A5001083881- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
Northwestern University
2022-2023
It is known that microtubule-binding proteins including the Ska1 complex and DNA replication licensing factor, Cdt1, enable kinetochore-localized Ndc80 to form robust kinetochore-microtubule attachments. However, it not clear how stably coupled dynamic spindle microtubule plus-ends. Here, we have developed a conditional auxin-inducible degron approach reveal function for Cdt1 in chromosome segregation kinetochore–microtubule interactions separable from its role licensing. Further,...
Abstract The Ndc80 kinetochore complex is essential for robust kinetochore-microtubule (k-MT) attachments during mitosis. has been shown to recruit the Ska1 kinetochores, where thought aid in k-MT coupling by Ndc80. Our previous work that Cdt1, a DNA replication licensing factor, novel mitotic spindle-associated protein also recruited kinetochores via and required stabilizing attachments. In this study, we developed auxin-induced degron (AID)-tagging validate previously demonstrated role of...