A5037083301- Renal Transplantation Outcomes and Treatments
- Systemic Lupus Erythematosus Research
- T-cell and B-cell Immunology
- Chemokine receptors and signaling
- Neurological Complications and Syndromes
- Pregnancy and Medication Impact
- Medication Adherence and Compliance
- Organ Donation and Transplantation
- Organ Transplantation Techniques and Outcomes
- Monoclonal and Polyclonal Antibodies Research
- Vasculitis and related conditions
- Pharmacological Effects and Toxicity Studies
- Renal Diseases and Glomerulopathies
- Electrolyte and hormonal disorders
- Immunodeficiency and Autoimmune Disorders
- Cytomegalovirus and herpesvirus research
- Liver Diseases and Immunity
- Immune Cell Function and Interaction
- Bipolar Disorder and Treatment
- NF-κB Signaling Pathways
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Adolescent and Pediatric Healthcare
- Family Caregiving in Mental Illness
- Glycogen Storage Diseases and Myoclonus
- Otitis Media and Relapsing Polychondritis
Medizinische Hochschule Hannover
2010-2022
Zentrum für Kinderheilkunde
2019
Feinstein Institute for Medical Research
2008
Columbia University
2006
Albert Einstein College of Medicine
2002-2004
Costimulatory blockade with CTLA4Ig and anti-CD40L along a single dose of cyclophosphamide induces remission systemic lupus erythematosus nephritis in NZB/W F(1) mice. To understand the mechanisms for impending relapse, we examined expression profiles 61 inflammatory molecules perfused kidneys treated mice untreated at different stages disease. Further studies using flow cytometry immunohistochemistry allowed us to determine cellular origins several key markers. We show that only limited set...
B cells have multiple roles in immune activation and inflammation separate from their capacity to produce antibodies. cell depletion is currently under intense investigation as a therapeutic strategy for autoimmune diseases. The TNF family members cell–activating factor of the (BAFF) its homolog A proliferation-inducing ligand (APRIL) are survival differentiation factors therefore rational targets. We compared effects BAFF receptor–Ig, which blocks only BAFF, with those transmembrane...
Abstract TNF-like weak inducer of apoptosis, or TWEAK, is a relatively new member the TNF-ligand superfamily. Ligation TWEAK receptor Fn14 by has proinflammatory effects on fibroblasts, synoviocytes, and endothelial cells. Several TWEAK-inducible cytokines are important in pathogenesis kidney diseases; however, whether can induce effect cells not known. We found that murine mesangial express cell surface receptor. stimulation led to dose-dependent increase CCL2/MCP-1, CCL5/RANTES,...
Abstract NZB/W F1 mice with established nephritis were treated a single dose of cyclophosphamide or without 2-wk course murine CTLA4Ig, either alone in combination anti-CD154. Sixty to 80% entered remission, and remission could be reinduced following relapse. A decrease the frequency anti-DNA-producing B cells activated T was observed mice, but this effect lasted only 3–6 wk, while remissions sustained for up 20 wk. Light microscopy kidneys revealed less glomerular inflammation, tubular...
Abstract B cell-activating factor belonging to the TNF family (BAFF) blockade prevents onset of disease in systemic lupus erythematosus (SLE)-prone NZB/NZW F1 mice. To determine mechanism this effect, we administered a short course TACI-Ig with and without six doses CTLA4-Ig 18- 20-wk-old mice evaluated effect on T cell subsets anti-dsDNA Ab-producing cells. Even brief exposure had beneficial murine SLE; potentiated effect. The combination resulted temporary decrease serum IgG levels....
Abstract Objective To determine the immunologic effects of anti‐CD154 (CD40L) therapy in (NZB × NZW)F 1 mouse model systemic lupus erythematosus. Methods Twenty‐week‐old and 26‐week‐old mice were treated with continuous therapy. Mice followed up clinically, their spleens studied at intervals for B T cell numbers subsets frequency anti–double‐stranded DNA (anti‐dsDNA)–producing cells. cell–dependent immunity was assessed by studying humoral response to hapten oxazolone. Results IgG anti‐dsDNA...
ABSTRACT. Smad6 and Smad7 are inhibitory SMADs with putative functional roles at the intersection of major intracellular signaling networks, including TGF-β, receptor tyrosine kinase (RTK), JAK/STAT, NF-κB pathways. This study reports differential regulation in TGF-β renal cells, murine models disease human glomerular diseases. is upregulated podocytes all examined diseases (focal segmental glomerulosclerosis [FSGS], minimal-change [MCD], membranous nephropathy [MNP], lupus nephritis [LN],...
Objectives. The chemokine CXCL13, also known as BCA-1 (B-cell-attracting chemokine-1) or BLC (B-lymphocyte chemoattractant), is a major regulator of B-cell trafficking. We have recently shown that excessive expression dendritic cell-derived CXCL13 distinctive early event for nephritis in murine model systemic lupus erythematosus (SLE). Furthermore, kidney biopsies from SLE patients, protein and mRNA are strongly expressed B-cell-containing inflammatory lesions. Here, we ask whether serum...
