A5025684532- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- T-cell and Retrovirus Studies
- Animal Disease Management and Epidemiology
- Immunotherapy and Immune Responses
- DNA and Nucleic Acid Chemistry
- Asthma and respiratory diseases
- Vector-Borne Animal Diseases
- Air Quality and Health Impacts
- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- IL-33, ST2, and ILC Pathways
- Immune cells in cancer
- Pediatric health and respiratory diseases
- vaccines and immunoinformatics approaches
- Neuropeptides and Animal Physiology
- Carcinogens and Genotoxicity Assessment
- Nanoplatforms for cancer theranostics
- Inhalation and Respiratory Drug Delivery
- Telomeres, Telomerase, and Senescence
- Occupational and environmental lung diseases
- Genomics and Phylogenetic Studies
- Occupational exposure and asthma
- Neonatal Respiratory Health Research
Yamaguchi University
2023-2024
University of the Ryukyus
2021-2022
Kindai University
2020
Kawasaki Medical School
2016-2017
Hokkaido University
2011-2014
Human T cell leukemia virus type 1 (HTLV-1) basic leucine zipper factor (HBZ), which is encoded by a minus strand mRNA, thought to play important roles in the development of adult T-cell (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). However, comprehensive analysis HBZ, including mRNA protein expression, humoral immunoreactivity against HTLV-1 proviral load (PVL), HTLV-1-infected individuals with different clinical status has not been reported previously. In...
The adoptive transfer of OVA-specific Th1 cells into WT mice followed by OVA inhalation induces a significant elevation airway hyper-responsiveness (AHR) with neutrophilia but not mucus hypersecretion. Here, we demonstrate that the inflammation model, pathogenically characterized as severe asthma, was partly mimicked i.n. administration IFN-γ. IFN-γ instead caused AHR neutrophilia, and remarkably induced neurokinin-2 receptor (NK2R) expression along neurokinin A (NKA) production in lung. To...
Adoptive cell transfer of an ovalbumin (OVA)-specific Th17-polarized population from transgenic DO11.10 mice into BALB/c followed by OVA inhalation caused airway hyperresponsiveness (AHR) with severe neutrophilia. The transferred Th17 population—previously polarized in vitro IL-6, transforming growth factor-β and IL-23—contained negligible numbers IFN-γ-producing cells; however, during Th17-cell-dependent inflammation, significant cells—including cells producing both IL-17 IFN-γ only...
New lipid conjugates of DNA and RNA incorporating one to four [(4-dodecylphenyl)sulfonyl]phosphoramidate or (hexadecylsulfonyl)phosphoramidate groups at internucleotidic positions near the 3′ 5′-end were synthesized characterized. Low cytotoxicity their ability be taken up into cells without transfection agents demonstrated. Lipid-conjugated siRNAs targeting repulsive guidance molecules a (RGMa) have shown comparable gene silencing activity in PK-59 unmodified control siRNA when delivered...
In this study, the efficiency of RNA interference small interfering RNAs (siRNAs) bearing 5'-O-methyl-2'-deoxythymidine (X) and 5'-amino-2', 5'-dideoxythymidine (Z) at 5'-end sense strand antisense siRNA was investigated in HeLa cells stably expressing enhanced green fluorescent protein. The results indicated that when one modified with X or Z other unmodified, modification predominant process selection unmodified selected as a guide strand. When both strands are Z, will be certain...
Understanding of the interactions between macrophages and multifunctional nanoparticles is important for development novel macrophage-based immunotherapies. Here, we investigated effects fluorescent thiol-organosilica particle size surface properties on cell-particle interactions, including mitochondrial activity, using mouse macrophage cell line J774A.1. Three different sizes particles (150, 400, 680 nm in diameter) containing fluorescein (OS/F150, OS/F400, OS/F680) functionalized with...
The bacterium "Candidatus Hydrogenosomobacter endosymbioticus" is an intracellular symbiont of anaerobic scuticociliate GW7, which associated with hydrogenosome together methanogenic archaea. Here, we report a complete genome sequence the consisting 827 kbp. Knowing this would contribute to understanding metabolic interactions and evolution tripartite symbiosis.
We investigated the distribution of intratracheally administered thiol-organosilica (thiol-OS) particles in mouse lungs. Toward this end, single doses thiol-OS containing fluorescein (140 nm diameter) (F140) and rhodamine B (Rh) (Rh160, Rh280, Rh420, Rh640, Rh1630 with diameters 160, 280, 420, 640, 1630 nm, respectively) were administered. After 24 h, fluorescence imaging revealed homogeneous a patchier pattern on lung surface no difference among six particle sizes. Simultaneous dual...
Telomerase activity has been regarded as a critical step in cellular immortalization and carcinogenesis because of this, regulation telomerase represents an attractive target for anti-tumor specific therapeutics. Recently, one avenue cancer research focuses on antisense strategy to the oncogenes or driver genes, sequence fashion down-regulate expression gene. The protein catalytic subunit, human reverse transcriptase (hTERT) template RNA component (hTERC) are essential function, thus...
Detection of specific immune responses against cancer/testis antigen NY-ESO-1 was recently reported in patients with adult T-cell leukemia/lymphoma (ATL) and human leukemia virus type 1 (HTLV-1)-infected asymptomatic carriers (ACs). However, the relationship HTLV-1 proviral load (PVL) levels viral gene expression remain unclear.We measured plasma autoantibodies to immunogenic tumor HTLV-1-infected individuals different clinical status, healthy controls. Data were compared tax HBZ mRNA...