A5060373504- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Virus-based gene therapy research
- Xenotransplantation and immune response
- Pluripotent Stem Cells Research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Liver physiology and pathology
- Mycobacterium research and diagnosis
- Organ Transplantation Techniques and Outcomes
- Judicial and Constitutional Studies
- Viral Infections and Immunology Research
- Innovations in Medical Education
- Radiology practices and education
- Amino Acid Enzymes and Metabolism
- Genetic and phenotypic traits in livestock
- Genetically Modified Organisms Research
- Diversity and Career in Medicine
- Epigenetics and DNA Methylation
- Immunodeficiency and Autoimmune Disorders
- Genetic Syndromes and Imprinting
Genus Oncology (United States)
2024
Genus (United States)
2018-2024
Genus (United Kingdom)
2024
University of Missouri
2011-2021
GenUs BioSystems (United States)
2018
United States Court of Appeals for the Ninth Circuit
2018
Harvard University Press
2018
Sidley Austin
2018
Library of Michigan
2018
University of Michigan
2018
Targeted modification of the pig genome can be challenging. Recent applications CRISPR/Cas9 system hold promise for improving efficacy editing. When a designed targeting CD163 or CD1D was introduced into somatic cells, it highly efficient in inducing mutations. these mutated cells were used with cell nuclear transfer, offspring modifications created. delivered vitro produced presumptive porcine zygotes, effective creating mutations eGFP, CD163, and (100% efficiency blastocyst stage embryos);...
Disease resistance genes in livestock provide health benefits to animals and opportunities for farmers meet the growing demand affordable, high-quality protein. Previously, researchers used gene editing modify porcine CD163 demonstrated a harmful virus that causes reproductive respiratory syndrome (PRRS). To maximize potential benefits, this disease trait needs be present commercially relevant breeding populations multiplication distribution of pigs. Toward goal, first-of-its-kind, scaled...
Porcine reproductive and respiratory syndrome (PRRS) is one of the world's most persistent viral pig diseases, with a significant economic impact on industry. PRRS affects pigs all ages, causing late-term abortions stillbirths in sows, disease piglets, increased susceptibility to secondary bacterial infection high mortality rate. caused by positive single-stranded RNA virus (PRRSV), which has narrow host-cell tropism limited monocyte-macrophage lineage cells. Several studies demonstrated...
The coronaviruses, porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis (TGEV), and deltacoronavirus (PDCoV) represent important sources of neonatal on pig farms. requirement for aminopeptidase N (APN) as a receptor TGEV, but not PEDV, is well established. In this study, the biological relevance APN PDCoV was tested by using CRISPR/Cas9 to knockout gene, ANPEP, in pigs. Porcine alveolar macrophages (PAMs) from ANPEP (KO) pigs showed resistance infection. However, lung...
Gene editing technologies have revolutionized the field of livestock breeding, offering unprecedented opportunities to enhance animal welfare, productivity, and sustainability. This paper provides a comprehensive review recent innovations applications gene in livestock, exploring diverse as well regulatory ethical considerations, current challenges prospects technology industry. Overall, this underscores transformative potential breeding its pivotal role shaping future agriculture biomedicine.
Recent advancements in gene editing techniques have increased number and utility. These are an attractive alternative to conventional targeting methods via homologous recombination due the ease of use high efficiency editing. We previously produced cytidine monophosphate-N-acetylneuraminic acid hydroxylase (CMAH) knockout (KO) pigs a Minnesota miniature pig genetic background. were generated using zinc-finger nucleases (ZFNs) combination with donor DNA containing total homology length 1600...
Although several techniques have been developed to create gene knockouts in pigs, homologous recombination will continue be required for site-specific genome modifications that are more sophisticated than disruption (base changes, domain exchanges, conditional knockouts). The objective of the present paper was improve efficiency porcine fetal fibroblasts, which would used produce knockout pigs by somatic cell nuclear transfer. A promoter-trap enable selection GGTA1 targeted cells. Cells were...
Abstract Mutations and single base pair polymorphisms in various genes have been associated with increased susceptibility to inflammatory bowel disease (IBD). We created a series of rat strains carrying targeted genetic alterations within three IBD genes: Nod2 , Atg16l1 Il23r, using CRISPR/Cas9 genome editing technology. Knock-out alleles known human were generated on different backgrounds: Fischer, Lewis Sprague Dawley. The availability these models will contribute our understanding the...
Production of Cas9 mRNA in vitro typically requires the addition a 5´ cap and 3´ polyadenylation. A plasmid was constructed that harbored T7 promoter followed by EMCV IRES coding region. We hypothesized use metastasis associated lung adenocarcinoma transcript 1 (Malat1) triplex structure downstream an IRES/Cas9 expression cassette would make polyadenylation produced unnecessary. sequence from mMalat1 gene cloned described above. An concentration curve with either commercially available or...
Abstract Livestock with enhanced disease resistance genes offer both health advantages to animals and economic opportunities for farmers aiming meet the rising demand affordable, high-quality protein. This study builds upon prior research that utilized gene editing modify porcine CD163 gene, demonstrating RNA virus responsible reproductive respiratory syndrome (PRRS). To amplify impact of this trait, it is crucial be present in commercially significant breeding populations, facilitating...
Gene editing techniques are increasing in their availability and ease of use. These an attractive alternative to conventional gene targeting methods via homologous recombination due the use efficiency targeting. We have previously produced CMP-N-acetylneuraminic acid hydroxylase (CMAH) knockout (KO) pigs a Minnesota miniature pig genetic background. were generated using zinc-finger nucleases (ZFN) combination with donor DNA containing total length homology 1600 bp (800-bp on each arm). A...
Every year more than 100 000 people need organ transplants and in 2009 there were only 20 139 deceased donors. Genetically modified swine may be able to fill this unmet clinical need. It is clear that multiple transgenes will required make porcine organs or cells compatible with human recipients. Efficiencies production of xenotransplantation pigs could gained if sequentially stacked at a single locus. The larger purpose project evaluate site-specific recombination system as tool add the α...
Gene targeting in mammalian cells has become a routine technique and is currently used to study gene function, create biomedical models, generate potential tissue sources for xenotransplantation. Severe combined immunodeficiency (SCID) condition characterised by the absence of T lack B cell function. affects ~1 out every 100 000 infants. Autosomal recessive SCID can occur due mutation within recombination activating genes (RAG-1/RAG-2) that play role immunoglobulins T-cell receptors. been...
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife