
Polycyclic aromatic hydrocarbons (PAHs) are widespread environmental contaminants whose metabolism in mammals results deleterious cell transformation. Covalent modification of DNA by diol epoxides metabolically formed from PAHs such as benzo[a]pyrene (BaP) provides a mechanism for the genotoxicity, mutagenicity, and carcinogenicity PAHs. We had previously reported NMR evidence minor conformer duplex d(G1G2T3C4A5*C6G7A8G9)·d(C10T11C12G13G14G15A16C17C18) containing dG14 mismatch opposite dA5*...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructural Basis for the Sequence-Specific DNA Strand Cleavage by Enediyne Neocarzinostatin Chromophore. Structure of Postactivated Chromophore-DNA ComplexXiaolian Gao, Adonis Stassinopoulos, Jeffrey S. Rice, and Irving H. GoldbergCite this: Biochemistry 1995, 34, 1, 40–49Publication Date (Print):January 10, 1995Publication History Published online1 May 2002Published inissue 10 January...