A5103067596- Biochemical and Molecular Research
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- HIV/AIDS drug development and treatment
- interferon and immune responses
- HIV Research and Treatment
Xiamen University
2024
Mutations in a Plasmodium de-ubiquitinase UBP1 have been linked to antimalarial drug resistance. However, the UBP1-mediated drug-resistant mechanism remains unknown. Through selection, genetic mapping, allelic exchange, and functional characterization, here we show that simultaneous mutations of two amino acids (I1560N P2874T) yoelii can mediate high-level resistance mefloquine, lumefantrine, piperaquine. Mechanistically, double are shown impair cytoplasmic aggregation de-ubiquitinating...
Eukaryotic genes contain introns that are removed by the spliceosomal machinery during mRNA maturation. Introns impose a huge energetic burden on cell; therefore, they must play an essential role in maintaining genome stability and/or regulating gene expression. Many (> 50%) Plasmodium parasites predicted introns, including 5' and 3' untranslated regions (UTR). However, roles of UTR expression malaria remain unknown. In this study, episomal dual-luciferase assay was developed to evaluate...