Abstract Background The majority of sera from patients with primary membranous nephropathy have autoantibodies against the M ‐type phospholipase A 2 receptor ( PLA R ) which is expressed on human podocytes. rabbit variant attaches to collagen type IV via fibronectin II domain, also present in . Design To assess whether 2R involved attachment , we conducted a cell adhesion assay collagen‐coated surface using ‐transfected and mock‐transfected embryonic kidney HEK cells. test hypothesis that...
Follow-up care after kidney transplantation is performed in transplant centers as well local nephrologist's practices Germany. However, organized integrated of these different sectors the German health system missing. This organizational deficit non-adherence recipients and longterm cardiovascular complications are major reasons for an impaired patient graft survival.The KTx360° study supported by a grant from Federal Joint Committee Republic The will include 448 (39 children) incident...
Podocytes maintain the structure and function of glomerular filtration barrier. However, podocytes have recently been implicated in innate immune response, their as non-haematopoietic antigen-presenting cells was highlighted. We shown previously that excessive expression chemokine CXCL13 is a distinctive early event for nephritis murine model systemic lupus erythematosus (SLE). Furthermore, we found elevated significantly serum patients with SLE-nephritis. In this study, were able to show...
Even though current treatment guidelines for idiopathic membranous glomerulonephritis (iMGN) exist, many questions regarding an optimal therapy remain unanswered. Complete remission cannot be achieved in all patients; relapses occur, some cases frequently, and side effects from the immunosuppressive are common. Therapeutic options high-risk patients not responding to standard therapies limited. Recent research reveals that human M-type phospholipase A2 receptor (PLA2 R) is a causative factor...
Background: Chemokine CXC ligand 13 (CXCL13) has been implicated in perivascular inflammation and pulmonary vascular remodeling patients with idiopathic artery hypertension (IPAH).We wondered whether CXCL13 may also play a role chronic thromboembolic (CTEPH) serum levels of might serve as biomarkers these conditions.Methods: Lung tissue from IPAH or CTEPH was immunostained for CXCL13.Serum samples were obtained (n = 42) 50) healthy controls 13).Serum concentrations measured by enzyme-linked...
AbstractNZW "×" BXSB F1 mice develop SLE that is associated with an anti-phospholipid syndrome characterized by anti-cardiolipin antibodies, thrombocytopenia and small coronary artery thrombosis. This immune mediated and, dependent, on CD4+T cells. To determine whether disease in these can be treated blockade of T cell costimulation we them the CD28 antagonist CTLA4Ig at 9 or 12 weeks age. completely prevented both nephritis myocardial infarcts if it was given age, before antibodies could...
The presence of B-cell clusters in allogenic T cell-mediated rejection (TCMR) kidney allografts is linked to more severe disease entities. In this study we characterized infiltrates patients with TCMR and examined the role serum CXCL-13 these experimentally. levels were analyzed 73 allograft recipients at time biopsy. addition, four evaluated for CXCL13 during first week after transplantation. ELISA was done measure levels. For further mechanistic understanding, a translational transplant...
Ischemia reperfusion injury (IRI) is linked with inflammation in kidney transplantation (ktx). The chemokine CXCL13, also known as B lymphocyte chemoattractant, mediates recruitment of cells within follicles lymphoid tissues and has recently been identified a biomarker for acute allograft rejection. goal this study was to explore whether IRI contributes the up-regulation CXCL13 levels ktx. It demonstrated that systemic were increased mouse models uni- bilateral renal IRI, which correlated...
Objective: There is solid evidence that kidney transplant (KTx) patients are susceptible to weight gain after transplantation. Post-transplantation obesity (BMI ≥ 30 kg/m2) seems be associated with higher risks of hypertension, dyslipidemia, diabetes mellitus, and cardiovascular events, while there contradicting findings regarding the association between mortality, graft failure transplantation as well other variables. We aimed evaluate course KTx assess prevalence post-transplant in a large...
Recent studies have demonstrated that CXCL13 serum levels correlate significantly with systemic lupus erythematosus (SLE) disease activity. However, experimental show production can also be induced by bacterial exposure as well in response to inflammatory cytokines. This report asks whether are elevated patients evidence of infections and there is a correlation the C-reactive protein (CRP) or severity illness critically ill patients. were compared 39 active SLE (without concomitant...
Abstract Background and objectives Internet-based technologies play an increasingly important role in the management outcome of patients with chronic kidney disease (CKD). The healthcare system is currently flooded digital innovations internet-based as a consequence coronavirus 2019 (COVID-19) pandemic. However, information about attitude German CKD-patients access to online tools towards use remote, interactions such video conferencing, email, electronic medical records apps general for...
Worldwide clinical guidelines for the care of kidney transplant (KT) recipients recognize importance health providers imparting appropriate immunosuppressive medication (ISM) information facilitation safe self-management. The extent made available is, however, not necessarily what patients require to know about their prescribed medicines. A useful indicator determining quality prescription practice is degree provided meets personal needs patients. No previous studies have focused on ISM KT...