A5048905643- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Ovarian function and disorders
- Renal and related cancers
- Prenatal Screening and Diagnostics
- Reproductive Physiology in Livestock
- Birth, Development, and Health
- Reproductive Health and Technologies
- Assisted Reproductive Technology and Twin Pregnancy
- Genetic and phenotypic traits in livestock
- CRISPR and Genetic Engineering
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- 3D Printing in Biomedical Research
- Reproductive System and Pregnancy
- Tissue Engineering and Regenerative Medicine
- COVID-19 Impact on Reproduction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Pregnancy and preeclampsia studies
- Adipose Tissue and Metabolism
- Virus-based gene therapy research
- Xenotransplantation and immune response
- Mitochondrial Function and Pathology
Genus (United States)
2020-2025
Colorado Center for Reproductive Medicine
2014-2024
Genus (United Kingdom)
2021-2024
Genus Oncology (United States)
2024
Division of Undergraduate Education
2021
National Foundation for Fertility Research
2011-2019
University of Illinois Urbana-Champaign
2008-2015
University of Missouri
2012
University of Illinois System
2010
Urbana University
2009
Development of in vitro-produced bovine embryos was studied 3 two-step culture media: synthetic oviduct fluid (SOF), Gardner's G1/G2, and control (hamster embryo medium with 11 amino acids [HECM-6] followed by tissue 199 + 10% calf serum). Modifications were made to reduce or eliminate protein. Glycolysis Krebs cycle activity morulae blastocysts developed from selected immature oocytes measured. There no differences development the morula blastocyst stages between SOF, (41%, 36%, 46%,...
The objective of these experiments was to evaluate the importance fatty acid beta-oxidation (FAO) in cumulus oocyte complex (COC) during vitro maturation (IVM) nuclear and gene expression both cells three species with differing amounts intracellular lipids (mouse, low; bovine, moderate; porcine, high). We inhibited FAO using etomoxir at 0, 10, 25, 100, or 250 μM. Completion after COC exposure μM mouse oocytes, 100 bovine as little 10 porcine oocytes (P < 0.05). When COCs resulting inhibition...
The objective of this work was to determine the role mitochondria in loss oocyte quality with maternal aging. Our results show that mitochondrial DNA (mtDNA) copy number and function are reduced eggs from aged mice after both vivo vitro maturation. Higher incidences spindle abnormalities were observed old eggs. However, no correlation egg ATP content found. In matured did not have a normal cortical distribution active subject increased oxidative stress due higher levels reactive oxygen...
Single-cell RNA sequencing of cells from cultured human blastocysts has enabled us to define the transcriptomic landscape placental trophoblast (TB) that surrounds epiblast and associated embryonic tissues during enigmatic day 8 (D8) D12 peri-implantation period before villous placenta forms. We analyzed transcriptomes 3 early cell types, cytoTB (CTB), syncytioTB (STB), migratoryTB (MTB), picked manually embryos dissociated with trypsin were able follow sublineages emerged proliferating CTB...
Fatty acid β-oxidation (FAO) is essential for oocyte maturation in mice. The objective of this study was to determine the effect etomoxir (a FAO inhibitor; 100 μM), carnitine (1 mM), and palmitic or μM) during on metabolism gene expression cumulus cells, subsequent embryo development mouse. Carnitine significantly increased development, while there a decrease following with μM (P<0.05) treatment. Glucose consumption per cumulus-oocyte complex (COC) decreased after treatment (P<0.05)....
Abstract Reduced quality in oocytes from women of advanced maternal age (AMA) is associated with dysfunctional mitochondria. The objective this study was to investigate the mechanisms controlling mitochondrial during aging mouse and human oocytes. We first evaluated expression proteins involved unfolded protein response (UPRmt) mitophagy vivo matured metaphase II (MII) collected young aged mice. Expression UPRmt proteins, HSPD1 LONP1, total-PRKN phosphorylated-PRKN, significantly decreased...
Profiling bovine blastocyst transcriptome at the single-cell level has enabled us to reveal first cell lineage segregation, during which inner mass (ICM), trophectoderm (TE), and an undefined population of transitional cells were identified. By comparing blastocysts derived in vivo (IVV), vitro from a conventional culture medium (IVC), optimized reduced nutrient (IVR), we found delay fate commitment ICM IVC IVR embryos. Developmental potential differences between IVV, IVC, embryos mainly...
The effect of maturation in vitro on metabolism individual bovine oocytes was examined. Three media were used: standard, consisting tissue culture medium 199 supplemented with serum and pyruvate, a chemically defined either amino acids or lactate. Development to blastocyst significantly higher (P < 0.05) after standard (47%) than lactate (17%) but not different (29%). Glucose through the Krebs cycle (0.48, 0.43, 0.38 pmol/oocyte/3 hr, respectively) affected by removal unlabeled pyruvate from...
Glutathione (GSH) content in mature porcine oocytes is correlated with subsequent fertilization and developmental success. Adenosine triphosphate (ATP) an important energy source for maintaining cellular activities protein synthesis. The objective of this study was to compare GSH ATP concentrations vivo vitro matured oocytes. Ovulated, were frozen at -80 degrees C groups 10-20 or 5-10 (ATP). In either tissue culture medium-199 (TCM199) supplemented polyvinyl alcohol (PVA) hyaluronic acid...
The objectives of this study were to manipulate metabolism glucose through glycolysis and the pentose phosphate pathway (PPP) in porcine oocytes during vitro maturation, determine effects manipulation on meiotic progression, intracellular glutathione (GSX) concentrations embryonic development. Cumulus-oocyte complexes isolated from abattoir ovaries matured (40–44 h) Purdue Porcine Medium for maturation alone (control) or supplemented with pyrroline-5 carboxylate (PC, 0.1 μM; PPP stimulator),...
Genetic modification of germline stem cells (GSCs) is an alternative approach to generate large transgenic animals where GSCs are transplanted into a recipient testis donor-derived sperm. The objective the present study was explore application viral vectors in delivering enhanced green fluorescent protein (EGFP) transgene for production gametes through germ cell transplantation. Both adeno-associated virus (AAV)- and lentivirus (LV)-based were effective transducing pig GSCs, resulting sperm...
Maternal aging results in reduced oocyte and blastocyst quality, thought to be due, part, mitochondrial dysfunction accumulation of reactive oxygen species. To reduce oxidative stress, the antioxidants α-lipoic acid (ALA; 10 µM), α-tocopherol (250 hypotaurine (1 mM) N-acetylcysteine (NAC; 1 mM), sirtuin (100 ng mL–1) were added embryo culture medium (AntiOX) compared with a control (CON) without assess development after vitro maturation fertilisation oocytes from aged B6D2F1 female mice...
The advent of metabolomics technology and its application to small samples has allowed us non-invasively monitor the metabolic activity embryos in a complex culture environment. aim this study was apply analysis individual from several species during vitro development gain an insight into pathways used by their relationship with embryo quality. Alanine is produced both vivo- vitro-derived human, murine, bovine porcine embryos. Glutamine also these four species, but only those vitro. Across...